plasmid mruby2 n1 Search Results


mruby2 n1 vector  (Addgene inc)
93
Addgene inc mruby2 n1 vector
Mruby2 N1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mruby2 n1 vector/product/Addgene inc
Average 93 stars, based on 1 article reviews
mruby2 n1 vector - by Bioz Stars, 2026-05
93/100 stars
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mruby2  (Addgene inc)
94
Addgene inc mruby2
(A) Top: neuron-specific viral delivery of a stable fluorophore <t>(mRuby2)</t> and a Ca 2+ sensor (GCaMP6s) along with the experimental design describing the acquisitions of z-stacks and singleplane t-series. Bottom: representative dual-color images of mRuby2 and GCaMP6s expressing cortical neurons before (0’), during (10’), and after (40’) NMDA application, with ANMAF-generated ROIs overlayed on the baseline image. (B) Synchronized Ca 2+ activity (ΔF/F 0 ) heatmap of neurons during NMDA application (n=27 neurons from 1 slice, Video 1) . (C) Long-lasting increase in GCaMP6s signals along with (D) persistent elevation of the neuronal area over 40’ after NMDA treatment, detected using both GCaMP6s and mRuby2 (n [mice: slices: neurons] =2:3:54). (E) Representative images of GCaMP6s-expressing cortical neurons at different time points (0’, 10’, and 40’) using transgenic Thy1-GCaMP6s mice. Insets : A single neuron at higher magnification shows persistent elevation in Ca 2+ . (F) Matched comparisons of neuronal areas between multiple time points, showing persistent swelling in most neurons. Statistical analysis: one-way ANOVA with Dunnett’s post-test, F (2.8, 261) =49.6, p<0.0001. (G) Significant and persistent increase in the neuronal areas after NMDA application, prevented by AP5 treatment. Statistical analysis: two-way ANOVA with Tukey’s post-test, F ( , 1020) =12.1, p<0.0001; n = 5:8:97 (aCSF), n = 6:11:97 (NMDA), n = 3:5:73 (AP5). (H) Increase in areas of paired neurons at 40’ after NMDA application (Δ Area, 40’ minus 0’), rescued partially by AP5. Statistical analysis: Kruskal-Wallis test with Dunn’s post-test, p<0.0001, ‘n’ same as Figure1f . (I) Distribution of total neuronal Δ Area at 40’, (n: aCSF=579 neurons, NMDA=315, AP5=123). Data represented as mean ± 95% CI (parametric data) or median ± IQR (non-parametric data). Scale bar: 50 µm. See also Supplemental Figure 1 and .
Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mruby2/product/Addgene inc
Average 94 stars, based on 1 article reviews
mruby2 - by Bioz Stars, 2026-05
94/100 stars
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mruby2-n1 3xflag (plasmid #118980)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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mruby2-nt-n1 (plasmid #54612)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


(A) Top: neuron-specific viral delivery of a stable fluorophore (mRuby2) and a Ca 2+ sensor (GCaMP6s) along with the experimental design describing the acquisitions of z-stacks and singleplane t-series. Bottom: representative dual-color images of mRuby2 and GCaMP6s expressing cortical neurons before (0’), during (10’), and after (40’) NMDA application, with ANMAF-generated ROIs overlayed on the baseline image. (B) Synchronized Ca 2+ activity (ΔF/F 0 ) heatmap of neurons during NMDA application (n=27 neurons from 1 slice, Video 1) . (C) Long-lasting increase in GCaMP6s signals along with (D) persistent elevation of the neuronal area over 40’ after NMDA treatment, detected using both GCaMP6s and mRuby2 (n [mice: slices: neurons] =2:3:54). (E) Representative images of GCaMP6s-expressing cortical neurons at different time points (0’, 10’, and 40’) using transgenic Thy1-GCaMP6s mice. Insets : A single neuron at higher magnification shows persistent elevation in Ca 2+ . (F) Matched comparisons of neuronal areas between multiple time points, showing persistent swelling in most neurons. Statistical analysis: one-way ANOVA with Dunnett’s post-test, F (2.8, 261) =49.6, p<0.0001. (G) Significant and persistent increase in the neuronal areas after NMDA application, prevented by AP5 treatment. Statistical analysis: two-way ANOVA with Tukey’s post-test, F ( , 1020) =12.1, p<0.0001; n = 5:8:97 (aCSF), n = 6:11:97 (NMDA), n = 3:5:73 (AP5). (H) Increase in areas of paired neurons at 40’ after NMDA application (Δ Area, 40’ minus 0’), rescued partially by AP5. Statistical analysis: Kruskal-Wallis test with Dunn’s post-test, p<0.0001, ‘n’ same as Figure1f . (I) Distribution of total neuronal Δ Area at 40’, (n: aCSF=579 neurons, NMDA=315, AP5=123). Data represented as mean ± 95% CI (parametric data) or median ± IQR (non-parametric data). Scale bar: 50 µm. See also Supplemental Figure 1 and .

Journal: bioRxiv

Article Title: Brief excitotoxic insults cause a calpain-mediated increase in nuclear membrane permeability in neonatal neurons

doi: 10.1101/2023.08.22.554167

Figure Lengend Snippet: (A) Top: neuron-specific viral delivery of a stable fluorophore (mRuby2) and a Ca 2+ sensor (GCaMP6s) along with the experimental design describing the acquisitions of z-stacks and singleplane t-series. Bottom: representative dual-color images of mRuby2 and GCaMP6s expressing cortical neurons before (0’), during (10’), and after (40’) NMDA application, with ANMAF-generated ROIs overlayed on the baseline image. (B) Synchronized Ca 2+ activity (ΔF/F 0 ) heatmap of neurons during NMDA application (n=27 neurons from 1 slice, Video 1) . (C) Long-lasting increase in GCaMP6s signals along with (D) persistent elevation of the neuronal area over 40’ after NMDA treatment, detected using both GCaMP6s and mRuby2 (n [mice: slices: neurons] =2:3:54). (E) Representative images of GCaMP6s-expressing cortical neurons at different time points (0’, 10’, and 40’) using transgenic Thy1-GCaMP6s mice. Insets : A single neuron at higher magnification shows persistent elevation in Ca 2+ . (F) Matched comparisons of neuronal areas between multiple time points, showing persistent swelling in most neurons. Statistical analysis: one-way ANOVA with Dunnett’s post-test, F (2.8, 261) =49.6, p<0.0001. (G) Significant and persistent increase in the neuronal areas after NMDA application, prevented by AP5 treatment. Statistical analysis: two-way ANOVA with Tukey’s post-test, F ( , 1020) =12.1, p<0.0001; n = 5:8:97 (aCSF), n = 6:11:97 (NMDA), n = 3:5:73 (AP5). (H) Increase in areas of paired neurons at 40’ after NMDA application (Δ Area, 40’ minus 0’), rescued partially by AP5. Statistical analysis: Kruskal-Wallis test with Dunn’s post-test, p<0.0001, ‘n’ same as Figure1f . (I) Distribution of total neuronal Δ Area at 40’, (n: aCSF=579 neurons, NMDA=315, AP5=123). Data represented as mean ± 95% CI (parametric data) or median ± IQR (non-parametric data). Scale bar: 50 µm. See also Supplemental Figure 1 and .

Article Snippet: Co-expression of GCaMP6s and mRuby2 in neocortical and hippocampal neurons was achieved by injecting pAAV-hSyn1-mRuby2GSG-P2A-GCaMP6s one week before imaging ( Figure1A ; Addgene, catalog # 50942-AAV1; 1.2×10 13 GC/mL).

Techniques: Expressing, Generated, Activity Assay, Transgenic Assay