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Image Search Results
Journal: Cell reports
Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis
doi: 10.1016/j.celrep.2024.114789
Figure Lengend Snippet: (A and G) Time course of prolactin (A) or SOD3 (G) osmotic pump embedding and maternal behavior tests in placental Sod3 KO dam. (B–F and H–L) Plasma levels of prolactin (B and H), number of living pups (C and I), latency of the first retrieval on day 1 (D and J), time spent grooming on day 1 (E and K), and time spent crouching on day 1 (F and L) of WT dams and KO dams with prolactin (B–F) or SOD3 (H–L) osmotic pump embedding of saline or recombinant prolactin. (M–P) mRNA expression levels of prolactin ( Prl ) (M and N) and prolactin receptor ( Prlr ) (O and P) in 200 ng/mL recombinant SOD3-stimulated GH3 cells (M and O) and mouse primary pituitary cells (N and P). N = 5 in each group; three technical replicates for each group; * p < 0.05 and ** p < 0.01.
Article Snippet: Osmotic pumps were filled with 7.5 μg of
Techniques: Clinical Proteomics, Saline, Recombinant, Expressing
Journal: Cell reports
Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis
doi: 10.1016/j.celrep.2024.114789
Figure Lengend Snippet: (A) Reactome pathway analysis of placental Sod3 f/f and Sod3 −/− dams. (B) Prl mRNA expression levels of 100 ng/mL recombinant fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 μM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; ** p < 0.01 vs. FGF1-control, †† p < 0.01 vs. FGF2-control, and ‡‡ p < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3 f/f and Sod3 −/− dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3 f/f and Sod3 −/− dams. N = 3 in each group; three biological and technical replicates for each group.
Article Snippet: Osmotic pumps were filled with 7.5 μg of
Techniques: Expressing, Recombinant, Control, Phospho-proteomics
Journal: Cell reports
Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis
doi: 10.1016/j.celrep.2024.114789
Figure Lengend Snippet:
Article Snippet: Osmotic pumps were filled with 7.5 μg of
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Catecholamine ELISA, Clinical Proteomics, Sandwich ELISA, Dehydrogenase Assay, Activity Assay, Software