Journal: Scientific Reports

Article Title: Inflammatory Eicosanoids Increase Amyloid Precursor Protein Expression via Activation of Multiple Neuronal Receptors

doi: 10.1038/srep18286

Figure Lengend Snippet: hTP-hAPP cells were transfected with 50 nM of control siRNA or siRNA directed to Gα q , Gα 12 , or Gα 13 and cultured for 72 h, with IBOP (10 nM) added over the last 48 h. ( A ) qRT-PCR analysis revealed a reduction in IBOP-induced APP mRNA levels only in cells treated with Gα q siRNA [R.Q. (relative quantification) values of APP/GAPDH from qPCR are plotted relative to non-IBOP-treated cells without siRNA addition]. ( B ) Only cells treated with Gα q siRNA showed reduced APP protein expression, as determined by immunoblot analysis with 5685 antibody [values are relative to non-IBOP-treated cells without siRNA addition, with normalization to α-tubulin]. Statistical analyses consisted of a mixed-effects model, with values representing estimates from the least squares means fit of the mixed procedure from 2–6 independent studies with 1–3 replicates for each treatment/study. Error bars represent SEM; **p < 0.01; ***p < 0.001.

Article Snippet: The following siRNAs were used: Cntl, Gαq and PKCβ siRNA (Santa Cruz Biotechnologies, Inc.; Dallas, TX); Gα and Gα 13 (Thermo Scientific; Waltham, MA); and PKCε and PKCα (Qiagen; Hilden, Germany).

Techniques: Transfection, Control, Cell Culture, Quantitative RT-PCR, Quantitative Proteomics, Expressing, Western Blot