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Thermo Fisher
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Bioss
pitx3 primary antibody Pitx3 Primary Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/pitx3/10__1007_slash_s11726___022___1343___3-57-127-136?v=Bioss Average 94 stars, based on 1 article reviews
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Addgene inc
pgk promoter puror pa expression cassette Pgk Promoter Puror Pa Expression Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/pitx3/pm28082457-173-2-10?v=Addgene+inc Average 91 stars, based on 1 article reviews
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Cusabio
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Addgene inc
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Thermo Fisher
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Thermo Fisher
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Cusabio
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Thermo Fisher
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The Company of Biologists
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Prolonged Differentiation of Neuron-Astrocyte Co-Cultures Results in Emergence of Dopaminergic Neurons
doi: 10.3390/ijms24043608
Figure Lengend Snippet: Gene expression over the course of neuronal-glial differentiation. ( A ) Timeline of the culture protocol and time points of sampling. Arrow indicates replating to a new culture plate. ( B ) Gene expression over time of a selection of dopaminergic markers for cell types relative to the neural progenitor cell (NPC) culture, ordered by expected timing of expression: early markers for differentiation of dopaminergic progenitor cells ( LMX1B ), differentiation of dopaminergic neurons ( NURR1 , PITX3 ), a catecholaminergic neuron marker ( TH ) and proteins that can transport dopamine ( SLC618A2 , SLC6A3 ) or are involved in dopaminergic neurotransmission ( KCNJ6 ). ( C ) Gene expression over time of a selection of markers for cell types relative to the NPC culture: NPC ( NEUROG1 , NES ), neurons ( TUBB3 , MAP2 ), astrocytes ( GFAP ), synapses ( SYNPR , DLG4 ), excitatory ( SLC17A6 ) and inhibitory ( SLC32A1 ) neurotransmitter vesicles. NP: neural progenitor medium, ND: neural differentiation medium CNTF: Ciliary neurotrophic factor. Significance (adjusted p -value ≤ 0.05) is indicated relative to *: NPC, ^: 28 days, ~: 42 days, #: 56 days, +: 63 days. Full statistics can be found in .
Article Snippet: Paired Like homeodomain 3 , PITX3 , Dopaminergic differentiation TF ,
Techniques: Gene Expression, Sampling, Selection, Expressing, Marker
Journal: International Journal of Molecular Sciences
Article Title: Prolonged Differentiation of Neuron-Astrocyte Co-Cultures Results in Emergence of Dopaminergic Neurons
doi: 10.3390/ijms24043608
Figure Lengend Snippet: Primers used for gene expression experiments with corresponding marker function and assay ID. All primers were purchased at Applied Biosystems. TF: transcription factor.
Article Snippet: Paired Like homeodomain 3 , PITX3 , Dopaminergic differentiation TF ,
Techniques: Gene Expression, Marker
Journal: Frontiers in Endocrinology
Article Title: Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells
doi: 10.3389/fendo.2019.00623
Figure Lengend Snippet: Genes dominantly and specifically expressed in anterior pituitary cell types.
Article Snippet:
Techniques:
Journal: Frontiers in Endocrinology
Article Title: Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells
doi: 10.3389/fendo.2019.00623
Figure Lengend Snippet: Specific groups of genes expressed in anterior pituitary cells.
Article Snippet:
Techniques: Marker
Journal: Frontiers in Endocrinology
Article Title: Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells
doi: 10.3389/fendo.2019.00623
Figure Lengend Snippet: Validation of expression for selected genes by qRT-PCR. (A) Measurement of expression by qRT-PCR of 19 genes in dispersed cells is highly correlated with that measured in anterior pituitary tissue from diestrus female rats. (B) Correlation of measurements of mean expression in dispersed pituitary cells by scRNA-seq and qRT-PCR. Genes represented: Caly, Cga, Fshb, Gata2, Gfap, Gh1, Lhb, Lhx3, Lhx4, Pitx3, Pomc, Prl, S100b, Sez6l2, Snap25, Sox2, Stmn3, Tmem130 , and Tshb ; expression values are shown in . (C) Validation of 10 genes identified as sexually dimorphic in scRNA-seq by qRT-PCR expression measurement in dispersed cells (compare with ). Asterisks indicate statistical significance at the p = 0.05 level by two-sample t -test with unequal variances, n = 6 samples per sex.
Article Snippet:
Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR