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Image Search Results
Journal: Oncotarget
Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain
doi: 10.18632/oncotarget.26432
Figure Lengend Snippet: ( A ) PIP strip nitrocellulose membrane with spotted lipids (100 pmol per spot). ( B ) PIP strips were blocked and probed with the indicated purified p85α protein (37 nM). Bound proteins were detected with an anti-p85α BH antibody, followed with an infrared secondary antibody and visualized using a LICOR Odyssey infrared scanner. PI = phosphatidylinositol; PI3P = phosphatidylinositol 3-phosphate; PI4P = phosphatidylinositol 4-phosphate; PI5P = phosphatidylinositol 5-phosphate; PI3,4P 2 = phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 = phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 = phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 = phosphatidylinositol 3,4,5-trisphosphate.
Article Snippet: Nitrocellulose-immobilized phospholipids (100 pmol per spot, PIP-Strips;
Techniques: Stripping Membranes, Purification
Journal: Oncotarget
Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain
doi: 10.18632/oncotarget.26432
Figure Lengend Snippet: ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
Article Snippet: Nitrocellulose-immobilized phospholipids (100 pmol per spot, PIP-Strips;
Techniques: Fluorescence, Labeling, Mutagenesis
Journal: Oncotarget
Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain
doi: 10.18632/oncotarget.26432
Figure Lengend Snippet: ( A ) PIP strip nitrocellulose membrane with spotted lipids (100 pmol per spot). ( B ) PIP strips were blocked and probed with the indicated purified p85α protein (37 nM). Bound proteins were detected with an anti-p85α BH antibody, followed with an infrared secondary antibody and visualized using a LICOR Odyssey infrared scanner. PI = phosphatidylinositol; PI3P = phosphatidylinositol 3-phosphate; PI4P = phosphatidylinositol 4-phosphate; PI5P = phosphatidylinositol 5-phosphate; PI3,4P 2 = phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 = phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 = phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 = phosphatidylinositol 3,4,5-trisphosphate.
Article Snippet: Nitrocellulose-immobilized phospholipids (100 pmol per spot, PIP-Strips;
Techniques: Stripping Membranes, Purification
Journal: Oncotarget
Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain
doi: 10.18632/oncotarget.26432
Figure Lengend Snippet: ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
Article Snippet: Nitrocellulose-immobilized phospholipids (100 pmol per spot, PIP-Strips;
Techniques: Fluorescence, Labeling, Mutagenesis
Journal: Oncotarget
Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain
doi: 10.18632/oncotarget.26432
Figure Lengend Snippet: ( A ) PIP strip nitrocellulose membrane with spotted lipids (100 pmol per spot). ( B ) PIP strips were blocked and probed with the indicated purified p85α protein (37 nM). Bound proteins were detected with an anti-p85α BH antibody, followed with an infrared secondary antibody and visualized using a LICOR Odyssey infrared scanner. PI = phosphatidylinositol; PI3P = phosphatidylinositol 3-phosphate; PI4P = phosphatidylinositol 4-phosphate; PI5P = phosphatidylinositol 5-phosphate; PI3,4P 2 = phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 = phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 = phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 = phosphatidylinositol 3,4,5-trisphosphate.
Article Snippet: Nitrocellulose-immobilized phospholipids (100 pmol per spot, PIP-Strips;
Techniques: Stripping Membranes, Purification
Journal: Oncotarget
Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain
doi: 10.18632/oncotarget.26432
Figure Lengend Snippet: ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
Article Snippet: Nitrocellulose-immobilized phospholipids (100 pmol per spot, PIP-Strips;
Techniques: Fluorescence, Labeling, Mutagenesis