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Image Search Results
Journal: Bioscience Reports
Article Title: A novel mass assay to measure phosphatidylinositol-5-phosphate from cells and tissues
doi: 10.1042/bsr20192502
Figure Lengend Snippet: Figure 2. LC-MS/MS based assay reveals that GST-PIP4Kα is highly specific for PI5P and has lower affinity for PI3P
Article Snippet: diC16-PI3P - Echelon P-3016; diC16-PI5P - Echelon P-5016; Avanti 850173 | rac-16:0 PI(5)P-d5 (custom synthesized); 17: 0 20: 4
Techniques: Liquid Chromatography with Mass Spectroscopy
Journal: Cells
Article Title: The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus
doi: 10.3390/cells10040848
Figure Lengend Snippet: MPRIP specifically interacts with Phosphatidylinositol 4,5-bisphosphate (PIP2). Various PIP-coated agarose beads were used as a bait to pull-down interacting proteins from HeLa nuclear fraction. The membrane was WB analyzed with anti-MPRIP antibody. I—input 2%; C—control beads; 3—PI(3)P, 4—PI(4)P; 5—PI(5)P; 3,4—PI(3,4)P2; 3,5—PI(3,5)P2; 4,5—PI(4,5)P2; 3,4,5—PI(3,4,5)P3. n = 3.
Article Snippet: The PIP-coated beads were obtained from
Techniques: