Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of the rack-compatible swim tunnel with metered flow. Following a 5-minute acclimation period within the tunnel, 12 mpf zebrafish were subjected to increasing flow rate intervals for a total of 1 hour. (B) Cartoon depiction of an ECG recording and a representative ECG trace from an anesthetized fish placed ventral-side up in a sponge surrounded by anesthesia solution. (C) Representative ECG traces for wild type and irf8 mutant zebrafish following the swim assay. Black arrows indicate unique electrical disturbances occurring above any baseline noise. (D) Percentage of fish with an abnormal electrical event during the ECG recording. Male n = 7 per genotype, ****p < 0.0001. Female n = 4-8 per genotype, ****p < 0.0001. Fisher’s exact test. (E) Categorization of ECG abnormalities observed throughout all wild type and irf8 mutant fish.

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Mutagenesis

Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of an adult zebrafish undergoing an echocardiogram with the transducer in the long-axis (LAX) position. Echocardiograms were performed on unchallenged fish. (B) Cartoon of an adult zebrafish heart from the perspective of the echocardiography recording, denoting the atrium (A), atrioventricular (AV) valve, ventriculobulbar (VB) valve, and bulbus arteriosus (BA). The boxes around the AV valve and VB valve depict the gating strategies to determine inward flow and outward flow of blood from the ventricle. (C) Still image from an adult zebrafish echocardiography recording with the ventricle and bulbus arteriosus outlined in red. (D) Representative echocardiogram trace gating on the AV valve with the early filling (E) wave, late filling (A) wave, diastasis time, isovolumic contraction time (IVCT), aortic ejection time (AET), and isovolumic relaxation time (IVRT) labeled. (E-G) Quantifications of IVRT (E), AET (F), and pressure within the VB valve (G) in male and female wild type and irf8 mutant fish at 6 and 12 mpf. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Each point represents individual beats per fish. 8-10 beats were analyzed per fish. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (H) Quantification of heart in male and female wild type and irf8 mutants. (I) Representative echocardiogram traces from a wild type and irf8 mutant recording. (J-M) Quantification of diastasis time in wild type males (J) and females (K) at 6 mpf and 12 mpf, as well as diastasis time in irf8 mutant males (L) and females (M) at 6 mpf and 12 mpf. A Gaussian distribution was created by analyzing 8 beats per sample and grouping diastasis duration in defined increments. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Horizontal bars represent the range of diastasis duration. (N-O) 95% confidence interval of the average diastasis time durations in wild type (gray) and irf8 mutant (blue) males (N) and females (O).

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Labeling, Mutagenesis

Journal: Biology

Article Title: Regional Phenotypic Differences of the Opener Muscle in Procambarus clarkii : Sarcomere Length, Fiber Diameter, and Force Development

doi: 10.3390/biology9060118

Figure Lengend Snippet: Side view of the chelated first walking leg. pushing on the transducer pin resting on the top of the dactylopodite. This side view shows the location of the apodemes for opener and closer muscles in the propodite. The force transducer pin rests on the bump on the dactylopodite.

Article Snippet: The transducer was connected to an ADInstruments Bridge Amplifier (ML221), and the support rod of the transducer was attached to a micromanipulator to allow for precise positioning.

Techniques: Muscles

Journal: Biology

Article Title: Regional Phenotypic Differences of the Opener Muscle in Procambarus clarkii : Sarcomere Length, Fiber Diameter, and Force Development

doi: 10.3390/biology9060118

Figure Lengend Snippet: Recording chamber showing pinning of preparation and suction electrode: ( A ) The photograph shows a dorsally dissected preparation pinned in the recording dish with the force transducer pin resting on the dactylopodite. The suction electrode holding the excitatory nerve can be seen in the bottom center of the dish, and the inhibitory nerve is floating free. ( B ) A diagram detailing the recording chamber setup specifically showing how the preparation is pinned between the dactylopodite and ventral propodite.

Article Snippet: The transducer was connected to an ADInstruments Bridge Amplifier (ML221), and the support rod of the transducer was attached to a micromanipulator to allow for precise positioning.

Techniques:

Journal: Biology

Article Title: Regional Phenotypic Differences of the Opener Muscle in Procambarus clarkii : Sarcomere Length, Fiber Diameter, and Force Development

doi: 10.3390/biology9060118

Figure Lengend Snippet: Dorsal dissection of first walking leg with force transducer location: ( A ) Photograph of the first walking leg showing location of the force transducer hook resting on the dactylopodite. ( B ) Diagram detailing anatomy of the preparation showing the pinnate arrangement of the opener muscle and its attachment to the apodeme.

Article Snippet: The transducer was connected to an ADInstruments Bridge Amplifier (ML221), and the support rod of the transducer was attached to a micromanipulator to allow for precise positioning.

Techniques: Dissection

Journal: Biology

Article Title: Regional Phenotypic Differences of the Opener Muscle in Procambarus clarkii : Sarcomere Length, Fiber Diameter, and Force Development

doi: 10.3390/biology9060118

Figure Lengend Snippet: Force transducer “L”-shaped pin design: This pin rests on the bump on the dactylopodite and relays the force to the transducer as the cheliped opens with stimulation.

Article Snippet: The transducer was connected to an ADInstruments Bridge Amplifier (ML221), and the support rod of the transducer was attached to a micromanipulator to allow for precise positioning.

Techniques:

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Long-term incubation with mifepristone (MLTI) increases the spine density in developing Purkinje cells: new insights into progesterone receptor mechanisms

doi: 10.1007/s00018-013-1448-4

Figure Lengend Snippet: Morphometric analysis of progesterone effects on PC soma and dendrites. Morphometric analysis of total dendritic length (one dendrite exemplary labeled, red), total cell area and soma area (yellow) of transfected PC were done twice, before and after drug incubation for 24 h with the aid of a Zeiss LSM physiology kit. a Untreated PC (15 div) 24 h after transfection with YFP-actin (green). b The same PC after progesterone treatment for 1 day. c Changes of morphometric parameters are given in percent; error bars SD. All scale bars 50 μm

Article Snippet: Representative images of FRAP along the dendrite (ROI) at 30-s intervals. c Fluorescence intensity along the bleached region (ROI 1, red ) and in a non-bleached region (ROI 2, green ) is measured in progesterone-treated and untreated PC with the aid of the Zeiss LSM physiology kit.

Techniques: Labeling, Transfection, Incubation