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Image Search Results
Journal: Journal of medical microbiology
Article Title: Performance of the Quantamatrix Multiplexed Assay Platform system for the differentiation and identification of Mycobacterium species.
doi: 10.1099/jmm.0.000495
Figure Lengend Snippet: Fig. 2. The workflow of the Quantamatrix Multiplexed Assay Platform (QMAP) system. (a) QMAP kit, different types of shaped-disc graphically encoded carboxyl-functionalized magnetic microparticles on a glass plate. Target species genes were synthesized using a PCR process for amplification and labelled using biotin-dCTP. (b) Denaturated PCR products and a magnetic microdisc are mixed on a microwell plate, hybridized, washed and R-phycoerythrin-conjugated streptavidin-processed to produce fluorescent signals for auto- matic analysis (40 min/96 samples). (c, d) QMAP image analyser. The fluorescence intensity of all microdiscs in the image was auto- matically measured using the provided QMAP software (version 2.0) and positive fluorescence intensity signal (>500) for the identification of Mycobacterium species was auto-interpreted from Microsoft Excel program (30 s per sample).
Article Snippet: The microdiscs were then washed three times with gentle shaking in 120 μl of washing solution for 1min at 25 C, incubated at 25 C with 1 : 2000 diluted
Techniques: Synthesized, Amplification, Fluorescence, Software