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Image Search Results
Journal: Scientific Reports
Article Title: The Na + /Ca 2+ , K + exchanger 2 modulates mammalian cone phototransduction
doi: 10.1038/srep32521
Figure Lengend Snippet: ( A ) Light micrograph of retinal section from control (left) and NCKX2-deficient (right panel) mice show comparable retinal morphology. The cones, identifiable by the heterochromatic staining of cone nuclei (arrowheads) can be seen at higher magnification at the lower panel. ( B ) Whole mount staining of PNA and NCKX2 show cone-specific NCKX2 expression in the control retina (left panel) which is abolished in the Nckx2 −/− retina (right panel). ( C ) Inferior hemisphere of control (left panel) and Nckx2 −/− (right panel) retinas show a similar expression pattern of the short-wave cone opsin S-opsin. ( D ) Expression of cone-specific CNGA3 is also similar between control (left panel) and Nckx2 −/− (right panel) retinas. ( E ) Dissociated retinal cells were stained for cone marker (mCAR, cone arrestin, red) and NCKX1 (green). Scale bar = 20 μm. Both the control and the NCKX2-deficient mice were on Gnat1 −/− background which was used for subsequent cone recordings ( and ).
Article Snippet: Neuroretinas were then incubated overnight at 4 °C with the following primary antibodies: rabbit NCKX2 N2F (1:100; ref. ), mouse s-opsin 95392 (1:200; ref. ), and
Techniques: Control, Morphology, Staining, Expressing, Marker, Immunofluorescence