phosphospecific stat3 Search Results


cleaved caspase-3  (Becton Dickinson)
90
Becton Dickinson cleaved caspase-3
Cleaved Caspase 3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphospecific stat3 tyr705  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phosphospecific stat3 tyr705
Figure 1. Morphological features and <t>STAT3</t> phosphorylation of GBM-SC lines. Images of (A) matched serum-derived GBM cell lines (top) and GBM-SC lines (bottom); and (B) tumors generated from GBM-SC lines in vivo. Intracerebral transplanted tumors in GB-SCC010 and subcutaneous tumors in other cases (GB-SCC026 and 028 SC lines). Magnification, x100. (C) STAT3 phosphorylation in GBM-SC and matched serum-derived cell lines.
Phosphospecific Stat3 Tyr705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphospecific rabbit anti stat3 tyr705 antibody  (Boster Bio)
95
Boster Bio phosphospecific rabbit anti stat3 tyr705 antibody
( A ) The effects of KP on suppressing EGF-induced expression of intracellular signaling proteins determined by Western blot analysis of cell lysates from HeLa cells exposed to KP extract at different concentrations; ( B ) quantitative analysis of cellular level of interleukin (IL)-6 protein; ( C ) quantitative analysis of cellular level of GP130 protein; ( D ) quantitative analysis of signal transducers and activators of transcription 3 <t>(STAT3)</t> phosphorylation; ( E ) quantitative analysis of cellular level of Mcl-1 protein. The protein expression levels of IL-6, GP130, and Mcl1 were normalized against actin, while the phosphorylated form of STAT3 was normalized against total STAT3 protein. Data represent mean ± SD of three independent experiments. * p < 0.05 in comparison with cells treated with DMSO + EGF.
Phosphospecific Rabbit Anti Stat3 Tyr705 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphospecific stat3  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology phosphospecific stat3
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Phosphospecific Stat3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cleaved caspase-3 antibody  (Becton Dickinson)
90
Becton Dickinson cleaved caspase-3 antibody
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Cleaved Caspase 3 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal mouse anti phosphospecific stat3  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology monoclonal mouse anti phosphospecific stat3
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Monoclonal Mouse Anti Phosphospecific Stat3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphospecific polyclonal antibodies stat3 (y705  (Becton Dickinson)
90
Becton Dickinson phosphospecific polyclonal antibodies stat3 (y705
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Phosphospecific Polyclonal Antibodies Stat3 (Y705, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphospecific polyclonal antibodies stat3 (y705 - by Bioz Stars, 2026-08
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polyclonal rabbit anti phosphospecific stat 3  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc polyclonal rabbit anti phosphospecific stat 3
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Polyclonal Rabbit Anti Phosphospecific Stat 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphospecific monoclonal rabbit anti stat3 antibody  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phosphospecific monoclonal rabbit anti stat3 antibody
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Phosphospecific Monoclonal Rabbit Anti Stat3 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphospecific+stat3/pmc01525004-206-1-9?v=Cell+Signaling+Technology+Inc
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mouse monoclonal phosphospecific antibody  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc mouse monoclonal phosphospecific antibody
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Mouse Monoclonal Phosphospecific Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal phosphospecific antibody - by Bioz Stars, 2026-08
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anti phosphospeci c stat 3 antibody  (New England Biolabs)
96
New England Biolabs anti phosphospeci c stat 3 antibody
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Anti Phosphospeci C Stat 3 Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against phosphospecific stat3 (p-stat3)  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company antibodies against phosphospecific stat3 (p-stat3)
Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific <t>STAT3</t> (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h
Antibodies Against Phosphospecific Stat3 (P Stat3), supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Morphological features and STAT3 phosphorylation of GBM-SC lines. Images of (A) matched serum-derived GBM cell lines (top) and GBM-SC lines (bottom); and (B) tumors generated from GBM-SC lines in vivo. Intracerebral transplanted tumors in GB-SCC010 and subcutaneous tumors in other cases (GB-SCC026 and 028 SC lines). Magnification, x100. (C) STAT3 phosphorylation in GBM-SC and matched serum-derived cell lines.

Journal: International journal of oncology

Article Title: Effect of the STAT3 inhibitor STX-0119 on the proliferation of cancer stem-like cells derived from recurrent glioblastoma.

doi: 10.3892/ijo.2013.1916

Figure Lengend Snippet: Figure 1. Morphological features and STAT3 phosphorylation of GBM-SC lines. Images of (A) matched serum-derived GBM cell lines (top) and GBM-SC lines (bottom); and (B) tumors generated from GBM-SC lines in vivo. Intracerebral transplanted tumors in GB-SCC010 and subcutaneous tumors in other cases (GB-SCC026 and 028 SC lines). Magnification, x100. (C) STAT3 phosphorylation in GBM-SC and matched serum-derived cell lines.

Article Snippet: Antibodies against STAT3, phosphospecific STAT3 (Tyr705), cleaved caspase-3 and β-actin were purchased from Cell Signaling Technology, Inc. (Danvers, MA) and Becton-Dickinson (BD) Biosciences (Franklin Lakes, NJ) for western blotting (WB).

Techniques: Phospho-proteomics, Derivative Assay, Generated, In Vivo

Figure 3. Downregulation of STAT3 target gene expression by STX-0119 in GB-SC lines. The changes in (A) c-myc expression, (B) STAT3 target gene expression and (C) stem cell marker gene expression. (A) GB-SCC026T stem cell line was treated with STX-0119 or WP1066. (B and C) GB-SCC026 stem cell line was treated with 100 µM of STX-0119. The gene expression level without STX-0119 was rated 1 as a control and gene expression in the stem cell line is shown as fold increase. Each column shows the mean ± SD of four determinations. (A) Open column, WP1066; closed column, STX-0119. (B and C) Open column, control; closed column, STX-0119.

Journal: International journal of oncology

Article Title: Effect of the STAT3 inhibitor STX-0119 on the proliferation of cancer stem-like cells derived from recurrent glioblastoma.

doi: 10.3892/ijo.2013.1916

Figure Lengend Snippet: Figure 3. Downregulation of STAT3 target gene expression by STX-0119 in GB-SC lines. The changes in (A) c-myc expression, (B) STAT3 target gene expression and (C) stem cell marker gene expression. (A) GB-SCC026T stem cell line was treated with STX-0119 or WP1066. (B and C) GB-SCC026 stem cell line was treated with 100 µM of STX-0119. The gene expression level without STX-0119 was rated 1 as a control and gene expression in the stem cell line is shown as fold increase. Each column shows the mean ± SD of four determinations. (A) Open column, WP1066; closed column, STX-0119. (B and C) Open column, control; closed column, STX-0119.

Article Snippet: Antibodies against STAT3, phosphospecific STAT3 (Tyr705), cleaved caspase-3 and β-actin were purchased from Cell Signaling Technology, Inc. (Danvers, MA) and Becton-Dickinson (BD) Biosciences (Franklin Lakes, NJ) for western blotting (WB).

Techniques: Targeted Gene Expression, Expressing, Marker, Gene Expression, Control

( A ) The effects of KP on suppressing EGF-induced expression of intracellular signaling proteins determined by Western blot analysis of cell lysates from HeLa cells exposed to KP extract at different concentrations; ( B ) quantitative analysis of cellular level of interleukin (IL)-6 protein; ( C ) quantitative analysis of cellular level of GP130 protein; ( D ) quantitative analysis of signal transducers and activators of transcription 3 (STAT3) phosphorylation; ( E ) quantitative analysis of cellular level of Mcl-1 protein. The protein expression levels of IL-6, GP130, and Mcl1 were normalized against actin, while the phosphorylated form of STAT3 was normalized against total STAT3 protein. Data represent mean ± SD of three independent experiments. * p < 0.05 in comparison with cells treated with DMSO + EGF.

Journal: International Journal of Molecular Sciences

Article Title: Kaempferia parviflora Extract Inhibits STAT3 Activation and Interleukin-6 Production in HeLa Cervical Cancer Cells

doi: 10.3390/ijms20174226

Figure Lengend Snippet: ( A ) The effects of KP on suppressing EGF-induced expression of intracellular signaling proteins determined by Western blot analysis of cell lysates from HeLa cells exposed to KP extract at different concentrations; ( B ) quantitative analysis of cellular level of interleukin (IL)-6 protein; ( C ) quantitative analysis of cellular level of GP130 protein; ( D ) quantitative analysis of signal transducers and activators of transcription 3 (STAT3) phosphorylation; ( E ) quantitative analysis of cellular level of Mcl-1 protein. The protein expression levels of IL-6, GP130, and Mcl1 were normalized against actin, while the phosphorylated form of STAT3 was normalized against total STAT3 protein. Data represent mean ± SD of three independent experiments. * p < 0.05 in comparison with cells treated with DMSO + EGF.

Article Snippet: Primary antibodies included a 1:1000 dilution of a rabbit anti-IL-6 antibody (catalog number 12153), a rabbit anti-GP130 antibody (catalog number 3732), a mouse anti-STAT3 antibody (catalog number 9139), a rabbit anti-Mcl-1 antibody (catalog number 94296), a rabbit anti-EGFR antibody (catalog number 4267), a phosphospecific rabbit anti-STAT3 (Tyr705) antibody (catalog number 9145), phosphospecific rabbit anti-EGFR antibodies (Tyr1045 (catalog number 2237) and Tyr1068 (catalog number 3777)), a phosphospecific rabbit anti-ERK1/2 (Thr202/Tyr204) antibody (catalog number 4370), or a phosphospecific rabbit anti-Akt (Ser473) antibody (catalog number 4060), and a 1:10,000 dilution of a mouse anti-β-actin antibody (catalog number MA1115) (Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Expressing, Western Blot, Phospho-proteomics, Comparison

The effects of KP on suppressing IL-6-induced STAT3 signaling in HeLa cells. ( A ) Immunofluorescence for nuclear STAT3 phosphorylation (red) in HeLa cells treated with KP at 15 μg/mL, with the presence of human recombinant IL-6 (50 ng/mL) for 24 h. The total STAT3 protein (green) was detected to visualize total expression of STAT3; ( B ) Western blotting and quantitative analysis for pSTAT3, Mcl-1, and pAkt in HeLa cells treated with different concentrations of KP or DMSO, with the presence of human recombinant IL-6 for 24 h. Micrographs were captured at 40× magnification. Data represent mean ± SD of three replicates. * p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Kaempferia parviflora Extract Inhibits STAT3 Activation and Interleukin-6 Production in HeLa Cervical Cancer Cells

doi: 10.3390/ijms20174226

Figure Lengend Snippet: The effects of KP on suppressing IL-6-induced STAT3 signaling in HeLa cells. ( A ) Immunofluorescence for nuclear STAT3 phosphorylation (red) in HeLa cells treated with KP at 15 μg/mL, with the presence of human recombinant IL-6 (50 ng/mL) for 24 h. The total STAT3 protein (green) was detected to visualize total expression of STAT3; ( B ) Western blotting and quantitative analysis for pSTAT3, Mcl-1, and pAkt in HeLa cells treated with different concentrations of KP or DMSO, with the presence of human recombinant IL-6 for 24 h. Micrographs were captured at 40× magnification. Data represent mean ± SD of three replicates. * p < 0.05.

Article Snippet: Primary antibodies included a 1:1000 dilution of a rabbit anti-IL-6 antibody (catalog number 12153), a rabbit anti-GP130 antibody (catalog number 3732), a mouse anti-STAT3 antibody (catalog number 9139), a rabbit anti-Mcl-1 antibody (catalog number 94296), a rabbit anti-EGFR antibody (catalog number 4267), a phosphospecific rabbit anti-STAT3 (Tyr705) antibody (catalog number 9145), phosphospecific rabbit anti-EGFR antibodies (Tyr1045 (catalog number 2237) and Tyr1068 (catalog number 3777)), a phosphospecific rabbit anti-ERK1/2 (Thr202/Tyr204) antibody (catalog number 4370), or a phosphospecific rabbit anti-Akt (Ser473) antibody (catalog number 4060), and a 1:10,000 dilution of a mouse anti-β-actin antibody (catalog number MA1115) (Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Immunofluorescence, Phospho-proteomics, Recombinant, Expressing, Western Blot

Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific STAT3 (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h

Journal: Oncogene

Article Title: Role of the phosphatidylinositol 3-kinase/Akt and mTOR/P70S6-kinase pathways in the proliferation and apoptosis in multiple myeloma.

doi: 10.1038/sj.onc.1205923

Figure Lengend Snippet: Figure 5 Activation of the PI 3-K/Akt pathway in OPM2 and RPMI8226 cell lines is nort related to autocrine Il-6 production. (a) OPM2 and RPMI8226 cells were cultured at 107/ml in serum free medium for 2 h at 378C with or without LY294002 50 mM. Total cell lysates corresponding to 50 mg of proteins were sub- jected to WB analysis using anti phosphospecific STAT3 (Tyr705) or anti STAT3 antibodies. (b) OPM2 cells were cultured with an neutralizing anti-IL-6 antibody at 0.05, 0.5 or 5 mg/ml and total cells lysates were subjected to WB analysis using an anti-phosphospecific Akt (Ser 473) antibody. (c) OPM2 cells were cultured in triplicate at 105/ml in RPMI supplemented with 5% FCS for 48 h with either LY294002 at 50 mM or a neutralizing hu- man anti-IL-6 antibody at 0.05, 0.5, or 5 mg/ml. DNA synthesis was assessed by [3H]thymidine incorporation after 6 h

Article Snippet: Phosphospecific Akt (Ser473), phosphospecific P70S6K (Thr389), phosphospecific ERK-1/ERK-2, phosphospecific STAT3 and phosphospecific GSK-3ab antibodies were purchased from Cell Signaling1, Akt, Skp2, Cyclin-D1, PTEN and GSK-3a antibodies from Santa Cruz Biotechnology1, Caspase-3 and Fas-Ligand (NOK1) antibodies from Pharmingen1, phosphospecific FKHRL-1 (Thr 32) and FKHRL1 from Upstate Biotechnology1, P27Kip-1 and antiSTAT3 antibodies from Transduction Laboratories1.

Techniques: Activation Assay, Cell Culture, DNA Synthesis