Journal: Antioxidants

Article Title: Bacillus amyloliquefaciens SC06 Induced AKT–FOXO Signaling Pathway-Mediated Autophagy to Alleviate Oxidative Stress in IPEC-J2 Cells

doi: 10.3390/antiox10101545

Figure Lengend Snippet: BaSC06 mediated autophagy was triggered by the AKT–FOXO signaling pathway independent of mTOR, and the effect of BaSC06 on FOXO downstream autophagy-related target genes. ( a – c ) The expression of p-AKT, AKT, p-mTOR, and mTOR protein in IPEC-J2 cells. Cell lysates were gathered to detect the ratio of p -AKT/AKT and p-mTOR/mTOR. ( d – g ) We added 25 μM perifosine into the BaSC06 pretreated groups and collected the cell lysates to detect the protein levels of p-FOXO3/ FOXO3, LC3II/LC3I, and p62/β-actin. ( h , i ) The efficacy of all siRNAs. ( j – m ) The expression of FOXO3//β-actin, LC3II/LC3I, and p62 /β-actin in siRNA treatment groups. All data analyses were implemented using ImageJ software. Data were analyzed using one-way ANOVA with a Tukey test; n = 3 in each group; * p < 0.05, ** p < 0.01, *** p < 0.001, and ns = no significance ( p > 0.05). ( n – r ) The relative mRNA expression levels of ATG5, ATG12, ATG8, ATG14, and ATG16L1. Data were evaluated using one-way ANOVA with a Tukey test; n = 9 in each group; * p < 0.05, ** p < 0.01, and ns = no significance ( p > 0.05).

Article Snippet: Furthermore, 25 μM AKT phosphorylation inhibitor Perifosine (Biotime Biotechnology) was used to pre-incubate with IPEC-J2 cells for 48 h according to the instruction, and we then repeated the former BaSC06 and DQ treatment methods.

Techniques: Expressing, Software