Journal: ACS Omega

Article Title: Baicalein Ameliorates Experimental Ulcerative Colitis Recurrency by Downregulating Neonatal Fc Receptor via the NF-κB Signaling Pathway

doi: 10.1021/acsomega.5c00243

Figure Lengend Snippet: Effects of BCL on FcRn and related transcription factor expression and ANCA recycling in NCM460 cells. (a,b) FcRn mRNA and protein contents in NCM460 cells with gradient concentrations of BCL treatment for 48 h. Cells with a blank medium treatment were set as a control. (c) Relative recycling of ANCA in NCM460 cells after treatment with the blank medium or 40 μM BCL for 48 h. The relative recycling was calculated with the amount recycled for ANCA compared to that of the control. (d) mRNA contents of p50, p65, IκB, and STAT1 in NCM460 cells after treatment with blank medium or 40 μM BCL for 48 h. (e) Protein contents of p50, p65, IκB, pIκB, STAT1, and pSTAT1 in NCM460 cells after treatment with blank medium or 40 μM BCL for 48 h. All data are expressed as the mean ± SD ( n = 3 replicates/treatment). The protein expression of p50, p65, IκB, pIκB, STAT1, pSTAT1, and the protein/mRNA expression levels of FcRn were normalized to those of GAPDH or β-actin. n.s represents the differences that were not statistically significant ( p > 0.05). */# represent p < 0.05; ** represents p < 0.01; *** represents p < 0.001 against the control.

Article Snippet: The membrane was blocked with 5% nonfat milk and incubated with the primary antibody overnight at 4 °C, followed by incubation with the secondary antibody for 1 h. Antibodies against FcRn, p50, p65, IκB, phosphorylated IκB (pIκB), STAT1, phosphorylated STAT1 (pSTAT1), protein arginine deiminase-4 (PAD4), GAPDH, and β actin were obtained from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Control

Journal: ACS Omega

Article Title: Baicalein Ameliorates Experimental Ulcerative Colitis Recurrency by Downregulating Neonatal Fc Receptor via the NF-κB Signaling Pathway

doi: 10.1021/acsomega.5c00243

Figure Lengend Snippet: Effects of BCL on FcRn mRNA or protein expression in NCM460 cells with related transcription factors deficiency. (a–c) FcRn, p50, and p65 protein contents in NCM460 cells treated with blank medium or 40 μM BCL for 48 h in the presence or absence of p50 and p65-targeted siRNA. (d,e) FcRn and STAT1 protein contents in NCM460 cells treated with a blank medium or 40 μM BCL for 48 h in the presence or absence of STAT1-targeted siRNA. (f–h) FcRn, p50, and p65 mRNA contents in NCM460 cells treated with a blank medium or 40 μM BCL for 48 h in the presence or absence of p50 and p65-targeted siRNA. (i,j) FcRn and STAT1 mRNA contents in NCM460 cells treated with a blank medium or 40 μM BCL for 48 h in the presence or absence of STAT1-targeted siRNA. (k) FcRn mRNA contents in NCM460 cells treated with a blank medium or 40 μM BCL for 48 h in the presence or absence of p50 and p65-targeted siRNA with or without TNF-α (50 ng/mL) stimulation. (l) FcRn mRNA contents in NCM460 cells treated with a blank medium or 40 μM BCL for 48 h in the presence or absence of STAT1-targeted siRNA with or without IFN-γ (50 ng/mL) stimulation. All data are expressed as the mean ± SD ( n = 3 replicates/treatment). The mRNA or protein expression of FcRn, p50, p65, and STAT1 were normalized to those of GAPDH. Δ represents p < 0.05; **/##/ΔΔ represents p < 0.01; ***/###/ΔΔΔ represents p < 0.001; n.s represents the differences that were not statistically significant ( p > 0.05).

Article Snippet: The membrane was blocked with 5% nonfat milk and incubated with the primary antibody overnight at 4 °C, followed by incubation with the secondary antibody for 1 h. Antibodies against FcRn, p50, p65, IκB, phosphorylated IκB (pIκB), STAT1, phosphorylated STAT1 (pSTAT1), protein arginine deiminase-4 (PAD4), GAPDH, and β actin were obtained from ABclonal Technology (Wuhan, China).

Techniques: Expressing

Journal: ACS Omega

Article Title: Baicalein Ameliorates Experimental Ulcerative Colitis Recurrency by Downregulating Neonatal Fc Receptor via the NF-κB Signaling Pathway

doi: 10.1021/acsomega.5c00243

Figure Lengend Snippet: Effects of BCL on colonic FcRn and related transcription factor expression in mice with UC. (a,b) Colonic FcRn mRNA and protein contents in UC mice after SASP and BCL administration for 7 and 28 days. (c) Protein contents of colonic m-p50, m-p65, iκb, piκb, stat1, and pstat1 in UC mice after SASP and BCL administration for 28 days. All data are expressed as the mean ± SD ( n = 6 replicates/treatment). Mice administrated with UP water were used as the control (UC group). The protein expression of m-p50, m-p65, iκb, piκb, stat1, pstat1, and the protein/mRNA expression levels of FcRn were normalized to those of gapdh or β actin. * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001; n.s represents the differences that were not statistically significant ( p > 0.05).

Article Snippet: The membrane was blocked with 5% nonfat milk and incubated with the primary antibody overnight at 4 °C, followed by incubation with the secondary antibody for 1 h. Antibodies against FcRn, p50, p65, IκB, phosphorylated IκB (pIκB), STAT1, phosphorylated STAT1 (pSTAT1), protein arginine deiminase-4 (PAD4), GAPDH, and β actin were obtained from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Control