Journal: Frontiers in Microbiology

Article Title: Isoferulic acid suppresses Escherichia coli biofilm formation via LuxS/AI-2 quorum sensing inhibition and synergizes with fosfomycin

doi: 10.3389/fmicb.2026.1837128

Figure Lengend Snippet: Effects of IFA (400 μg/mL) combined with antibiotics on bacterial viability of E. coli O157:H7. (A) Fosfomycin sodium. (B) Ceftriaxone. (C) Gentamicin. (D) Polymyxin B. (E) Streptomycin. (F) Chloramphenicol. (G) Tetracycline. (H) Azithromycin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Fosfomycin sodium, cefquinome, gentamicin, polymyxin B, streptomycin, tetracycline, chloramphenicol, and azithromycin were purchased from MCE.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Isoferulic acid suppresses Escherichia coli biofilm formation via LuxS/AI-2 quorum sensing inhibition and synergizes with fosfomycin

doi: 10.3389/fmicb.2026.1837128

Figure Lengend Snippet: In vivo evaluation of the IFA/fosfomycin sodium combination strategy. (A) Mouse survival rate during the treatment period. (B) Bacterial load in ileum after different treatments. (C) Bacterial load in liver after different treatments. (D) Bacterial load in spleen after different treatments. (E) H&E staining of liver, spleen, and ileum tissues. Yellow arrows: shedding of intestinal villous epithelial cells, green arrows: cellular swelling, blue arrows: lymphocytes, purple arrows: granulocytes, black arrows: congestion, orange arrows: nuclear fragmentation, light green arrows: pigment deposition. (F) Fluorescence staining of tight junction proteins (ZO-1 and Occludin) in ileum tissues. **** p < 0.0001.

Article Snippet: Fosfomycin sodium, cefquinome, gentamicin, polymyxin B, streptomycin, tetracycline, chloramphenicol, and azithromycin were purchased from MCE.

Techniques: In Vivo, Staining, Fluorescence

Journal: Current protocols in chemical biology

Article Title: A Detailed Guide for Metabolic Incorporation of N -acetyl muramic Acid Probes into Bacterial Peptidoglycan

doi: 10.1002/cpch.74

Figure Lengend Snippet: Troubleshooting the Bacterial Cell Wall Labeling (Basic Protocol 4)

Article Snippet: Materials Stock solutions of sugars (0.1 M of NAM, 2-AzNAM, 2-AlkNAM, or 3-AzNAM in Milli-Q ® water) E. coli ΔMurQ-KU cells (see Liang, et. al 2017 2017 for strain construction; for access to strain, contact corresponding author) Petri Dishes with Clear Lid (Fisher- Catalog ID FB0875712) Isopropyl-1-thio-β-D-galactoside (IPTG) (Gold Biotechnology-Catalog ID I2481) Lysogeny broth (LB) Bacto Agar (Fisher) Chloramphenicol (34 mg/mL stock in Milli-Q ® water) (Gold Biotechnology-Catalog ID C-105) Kanamycin (50 mg/mL stock in Milli-Q® water) (Gold Biotechnology-Catalog ID K-120) Fosfomycin (10 mg/mL in Milli-Q® water) (Gold Biotechnology- Catalog ID F-470) 1 x Phosphate buffered saline (PBS) Copper sulfate (CuSO 4 ) (Fisher) Freshly prepared (+)− sodium (L) ascorbate (Fisher) Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (Sigma) Fluor 488-Alkyne (Sigma- Catalog ID61621) Poly-L-lysine (Sigma) Milli-Q ® water Cover glasses (Zeiss) 1.5 mL sterile Eppendorf tubes Prolong Diamond Antifade Mountant (Invitrogen) Sterile culture tubes 37 °C incubator Zeiss Elyra PS.1 microscope with Plan-Apochromat × 63/1.4 Oil differential interference contrast (DIC) M27 objective Plate shaker UV/Vis Spectrophotometer Tweezers Preparation of Stock Bacterial Plate 1.

Techniques: Labeling, Fluorescence, Concentration Assay, Imaging

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: A fast and sensitive LC-MS/MS method for the quantification of fosfomycin in human urine and plasma using one sample preparation method and HILIC chromatography.

doi: 10.1016/j.jchromb.2017.07.036

Figure Lengend Snippet: Fig. 1. (a) Chemical structure of fosfomycin and (b) the internal standard: fosfomycin- 13C3. Only one of the enantiomers from the racemic mixture is depicted. The three 13C atoms are marked as C*.

Article Snippet: Fosfomycin was purchased from Santa Cruz Biotechnology Inc. (Huissen, the Netherlands, purity> 98%) and racemic fosfomycin-13C3 benzylamine salt, which was used as the internal standard, was purchased from Toronto Research Chemicals (North York, Canada, purity 96%).

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: A fast and sensitive LC-MS/MS method for the quantification of fosfomycin in human urine and plasma using one sample preparation method and HILIC chromatography.

doi: 10.1016/j.jchromb.2017.07.036

Figure Lengend Snippet: Fig. 2. Chromatograms of fosfomycin in urine (a) and plasma (b) and the internal standard in urine (c) and in plasma (d) after injection of calibration standard 3.

Article Snippet: Fosfomycin was purchased from Santa Cruz Biotechnology Inc. (Huissen, the Netherlands, purity> 98%) and racemic fosfomycin-13C3 benzylamine salt, which was used as the internal standard, was purchased from Toronto Research Chemicals (North York, Canada, purity 96%).

Techniques: Clinical Proteomics, Injection