Journal: Molecular Medicine Reports

Article Title: Spinal cord stimulation reduces cardiac pain through microglial deactivation in rats with chronic myocardial ischemia

doi: 10.3892/mmr.2021.12475

Figure Lengend Snippet: Antisera used in each group.

Article Snippet: After blocking in 3% non-fat milk for 1 h at room temperature, the membranes were incubated overnight at 4°C with the following primary antibodies: Rabbit anti-p38 (1:1,000; cat. no. 8690; Cell Signaling Technology, Inc.) rabbit anti-p-p38 (1:1,000; cat. no. 4511; Cell Signaling Technology, Inc.) and rabbit anti-GAPDH (1:1,000; cat. no. 2118; Cell Signaling Technology, Inc.).

Techniques:

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Inhibition of p38 mitogen-activated protein kinase exerts a hypoglycemic effect by improving β cell function via inhibition of β cell apoptosis in db/db mice

doi: 10.1080/14756366.2018.1477138

Figure Lengend Snippet: A schematic view of the mechanisms whereby the p38 inhibitor SB203580 may reduce β cell apoptosis. ERS leads to cellular apoptosis through activation of the p38 MAPK pathway. Activation of p38 can promote the activation of ERS pathways, thus inducing apoptosis. By contrast, the p38 inhibitor SB203580 attenuates activation of the p38 MAPK pathway, reducing apoptosis mediated through the p38 pathway, but also by inhibition of ERS, as part of a benign cycle.

Article Snippet: Subsequently, proteins were blotted onto nitrocellulose membranes and probed with rabbit anti-p38 monoclonal antibody (Cell Signaling Technology, Inc.) or rabbit anti-phosphorylated p38 monoclonal antibody (Cell Signaling Technology, Inc.), followed by incubation with anti-rabbit IgG conjugated with horseradish peroxidase (ZSGB-BIO, Inc., Beijing, China).

Techniques: Activation Assay, Inhibition