Journal: BMC complementary and alternative medicine

Article Title: Dietary grape polyphenol resveratrol increases mammary tumor growth and metastasis in immunocompromised mice.

doi: 10.1186/1472-6882-13-6

Figure Lengend Snippet: Figure 5 Effect of resveratrol on Akt, JNK, and PAK1 activity in MDA-MB-231 breast cancer cells. Confluent MDA-MB-231 cells were serum starved for 24 h, treated with Veh, 0.1, 0.5, or 5 μM resveratrol (Res) for 15 min, lysed immediately, and western blotted for active and total proteins: phospho-AktThr308 and Akt, phospho-JNKThr183/Tyr185 and JNK, or phospho-PAK1Thr431/Thr402 and PAK1. Fold changes in protein activity were calculated from integrated density of positive bands and are presented relative to vehicle. (A), Representative western blots. (B), (C), and (D), Relative Akt, JNK, and PAK1 activity (phosphorylated protein/total protein), respectively (n=2-3). Error bars represent SEMs. Differences between means were determined using Student’s t-Test.

Article Snippet: Equal total protein amounts were western blotted using anti-Akt, anti-phospho AktThr308, anti-JNK, antiphospho JNKThr183/Tyr185, anti-PAK1, or anti-phospho PAK1Thr431/Thr402 (Cell Signaling Technology, Inc., Danvers, MA) antibodies.

Techniques: Activity Assay, Western Blot