phospho chk2 t68 Search Results


anti phospho chk2  (R&D Systems)
94
R&D Systems anti phospho chk2
a , IB of γH2AX, phospho(T86)- and <t>total-Chk2,</t> WRN, and GAPDH in representative cell lines after CRISPR/Cas9-mediated WRN depletion. b , γH2AX IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. c , Quantification of nuclear γH2AX staining intensity per cell. d , phospho-ATM (S1981) IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. e , IB of γH2AX, phospho(T86)- and total-Chk2, and GAPDH after RNAi-mediated WRN knockdown. f , IB of γH2AX, WRN, MLH1, MSH3, GAPDH in HCT116 with or without MMR restoration after lentiviral transduction of indicated shRNAs. g , Relative viability of HCT116 cells with and without MMR restoration 7 days after lentiviral delivery of the indicated shRNAs. Values are presented as means ± SE (n = 6). (*) p < 0.01; (**) p < 0.001; (***) p < 0.0002 by two-tailed Student’s t -test. h , WRN IF of representative cell lines. Scale bar = 20 μm i , Quantitative analysis of WRN colocalization to the nucleolar marker, fibrillarin, by Pearson’s colocalization coefficients. (*) p < 0.001; (**) p < 0.02 by two-tailed Student’s t -test.
Anti Phospho Chk2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phospho chk2/product/R&D Systems
Average 94 stars, based on 1 article reviews
anti phospho chk2 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
phospho-chk2-t68 rabbit mab  (Genecopoeia)
95
Genecopoeia phospho-chk2-t68 rabbit mab
a , IB of γH2AX, phospho(T86)- and <t>total-Chk2,</t> WRN, and GAPDH in representative cell lines after CRISPR/Cas9-mediated WRN depletion. b , γH2AX IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. c , Quantification of nuclear γH2AX staining intensity per cell. d , phospho-ATM (S1981) IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. e , IB of γH2AX, phospho(T86)- and total-Chk2, and GAPDH after RNAi-mediated WRN knockdown. f , IB of γH2AX, WRN, MLH1, MSH3, GAPDH in HCT116 with or without MMR restoration after lentiviral transduction of indicated shRNAs. g , Relative viability of HCT116 cells with and without MMR restoration 7 days after lentiviral delivery of the indicated shRNAs. Values are presented as means ± SE (n = 6). (*) p < 0.01; (**) p < 0.001; (***) p < 0.0002 by two-tailed Student’s t -test. h , WRN IF of representative cell lines. Scale bar = 20 μm i , Quantitative analysis of WRN colocalization to the nucleolar marker, fibrillarin, by Pearson’s colocalization coefficients. (*) p < 0.001; (**) p < 0.02 by two-tailed Student’s t -test.
Phospho Chk2 T68 Rabbit Mab, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho-chk2-t68 rabbit mab/product/Genecopoeia
Average 95 stars, based on 1 article reviews
phospho-chk2-t68 rabbit mab - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
ptp1b assay  (R&D Systems)
90
R&D Systems ptp1b assay
a , IB of γH2AX, phospho(T86)- and <t>total-Chk2,</t> WRN, and GAPDH in representative cell lines after CRISPR/Cas9-mediated WRN depletion. b , γH2AX IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. c , Quantification of nuclear γH2AX staining intensity per cell. d , phospho-ATM (S1981) IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. e , IB of γH2AX, phospho(T86)- and total-Chk2, and GAPDH after RNAi-mediated WRN knockdown. f , IB of γH2AX, WRN, MLH1, MSH3, GAPDH in HCT116 with or without MMR restoration after lentiviral transduction of indicated shRNAs. g , Relative viability of HCT116 cells with and without MMR restoration 7 days after lentiviral delivery of the indicated shRNAs. Values are presented as means ± SE (n = 6). (*) p < 0.01; (**) p < 0.001; (***) p < 0.0002 by two-tailed Student’s t -test. h , WRN IF of representative cell lines. Scale bar = 20 μm i , Quantitative analysis of WRN colocalization to the nucleolar marker, fibrillarin, by Pearson’s colocalization coefficients. (*) p < 0.001; (**) p < 0.02 by two-tailed Student’s t -test.
Ptp1b Assay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ptp1b assay/product/R&D Systems
Average 90 stars, based on 1 article reviews
ptp1b assay - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
pchk2  (Boster Bio)
92
Boster Bio pchk2
a , IB of γH2AX, phospho(T86)- and <t>total-Chk2,</t> WRN, and GAPDH in representative cell lines after CRISPR/Cas9-mediated WRN depletion. b , γH2AX IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. c , Quantification of nuclear γH2AX staining intensity per cell. d , phospho-ATM (S1981) IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. e , IB of γH2AX, phospho(T86)- and total-Chk2, and GAPDH after RNAi-mediated WRN knockdown. f , IB of γH2AX, WRN, MLH1, MSH3, GAPDH in HCT116 with or without MMR restoration after lentiviral transduction of indicated shRNAs. g , Relative viability of HCT116 cells with and without MMR restoration 7 days after lentiviral delivery of the indicated shRNAs. Values are presented as means ± SE (n = 6). (*) p < 0.01; (**) p < 0.001; (***) p < 0.0002 by two-tailed Student’s t -test. h , WRN IF of representative cell lines. Scale bar = 20 μm i , Quantitative analysis of WRN colocalization to the nucleolar marker, fibrillarin, by Pearson’s colocalization coefficients. (*) p < 0.001; (**) p < 0.02 by two-tailed Student’s t -test.
Pchk2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pchk2/product/Boster Bio
Average 92 stars, based on 1 article reviews
pchk2 - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
anti p t68 chk2  (R&D Systems)
90
R&D Systems anti p t68 chk2
A . Quantification of CellTiterBlue (CTB) viability assay in human A549 and H441 NSCLC cell lines treated with vehicle, STA-8666 and irinotecan in doses of 0.1, 1, 10 μM for up to 5 days. Chart curves represent average data from two independent runs performed with duplicate samples. B-E . Western blot analysis of phosphorylated (p) and total (t) ERK1/2 ( B ), AKT ( C ), <t>CHK2</t> ( D ) and CDK1 ( E ) protein expression in human A549 and H441 NSCLC cell lines, at 48 hours after treatment with vehicle (V), STA-8666 and irinotecan in doses of 0.1, 1, or 10 μM, as indicated. Histograms represent data from two independent experiments. All graphs: *, p≤0.05; **, p≤0.01; ***, p≤0.001 relative to vehicle controls.
Anti P T68 Chk2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p t68 chk2/product/R&D Systems
Average 90 stars, based on 1 article reviews
anti p t68 chk2 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti phospho chk2 t68 r d systems af1626  (R&D Systems)
93
R&D Systems anti phospho chk2 t68 r d systems af1626
A . Quantification of CellTiterBlue (CTB) viability assay in human A549 and H441 NSCLC cell lines treated with vehicle, STA-8666 and irinotecan in doses of 0.1, 1, 10 μM for up to 5 days. Chart curves represent average data from two independent runs performed with duplicate samples. B-E . Western blot analysis of phosphorylated (p) and total (t) ERK1/2 ( B ), AKT ( C ), <t>CHK2</t> ( D ) and CDK1 ( E ) protein expression in human A549 and H441 NSCLC cell lines, at 48 hours after treatment with vehicle (V), STA-8666 and irinotecan in doses of 0.1, 1, or 10 μM, as indicated. Histograms represent data from two independent experiments. All graphs: *, p≤0.05; **, p≤0.01; ***, p≤0.001 relative to vehicle controls.
Anti Phospho Chk2 T68 R D Systems Af1626, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phospho chk2 t68 r d systems af1626/product/R&D Systems
Average 93 stars, based on 1 article reviews
anti phospho chk2 t68 r d systems af1626 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


a , IB of γH2AX, phospho(T86)- and total-Chk2, WRN, and GAPDH in representative cell lines after CRISPR/Cas9-mediated WRN depletion. b , γH2AX IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. c , Quantification of nuclear γH2AX staining intensity per cell. d , phospho-ATM (S1981) IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. e , IB of γH2AX, phospho(T86)- and total-Chk2, and GAPDH after RNAi-mediated WRN knockdown. f , IB of γH2AX, WRN, MLH1, MSH3, GAPDH in HCT116 with or without MMR restoration after lentiviral transduction of indicated shRNAs. g , Relative viability of HCT116 cells with and without MMR restoration 7 days after lentiviral delivery of the indicated shRNAs. Values are presented as means ± SE (n = 6). (*) p < 0.01; (**) p < 0.001; (***) p < 0.0002 by two-tailed Student’s t -test. h , WRN IF of representative cell lines. Scale bar = 20 μm i , Quantitative analysis of WRN colocalization to the nucleolar marker, fibrillarin, by Pearson’s colocalization coefficients. (*) p < 0.001; (**) p < 0.02 by two-tailed Student’s t -test.

Journal: bioRxiv

Article Title: WRN Helicase is a Synthetic Lethal Target in Microsatellite Unstable Cancers

doi: 10.1101/502070

Figure Lengend Snippet: a , IB of γH2AX, phospho(T86)- and total-Chk2, WRN, and GAPDH in representative cell lines after CRISPR/Cas9-mediated WRN depletion. b , γH2AX IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. c , Quantification of nuclear γH2AX staining intensity per cell. d , phospho-ATM (S1981) IF of representative cell lines after delivery of indicated sgRNAs. Scale bar = 50 μm. e , IB of γH2AX, phospho(T86)- and total-Chk2, and GAPDH after RNAi-mediated WRN knockdown. f , IB of γH2AX, WRN, MLH1, MSH3, GAPDH in HCT116 with or without MMR restoration after lentiviral transduction of indicated shRNAs. g , Relative viability of HCT116 cells with and without MMR restoration 7 days after lentiviral delivery of the indicated shRNAs. Values are presented as means ± SE (n = 6). (*) p < 0.01; (**) p < 0.001; (***) p < 0.0002 by two-tailed Student’s t -test. h , WRN IF of representative cell lines. Scale bar = 20 μm i , Quantitative analysis of WRN colocalization to the nucleolar marker, fibrillarin, by Pearson’s colocalization coefficients. (*) p < 0.001; (**) p < 0.02 by two-tailed Student’s t -test.

Article Snippet: Types of primary antibodies and the dilutions used for immunofluorescence were as follows: anti-γH2AX (Millipore Sigma, 05-636, 1:400); anti-p21 (Santa Cruz Biotechnology, sc-6246, 1:100); anti-phospho ATM [S1981] (Millipore Sigma, 05-740, 1:200); anti-phospho Chk2 [T68] (R&D Systems, AF1626, 1:100); anti-fibrillarin (Abcam, ab5821, 1:500); anti-phospho p53 [S15] (Cell Signaling Technology, 9284, 1:100); anti-WRN (Sigma W0393, 1:200).

Techniques: CRISPR, Staining, Transduction, Two Tailed Test, Marker

A . Quantification of CellTiterBlue (CTB) viability assay in human A549 and H441 NSCLC cell lines treated with vehicle, STA-8666 and irinotecan in doses of 0.1, 1, 10 μM for up to 5 days. Chart curves represent average data from two independent runs performed with duplicate samples. B-E . Western blot analysis of phosphorylated (p) and total (t) ERK1/2 ( B ), AKT ( C ), CHK2 ( D ) and CDK1 ( E ) protein expression in human A549 and H441 NSCLC cell lines, at 48 hours after treatment with vehicle (V), STA-8666 and irinotecan in doses of 0.1, 1, or 10 μM, as indicated. Histograms represent data from two independent experiments. All graphs: *, p≤0.05; **, p≤0.01; ***, p≤0.001 relative to vehicle controls.

Journal: PLoS ONE

Article Title: Tumor-targeted SN38 inhibits growth of early stage non-small cell lung cancer (NSCLC) in a KRas/p53 transgenic mouse model

doi: 10.1371/journal.pone.0176747

Figure Lengend Snippet: A . Quantification of CellTiterBlue (CTB) viability assay in human A549 and H441 NSCLC cell lines treated with vehicle, STA-8666 and irinotecan in doses of 0.1, 1, 10 μM for up to 5 days. Chart curves represent average data from two independent runs performed with duplicate samples. B-E . Western blot analysis of phosphorylated (p) and total (t) ERK1/2 ( B ), AKT ( C ), CHK2 ( D ) and CDK1 ( E ) protein expression in human A549 and H441 NSCLC cell lines, at 48 hours after treatment with vehicle (V), STA-8666 and irinotecan in doses of 0.1, 1, or 10 μM, as indicated. Histograms represent data from two independent experiments. All graphs: *, p≤0.05; **, p≤0.01; ***, p≤0.001 relative to vehicle controls.

Article Snippet: Primary antibodies were used at a 1:1000 dilution and included: anti-PARP (#9542L Cell Signaling, Beverly, MA), anti-pT 202 /Y 204 -ERK1/2 (#4996S Cell Signaling, Beverly, MA), anti-Erk1/2, (#4696S Cell Signaling, Beverly, MA), anti-p T308 Akt, (#2965S Cell Signaling, Beverly, MA), anti-Akt, (#2920S Cell Signaling, Beverly, MA), anti-p T68 Chk2, (#MAB1626 R&D Systems, Minneapolis, MN); anti-Chk2, (#3440 Cell Signaling, Beverly, MA), anti-p Y15 CDK1, (#9111L Cell Signaling, Beverly, MA), anti-CDK1, (#SC54 Santa Cruz Biotechnology, Dallas, TX); anti-E-cadherin, (#3195S Cell Signaling, Beverly, MA); anti-vimentin, (ab92547, Abcam, Cambridge, MA), anti-Snail, (ab180714, Abcam, Cambridge, MA), and anti-vinculin (mouse, monoclonal hVIN-1, #V9131, Sigma-Aldrich, St. Louis, MO).

Techniques: Viability Assay, Western Blot, Expressing