Journal: Bioactive Materials

Article Title: An axolotl limb regeneration-inspired strategy to enhance alveolar bone regeneration

doi: 10.1016/j.bioactmat.2025.02.020

Figure Lengend Snippet: Prrx1 + stem cells demonstrated a critical role in BMP-2-induced osteogenesis . ( A ) Schematic illustration of a mouse distraction osteogenesis model. ( B ) Genes of osteogenic stem cells sorted by time of peak expression during jaw regeneration.( C ) Expression patterns of Bmpr1a and Prrx1 . ( D ) Co-localization of Prrx1 and BMPR1A in hPDLSCs. ( E ) Schematic diagram of cell migration experiment using a three-channel microfluidic chip. ( F ) After hPDLSCs adhered in the central channel, culture medium containing 200 ng/mL BMP-2 was perfused in the upper channel while the standard culture medium in lower channel; cell migration is observed after 8 h. ( G ) Schematic of semi-open silk sponges subcutaneously implanted with open sides facing either the epithelial or muscular region in Mice. ( H ) Sp7/ALP co-staining was performed to assess osteogenic effects in the localized region on day 7. ( I ) Schematic of silk sponges for dorsal subcutaneous transplantation in Prrx1-cre; R26R tdTomato mice. ( J ) ALP immunofluorescence staining was utilized to trace the osteogenic differentiation of BMP-2-recruited Prrx1 + stem cells. n = 3 biological replicates. Illustration created with BioRender.

Article Snippet: In immunofluorescence staining procedure, the cells or decalcified sections were incubated overnight at 4 °C with primary antibodies: Prrx1 (ab211292, Abcam, USA), BMPR1A (ab264043, Abcam, USA), α-SMA (ab124964, Abcam, USA),CD31 (AF3628, R&D systems, USA), MyD88 (ab2064, Abcam, USA), ALP (AF2910, R&D systems, USA), Sp7 (ab22552, Abcam, USA) and OCN (23418-1-AP, Proteintech, China).

Techniques: Expressing, Migration, Staining, Transplantation Assay, Immunofluorescence

Journal: Bioactive Materials

Article Title: An axolotl limb regeneration-inspired strategy to enhance alveolar bone regeneration

doi: 10.1016/j.bioactmat.2025.02.020

Figure Lengend Snippet: Channel structure and BMP-2 induced ectopic recruitment of Prrx1 + stem cells for osteogenesis . ( A ) Schematic illustration of the single-channel PDMS film for dorsal subcutaneous transplantation in rats. ( B ) Evaluation of tissue infiltration and collagen formation within the channel structure using HE staining and Masson's trichrome staining on days 4 and 7. ( C ) Prrx1/ALP co-staining detects the osteogenesis of BMP-2 ectopically recruited Prrx1 + stem cells on day 7. ( D and E ) SP7 (D) and OCN (E) immunofluorescence staining for ectopic osteogenesis within channel structure on day 7. n = 4 biological replicates. Illustration created with BioRender.

Article Snippet: In immunofluorescence staining procedure, the cells or decalcified sections were incubated overnight at 4 °C with primary antibodies: Prrx1 (ab211292, Abcam, USA), BMPR1A (ab264043, Abcam, USA), α-SMA (ab124964, Abcam, USA),CD31 (AF3628, R&D systems, USA), MyD88 (ab2064, Abcam, USA), ALP (AF2910, R&D systems, USA), Sp7 (ab22552, Abcam, USA) and OCN (23418-1-AP, Proteintech, China).

Techniques: Transplantation Assay, Staining, Immunofluorescence

Journal: Bioactive Materials

Article Title: An axolotl limb regeneration-inspired strategy to enhance alveolar bone regeneration

doi: 10.1016/j.bioactmat.2025.02.020

Figure Lengend Snippet: Synergistic osteogenic effects of BMP-2 and channel structure in PDMS cube and subgingival tissue . ( A ) Schematic illustration showing the PDMS cube implanted subcutaneously in rats as a BCS model. ( B ) Evaluation of collagen formation within the PDMS cube via Masson's trichrome staining on day 14. ( C ) Assessment of the sequential induction of stem cell infiltration and osteogenesis by BMP-2 and channel structures through Prrx1/ALP co-staining on day 14. ( D to F ) Whole-sample scan of CD31 staining on day 14 showing overall vascularization within the PDMS cubes (D), along with magnified views of the channel region (E) and the central region (F). ( G ) Schematic illustration of the single-channel PDMS film for subgingival transplantation in rats. ( H ) HE staining and Masson's trichrome staining were used to assess tissue infiltration and collagen formation within channel structures on days 4 and 7. ( I ) Prrx1 and OCN immunofluorescence staining for ectopic osteogenesis within channel structure on day 7. n = 4 biological replicates. Illustration created with BioRender.

Article Snippet: In immunofluorescence staining procedure, the cells or decalcified sections were incubated overnight at 4 °C with primary antibodies: Prrx1 (ab211292, Abcam, USA), BMPR1A (ab264043, Abcam, USA), α-SMA (ab124964, Abcam, USA),CD31 (AF3628, R&D systems, USA), MyD88 (ab2064, Abcam, USA), ALP (AF2910, R&D systems, USA), Sp7 (ab22552, Abcam, USA) and OCN (23418-1-AP, Proteintech, China).

Techniques: Staining, Transplantation Assay, Immunofluorescence