Journal: Plant Physiology

Article Title: Evidence for a Role of Chloroplastic m-Type Thioredoxins in the Biogenesis of Photosystem II in Arabidopsis ^{1 [C] [W] [OPEN]}

doi: 10.1104/pp.113.228353

Figure Lengend Snippet: Immunoblot analyses of thylakoid proteins from VIGS plants. A, Immunoblot analysis of thylakoid proteins loaded on the basis of equal total thylakoid proteins. Aliquots of 2.5 μg (¼ VIGS-GFP), 5 μg (½ VIGS-GFP), 10 μg (VIGS-GFP), and 10 μg (VIGS-TRX m) of total thylakoid proteins were loaded on the gels. Designations of thylakoid membrane protein complexes and their diagnostic components are labeled on the left. Three biological replicates were performed, and similar results were obtained. B, Semiquantitative analysis of thylakoid proteins. Immunoblots in A from three biological replicates were analyzed with Phoretix 1D software (Phoretix International). The protein contents (per unit of thylakoid protein) of the thylakoid membranes from VIGS plants were normalized to the level of the β-subunit of the ATP synthase (CF1 β). Protein levels in the VIGS-TRX m plants are shown relative to the levels in the VIGS-GFP leaves (100%). Data are given as means ± sd of three biological replicates.

Article Snippet: Immunoblots in A from three biological replicates were analyzed with Phoretix 1D software (Phoretix International).

Techniques: Western Blot, Diagnostic Assay, Labeling, Software