Journal: Cancers

Article Title: Resveratrol and AG490 Overcome Glioblastoma Cells’ Resistance to Monotherapy by Inhibiting JAK2/STAT3 Signalling Pathway

doi: 10.3390/cancers18050794

Figure Lengend Snippet: RES and AG490 suppress STAT3 signaling pathway activation in GBM cells. ( a ) Representative immunofluorescence images showing pSTAT3 (green) and nuclear staining with Hoechst (blue) in LN428 and U251 cells treated with RES, AG490, or RES + AG490. Merged images display the overlay of pSTAT3 and nuclear signals. ( b ) Representative immunocytochemistry results demonstrating pSTAT3 protein expression. ( c ) Representative Western blot results showing total STAT3 and pSTAT3 protein expression in both cells treated with RES, AG490, and RES + AG490. GAPDH serves as the loading control. ( d ) Densitometric quantification of STAT3 and pSTAT3 protein expression levels normalized to GAPDH in LN428 and U251 cells. Data are presented as mean ± SD from three independent experiments. Statistical significance was determined using one-way ANOVA followed by Dunnett’s post hoc test. * p < 0.05, ** p < 0.01, **** p < 0.0001. Scale bar: 100 µm.

Article Snippet: Afterword, the membranes were blocked with 5% skim milk for 2 h, and washed thrice with Tris-buffered saline (TBS-T, 8 min each), and incubated overnight at 4 °C with primary antibodies, Rabbit polyclonal anti STAT3 (1:1000, Protein Tech, Rosemont, IL, USA 10253-2-AP), Rabbit polyclonal anti pSTAT3 (1:1000, abs118973), Rabbit polyclonal anti BAX (1:1000, Protein Tech, USA 50599-2-lg), Rabbit polyclonal anti BCL-2 (1:1000, Protein Tech, USA 26593-1-AP), and Rabbit polyclonal anti- GAPDH (1:5000, Proteintech, Wuhan, China 10494-1-AP).

Techniques: Activation Assay, Immunofluorescence, Staining, Immunocytochemistry, Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis

doi: 10.1038/s41598-026-36351-2

Figure Lengend Snippet: The suppressed levels of CDC25C in cell line and xenograft model. ( A ) The CDC25C gene was detected via agarose gel electrophoresis, with β-actin serving as an internal control. The marker used was a 1000 bp Gene Ruler, and the expected product sizes were 970 bp for CDC25C and 709 bp for β-actin. The negative control prepared from a sample containing just the Master-Mix, in which there was no DNA contamination. ( B ) The relative expression of CDC25C mRNA was evaluated using qRT-PCR. ( C ) The expression level of CDC25C protein was assessed through Western blotting and quantified using ImageJ software, with GAPDH serving as the internal control. Data are presented as means ± SDs from three independent experiments. c p <0.001.

Article Snippet: CDC25C , Mouse , #66912-1-Ig , Proteintech , , 1:1000.

Techniques: Agarose Gel Electrophoresis, Control, Marker, Negative Control, Expressing, Quantitative RT-PCR, Western Blot, Software

Journal: Scientific Reports

Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis

doi: 10.1038/s41598-026-36351-2

Figure Lengend Snippet: Downregulation of CDC25C inhibited the malignant biological behaviors of Hepa1-6 cells. ( A ) The colony formation ability was assessed using a plate cloning assay at 100× magnification, with the colony formation rate calculated by counting the number of colonies containing more than 50 cells. ( B ) The lateral migration ability of cells was measured using a wound healing assay at 100× magnification, and the migration rate (scratch healing rate) was calculated by measuring the width of the scratch. ( C , D ) The longitudinal migration and invasion abilities of the cells were evaluated using Transwell assays, quantifying the number of cells that passed through the filter. The scale bar represents 20 μm. Data are presented as means ± SDs from three independent experiments. a p <0.05, b p <0.01, c p <0.001.

Article Snippet: CDC25C , Mouse , #66912-1-Ig , Proteintech , , 1:1000.

Techniques: Cloning, Migration, Wound Healing Assay

Journal: Scientific Reports

Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis

doi: 10.1038/s41598-026-36351-2

Figure Lengend Snippet: Downregulation of CDC25C altered the morphology of the subcellular structure of Hepa1-6 cells. The images of Hepa1-6 cells captured under transmission electron microscopy. The scale bar represents a measurement of 2 μm at lower magnification and 200 nm at higher magnification. White arrows indicate healthy mitochondria, orange arrows denote autophagosomes, blue arrow highlight cellular vacuolation, and red arrows signify swollen and dysfunctional mitochondria.

Article Snippet: CDC25C , Mouse , #66912-1-Ig , Proteintech , , 1:1000.

Techniques: Transmission Assay, Electron Microscopy

Journal: Scientific Reports

Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis

doi: 10.1038/s41598-026-36351-2

Figure Lengend Snippet: Downregulation of CDC25C induced a mitochondrial stress response. ( A , B ) Mitochondrial calcium concentrations were measured in Hepa1-6 and AML12 cells using the Rhod-2/AM fluorescent probe, with ImageJ software employed to quantify the red fluorescence intensity. ( C , D ) ROS levels were assessed in Hepa1-6 cells and AML12 using the MitoSOX fluorescent probe, and the red fluorescence intensity was quantified using ImageJ software. ( E ) The relative mRNA expression levels of CHOP, HSP60, ClpP, and LONP1 were evaluated via qRT-PCR. ( F , G ) The relative protein expression levels of CHOP, HSP60, ClpP, and LONP1 were determined through Western blotting and quantified using ImageJ software, with GAPDH serving as the internal control. The scale bar represents 10 μm. Data are presented as means ± SDs from three independent experiments. a p <0.05, b p <0.01, c p <0.001. ‘ns’ indicates no statistical significance.

Article Snippet: CDC25C , Mouse , #66912-1-Ig , Proteintech , , 1:1000.

Techniques: Software, Fluorescence, Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Scientific Reports

Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis

doi: 10.1038/s41598-026-36351-2

Figure Lengend Snippet: Downregulation of CDC25C induced an autophagic response. ( A ) The relative mRNA expression levels of LC3, p62, and Beclin1 were evaluated using qRT-PCR. ( B , C ) The relative protein expression levels of LC3, p62, and Beclin1 were determined through Western blotting and quantified using ImageJ software, with GAPDH serving as the internal control. Data are presented as means ± SDs from three independent experiments. a p < 0.05, b p < 0.01, c p <0.001.

Article Snippet: CDC25C , Mouse , #66912-1-Ig , Proteintech , , 1:1000.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Software, Control

Journal: Scientific Reports

Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis

doi: 10.1038/s41598-026-36351-2

Figure Lengend Snippet: Downregulation of CDC25C induced a mitochondria-mediated apoptosis. ( A ) Hoechst 33258 staining was employed to morphologically identify apoptotic cells. ( B ) Annexin V/TMRE costaining was utilized for apoptosis detection via flow cytometry to investigate the apoptosis rate and quantified using ImageJ software. ( C ) The relative mRNA expression levels of Cyt c, Caspase-3 and Caspase-9 were evaluated using qRT-PCR. ( D , E ) The relative protein expression levels of Cyt c, Caspase-3 and Caspase-9 were determined through Western blotting and quantified using ImageJ software, with GAPDH serving as the internal control. The scale bar represents 50 μm. Data are presented as means ± SDs from three independent experiments. a p < 0.05, b p < 0.01, c p <0.001. ‘ns’ indicates no statistical significance.

Article Snippet: CDC25C , Mouse , #66912-1-Ig , Proteintech , , 1:1000.

Techniques: Staining, Flow Cytometry, Software, Expressing, Quantitative RT-PCR, Western Blot, Control