U73122 PLC
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2021-03
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![Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor U73122 (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.](https://storage.googleapis.com/bioz_article_images/PMC4720552/JNC-136-550-g003.jpg)
Journal: Journal of Neurochemistry
Article Title: Functional interplay between ganglioside GM1 and cross‐linking galectin‐1 induces axon‐like neuritogenesis via integrin‐based signaling and TRPC5‐dependent Ca2+ influx
doi: 10.1111/jnc.13418
Figure Lengend Snippet: Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor U73122 (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.
Article Snippet: For testing involvement of PLC, PI(3)K and TRPC5 channels in Gal‐1‐induced neuritogenesis,
Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Staining, Inhibition, Planar Chromatography