Journal: PLoS ONE

Article Title: Disruption of Hydrogen Bonds between Major Histocompatibility Complex Class II and the Peptide N-Terminus Is Not Sufficient to Form a Human Leukocyte Antigen-DM Receptive State of Major Histocompatibility Complex Class II

doi: 10.1371/journal.pone.0069228

Figure Lengend Snippet: (A–D) Overlay of DR1 carrying a truncated HA peptide variant (pHA* P1-P11 [P1V] ) missing N-terminal residues P-2 and P-1 (DRα cyan, DR1β blue, peptide green) and DR1 carrying full-length HA peptide (pHA P-2-P11 , gray, 1DLH). (A) Side view of overlaid DR1 ectodomains. (B) Top view of overlaid peptide-binding grooves with side chains near the peptide N-terminus shown in stick representation. (C) Close-up view of selected DRα chain residues shown in stick model near the P1 anchor residue. (D) Close-up view of selected DR1β chain residues shown in stick model near the P1 anchor.

Article Snippet: This peptide and its unlabeled counterpart pHA P-2-P11 PKYVKQNTLKLAT were also ordered from Peptide 2.0 (Chantilly, VA, USA).

Techniques: Variant Assay, Binding Assay

Journal: PLoS ONE

Article Title: Disruption of Hydrogen Bonds between Major Histocompatibility Complex Class II and the Peptide N-Terminus Is Not Sufficient to Form a Human Leukocyte Antigen-DM Receptive State of Major Histocompatibility Complex Class II

doi: 10.1371/journal.pone.0069228

Figure Lengend Snippet: (A) Top view of peptide-binding groove with pHA* P1-P11 [P1V] linked at αC65 (DRα, ribbon, cyan; DR1β, ribbon, blue; peptide, stick, green). (B) Close-up view of the peptide N-terminus of the structure shown in (A). Hydrogen bonds between DR1 residues and the peptide N-terminus are shown with dotted lines. A water molecule coordinating a hydrogen bond from DR1β H81 to the peptide N-terminus is shown as a blue sphere. (C) Top view of peptide-binding groove with full-length pHA P-2-P11 (ribbon and stick, gray; 1DLH). (D) Close-up view of the peptide N-terminus of the structure shown in (C). Hydrogen bonds between DR1 residues and the peptide N-terminus are shown with dotted lines. (E) Superimposition of truncated (green) and full-length (gray) peptide.

Article Snippet: This peptide and its unlabeled counterpart pHA P-2-P11 PKYVKQNTLKLAT were also ordered from Peptide 2.0 (Chantilly, VA, USA).

Techniques: Binding Assay

Journal: PLoS ONE

Article Title: Disruption of Hydrogen Bonds between Major Histocompatibility Complex Class II and the Peptide N-Terminus Is Not Sufficient to Form a Human Leukocyte Antigen-DM Receptive State of Major Histocompatibility Complex Class II

doi: 10.1371/journal.pone.0069228

Figure Lengend Snippet: (A, B) DR1β mutants H81A, V85S and V85D and DRα mutant S53A were compared with wild type DR1 complexes in peptide dissociation assays. Dissociation at pH 5.3 and 25 o C of Alexa-488-labeled full-length pHA P-2-P11 [P5Alexa] peptide from preloaded DR1 complexes (100 nM) in the presence of 50 µM unlabeled pHA P-2-P11 competitor peptide was measured by FP. Dissociation curves were preformed in the absence (A) or presence (B) of 100 nM DM. FP values are presented in millipolarization units (mP).

Article Snippet: This peptide and its unlabeled counterpart pHA P-2-P11 PKYVKQNTLKLAT were also ordered from Peptide 2.0 (Chantilly, VA, USA).

Techniques: Mutagenesis, Labeling

Journal: PLoS ONE

Article Title: Disruption of Hydrogen Bonds between Major Histocompatibility Complex Class II and the Peptide N-Terminus Is Not Sufficient to Form a Human Leukocyte Antigen-DM Receptive State of Major Histocompatibility Complex Class II

doi: 10.1371/journal.pone.0069228

Figure Lengend Snippet: Peptide abbreviations.

Article Snippet: This peptide and its unlabeled counterpart pHA P-2-P11 PKYVKQNTLKLAT were also ordered from Peptide 2.0 (Chantilly, VA, USA).

Techniques: