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Image Search Results
Journal: Cells
Article Title: Pseudohypoxia-Stabilized HIF2α Transcriptionally Inhibits MNRR1 , a Druggable Target in MELAS
doi: 10.3390/cells14141078
Figure Lengend Snippet: PHD3 levels are reduced in MELAS cybrid cells and enhanced by nitazoxanide to increase MNRR1 levels. ( A ): Transcript levels of PHD1 , 2 , and 3 in MELAS cybrid cells harboring the levels of heteroplasmy shown (data from ). ( B ): Protein levels of PHD3 in MELAS cybrid cells with 0% (CL9) and 73% heteroplasmy (DW7). GAPDH was probed as loading control. ( C ): Equal amounts of MELAS cybrid cell lysates, treated with vehicle (DMSO), nitazoxanide, or tizoxanide (10 μM) for 24 h, were separated on an SDS-PAGE gel and probed for PHD1, PHD3, MNRR1, and GAPDH. ( D ): Oxygen consumption measured from MELAS cybrid cells overexpressing PHD1 or PHD3 (n = 4 biological replicates, error bars show SE). ** indicates p < 0.005.
Article Snippet: The MNRR1 (19424-1), MTCO2 (55070-1), PHD1 (12984-1), and
Techniques: Control, SDS Page