pgrna Search Results


lentiviral u6  (Addgene inc)
94
Addgene inc lentiviral u6
(A) Impact of overexpression of atypical E2F proteins on cell cycle in WT T cells. WT T cells were infected with E2F7/E2F8 <t>lentiviral</t> particles, treated with anti-CD3 and anti-CD28 Abs, and analyzed for cell cycle. Combined data were from 3 independent experiments. (B) Overexpression of E2F7/E2F8 significantly inhibited T cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (C) Impact of E2F7/E2F8 silencing on cell cycle in miR-142 KO T cells. miR-142 KO T cells were targeted for E2F7/E2F8 silencing as described in Methods, then treated with anti-CD3 and anti-CD28 Abs for 0 to 3 days. Cell-cycle analyses were processed. Combined data were from 3 independent experiments. (D) KD of E2F7/E2F8 in miR-142 KO T cells significantly improved cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (E) Essential role of E2F7/E2F8 in cell-cycle activities in WT T cells. WT T cells were targeted for E2F7/E2F8 silencing, treated with anti-CD3 and anti-CD28 Abs for 0 to 4 days, and processed for cell-cycle analyses as in C. Data were combined from 3 independent experiments. (F) KD of E2F7/E2F8 in WT T cells significantly enhanced cell proliferation as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01, Holm-Sidak method (A, C, and E); unpaired t test (B, D, and F).
Lentiviral U6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgrna  (Addgene inc)
93
Addgene inc pgrna
(A) Impact of overexpression of atypical E2F proteins on cell cycle in WT T cells. WT T cells were infected with E2F7/E2F8 <t>lentiviral</t> particles, treated with anti-CD3 and anti-CD28 Abs, and analyzed for cell cycle. Combined data were from 3 independent experiments. (B) Overexpression of E2F7/E2F8 significantly inhibited T cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (C) Impact of E2F7/E2F8 silencing on cell cycle in miR-142 KO T cells. miR-142 KO T cells were targeted for E2F7/E2F8 silencing as described in Methods, then treated with anti-CD3 and anti-CD28 Abs for 0 to 3 days. Cell-cycle analyses were processed. Combined data were from 3 independent experiments. (D) KD of E2F7/E2F8 in miR-142 KO T cells significantly improved cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (E) Essential role of E2F7/E2F8 in cell-cycle activities in WT T cells. WT T cells were targeted for E2F7/E2F8 silencing, treated with anti-CD3 and anti-CD28 Abs for 0 to 4 days, and processed for cell-cycle analyses as in C. Data were combined from 3 independent experiments. (F) KD of E2F7/E2F8 in WT T cells significantly enhanced cell proliferation as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01, Holm-Sidak method (A, C, and E); unpaired t test (B, D, and F).
Pgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgrna ckb  (Addgene inc)
93
Addgene inc pgrna ckb
(A) Impact of overexpression of atypical E2F proteins on cell cycle in WT T cells. WT T cells were infected with E2F7/E2F8 <t>lentiviral</t> particles, treated with anti-CD3 and anti-CD28 Abs, and analyzed for cell cycle. Combined data were from 3 independent experiments. (B) Overexpression of E2F7/E2F8 significantly inhibited T cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (C) Impact of E2F7/E2F8 silencing on cell cycle in miR-142 KO T cells. miR-142 KO T cells were targeted for E2F7/E2F8 silencing as described in Methods, then treated with anti-CD3 and anti-CD28 Abs for 0 to 3 days. Cell-cycle analyses were processed. Combined data were from 3 independent experiments. (D) KD of E2F7/E2F8 in miR-142 KO T cells significantly improved cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (E) Essential role of E2F7/E2F8 in cell-cycle activities in WT T cells. WT T cells were targeted for E2F7/E2F8 silencing, treated with anti-CD3 and anti-CD28 Abs for 0 to 4 days, and processed for cell-cycle analyses as in C. Data were combined from 3 independent experiments. (F) KD of E2F7/E2F8 in WT T cells significantly enhanced cell proliferation as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01, Holm-Sidak method (A, C, and E); unpaired t test (B, D, and F).
Pgrna Ckb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n a pbv luc bs1 mut pmid  (Addgene inc)
90
Addgene inc n a pbv luc bs1 mut pmid
(A) Impact of overexpression of atypical E2F proteins on cell cycle in WT T cells. WT T cells were infected with E2F7/E2F8 <t>lentiviral</t> particles, treated with anti-CD3 and anti-CD28 Abs, and analyzed for cell cycle. Combined data were from 3 independent experiments. (B) Overexpression of E2F7/E2F8 significantly inhibited T cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (C) Impact of E2F7/E2F8 silencing on cell cycle in miR-142 KO T cells. miR-142 KO T cells were targeted for E2F7/E2F8 silencing as described in Methods, then treated with anti-CD3 and anti-CD28 Abs for 0 to 3 days. Cell-cycle analyses were processed. Combined data were from 3 independent experiments. (D) KD of E2F7/E2F8 in miR-142 KO T cells significantly improved cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (E) Essential role of E2F7/E2F8 in cell-cycle activities in WT T cells. WT T cells were targeted for E2F7/E2F8 silencing, treated with anti-CD3 and anti-CD28 Abs for 0 to 4 days, and processed for cell-cycle analyses as in C. Data were combined from 3 independent experiments. (F) KD of E2F7/E2F8 in WT T cells significantly enhanced cell proliferation as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01, Holm-Sidak method (A, C, and E); unpaired t test (B, D, and F).
N A Pbv Luc Bs1 Mut Pmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgrna plasmid  (Addgene inc)
90
Addgene inc pgrna plasmid
(A) Impact of overexpression of atypical E2F proteins on cell cycle in WT T cells. WT T cells were infected with E2F7/E2F8 <t>lentiviral</t> particles, treated with anti-CD3 and anti-CD28 Abs, and analyzed for cell cycle. Combined data were from 3 independent experiments. (B) Overexpression of E2F7/E2F8 significantly inhibited T cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (C) Impact of E2F7/E2F8 silencing on cell cycle in miR-142 KO T cells. miR-142 KO T cells were targeted for E2F7/E2F8 silencing as described in Methods, then treated with anti-CD3 and anti-CD28 Abs for 0 to 3 days. Cell-cycle analyses were processed. Combined data were from 3 independent experiments. (D) KD of E2F7/E2F8 in miR-142 KO T cells significantly improved cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (E) Essential role of E2F7/E2F8 in cell-cycle activities in WT T cells. WT T cells were targeted for E2F7/E2F8 silencing, treated with anti-CD3 and anti-CD28 Abs for 0 to 4 days, and processed for cell-cycle analyses as in C. Data were combined from 3 independent experiments. (F) KD of E2F7/E2F8 in WT T cells significantly enhanced cell proliferation as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01, Holm-Sidak method (A, C, and E); unpaired t test (B, D, and F).
Pgrna Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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grna expressing plasmids al08 pgrna dmd  (Addgene inc)
90
Addgene inc grna expressing plasmids al08 pgrna dmd
(A) Impact of overexpression of atypical E2F proteins on cell cycle in WT T cells. WT T cells were infected with E2F7/E2F8 <t>lentiviral</t> particles, treated with anti-CD3 and anti-CD28 Abs, and analyzed for cell cycle. Combined data were from 3 independent experiments. (B) Overexpression of E2F7/E2F8 significantly inhibited T cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (C) Impact of E2F7/E2F8 silencing on cell cycle in miR-142 KO T cells. miR-142 KO T cells were targeted for E2F7/E2F8 silencing as described in Methods, then treated with anti-CD3 and anti-CD28 Abs for 0 to 3 days. Cell-cycle analyses were processed. Combined data were from 3 independent experiments. (D) KD of E2F7/E2F8 in miR-142 KO T cells significantly improved cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (E) Essential role of E2F7/E2F8 in cell-cycle activities in WT T cells. WT T cells were targeted for E2F7/E2F8 silencing, treated with anti-CD3 and anti-CD28 Abs for 0 to 4 days, and processed for cell-cycle analyses as in C. Data were combined from 3 independent experiments. (F) KD of E2F7/E2F8 in WT T cells significantly enhanced cell proliferation as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01, Holm-Sidak method (A, C, and E); unpaired t test (B, D, and F).
Grna Expressing Plasmids Al08 Pgrna Dmd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgrna guideless  (Addgene inc)
90
Addgene inc pgrna guideless
A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome <t>targeting</t> <t>sgRNA</t> <t>(dCas9/pgRNA-non</t> target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.
Pgrna Guideless, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pre amplified  (Addgene inc)
92
Addgene inc pre amplified
A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome <t>targeting</t> <t>sgRNA</t> <t>(dCas9/pgRNA-non</t> target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.
Pre Amplified, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ay22 pgrna r5 1  (Addgene inc)
86
Addgene inc ay22 pgrna r5 1
A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome <t>targeting</t> <t>sgRNA</t> <t>(dCas9/pgRNA-non</t> target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.
Ay22 Pgrna R5 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ay23 pgrna r5 2  (Addgene inc)
86
Addgene inc ay23 pgrna r5 2
A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome <t>targeting</t> <t>sgRNA</t> <t>(dCas9/pgRNA-non</t> target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.
Ay23 Pgrna R5 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgrna non target  (Addgene inc)
90
Addgene inc pgrna non target
A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome <t>targeting</t> <t>sgRNA</t> <t>(dCas9/pgRNA-non</t> target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.
Pgrna Non Target, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Impact of overexpression of atypical E2F proteins on cell cycle in WT T cells. WT T cells were infected with E2F7/E2F8 lentiviral particles, treated with anti-CD3 and anti-CD28 Abs, and analyzed for cell cycle. Combined data were from 3 independent experiments. (B) Overexpression of E2F7/E2F8 significantly inhibited T cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (C) Impact of E2F7/E2F8 silencing on cell cycle in miR-142 KO T cells. miR-142 KO T cells were targeted for E2F7/E2F8 silencing as described in Methods, then treated with anti-CD3 and anti-CD28 Abs for 0 to 3 days. Cell-cycle analyses were processed. Combined data were from 3 independent experiments. (D) KD of E2F7/E2F8 in miR-142 KO T cells significantly improved cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (E) Essential role of E2F7/E2F8 in cell-cycle activities in WT T cells. WT T cells were targeted for E2F7/E2F8 silencing, treated with anti-CD3 and anti-CD28 Abs for 0 to 4 days, and processed for cell-cycle analyses as in C. Data were combined from 3 independent experiments. (F) KD of E2F7/E2F8 in WT T cells significantly enhanced cell proliferation as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01, Holm-Sidak method (A, C, and E); unpaired t test (B, D, and F).

Journal: The Journal of Clinical Investigation

Article Title: Mature T cell responses are controlled by microRNA-142

doi: 10.1172/JCI78753

Figure Lengend Snippet: (A) Impact of overexpression of atypical E2F proteins on cell cycle in WT T cells. WT T cells were infected with E2F7/E2F8 lentiviral particles, treated with anti-CD3 and anti-CD28 Abs, and analyzed for cell cycle. Combined data were from 3 independent experiments. (B) Overexpression of E2F7/E2F8 significantly inhibited T cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (C) Impact of E2F7/E2F8 silencing on cell cycle in miR-142 KO T cells. miR-142 KO T cells were targeted for E2F7/E2F8 silencing as described in Methods, then treated with anti-CD3 and anti-CD28 Abs for 0 to 3 days. Cell-cycle analyses were processed. Combined data were from 3 independent experiments. (D) KD of E2F7/E2F8 in miR-142 KO T cells significantly improved cell proliferation, as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. (E) Essential role of E2F7/E2F8 in cell-cycle activities in WT T cells. WT T cells were targeted for E2F7/E2F8 silencing, treated with anti-CD3 and anti-CD28 Abs for 0 to 4 days, and processed for cell-cycle analyses as in C. Data were combined from 3 independent experiments. (F) KD of E2F7/E2F8 in WT T cells significantly enhanced cell proliferation as demonstrated by 3H-TdR incorporation. Data represent combination of 3 independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01, Holm-Sidak method (A, C, and E); unpaired t test (B, D, and F).

Article Snippet: Expression plasmids were cloned by inserting PCR products into the lentiviral U6-based expression vector (Addgene, 44248) digested by BstXI and XhoI.

Techniques: Over Expression, Infection

A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome targeting sgRNA (dCas9/pgRNA-non target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.

Journal: bioRxiv

Article Title: A broad-host-range CRISPRi toolkit for silencing gene expression in Burkholderia

doi: 10.1101/618413

Figure Lengend Snippet: A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome targeting sgRNA (dCas9/pgRNA-non target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.

Article Snippet: The following plasmids have been deposited to Addgene for distribution: pAH-CTX1-rha (#129390), pAH-CTX1-rhadCas9 (#129391), pAH-CTX1-rhadCas9-native (#129392), pAH25-SceI (#129389), pSCB2-sgRNA (#129463), pgRNA-guideless (#129464), and pgRNA-non-target (#129465).

Techniques: SDS-Gel, Purification, Plasmid Preparation, Expressing

A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome targeting sgRNA (dCas9/pgRNA-non target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.

Journal: bioRxiv

Article Title: A broad-host-range CRISPRi toolkit for silencing gene expression in Burkholderia

doi: 10.1101/618413

Figure Lengend Snippet: A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome targeting sgRNA (dCas9/pgRNA-non target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.

Article Snippet: The following plasmids have been deposited to Addgene for distribution: pAH-CTX1-rha (#129390), pAH-CTX1-rhadCas9 (#129391), pAH-CTX1-rhadCas9-native (#129392), pAH25-SceI (#129389), pSCB2-sgRNA (#129463), pgRNA-guideless (#129464), and pgRNA-non-target (#129465).

Techniques: SDS-Gel, Purification, Plasmid Preparation, Expressing