Journal: International Journal of Molecular Sciences

Article Title: Proteomic Identification of Target Proteins of Thiodigalactoside in White Adipose Tissue from Diet-Induced Obese Rats

doi: 10.3390/ijms160714441

Figure Lengend Snippet: List of proteins showing differential expression between control and thiodigalactoside (TDG)-treated rats.

Article Snippet: The following antibodies were used in this study: anti-goat UCP-1, MDH2, PGM1, PPARγ, and CA3; anti-mouse CK and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA); and anti-rabbit ANXA2 (Cell Signaling Technology, Beverly, MA, USA) and PGC-1α (Santa Cruz Biotechnology).

Techniques: Quantitative Proteomics, Control, Sequencing, Transduction, Membrane

Journal: PLoS ONE

Article Title: Epigenetic Disruption of the PIWI Pathway in Human Spermatogenic Disorders

doi: 10.1371/journal.pone.0047892

Figure Lengend Snippet: Tissular expression profiling of PIWIL2 (A) and TDRD1 (B) by quantitative real-time qPCR in testis with conserved spermatogenesis (CS), maturation failure at the round spermatid (rsMF), the spermatocyte (scMF) and the spermatogonia (sgMF) stages and Sertoli cell-only syndrome (SCO). Expression levels relative to PGM1 are shown. Expression per cell profiling of PIWIL2 , displayed as expression ratio per spermatogonia/spermatocyte (x1000) (C) and expression per cell of TDRD1 , displayed as expression ratio per spermatocyte (X1000) (D) in testis with conserved spermatogenesis (CS), maturation failure at the spermatocyte (scMF), the round spermatid (rsMF), and the spermatogonia (sgMF) stages. The horizontal bar displays the mean cellular expression level. Significant differences from the control are indicated: *p<0.05; **p<0.01.

Article Snippet: Quantitative real-time PCR (qPCR) reactions were performed on an ABI 7300 real-time PCR system (Applied Biosystems) using gene-specific TaqMan Assays ( PIWIL2 : Hs00216263_m1; TDRD1 : Hs00229805_m1; PGM1 : Hs00160062_m1) and custom-designed small RNA TaqMan Assays (Applied Biosystems).

Techniques: Expressing, Control

Journal: Animal Nutrition

Article Title: Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

doi: 10.1016/j.aninu.2022.03.008

Figure Lengend Snippet: Selected differentially expressed proteins in duck liver caused by pantothenic acid deficiency (PAD).

Article Snippet: Primary antibodies (1 μg/mL) against PGM1 (A6303; ABclonal), ME1 (A3956; ABclonal), IDH1 (A13245; ABclonal), and MDH2 (A13516; ABclonal) were used.

Techniques: Binding Assay, Phospho-proteomics

Journal: Animal Nutrition

Article Title: Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

doi: 10.1016/j.aninu.2022.03.008

Figure Lengend Snippet: Integrated pathway analysis of liver proteins and metabolites in ducks significantly affected by pantothenic acid deficiency (PAD).

Article Snippet: Primary antibodies (1 μg/mL) against PGM1 (A6303; ABclonal), ME1 (A3956; ABclonal), IDH1 (A13245; ABclonal), and MDH2 (A13516; ABclonal) were used.

Techniques: Phospho-proteomics

Journal: Animal Nutrition

Article Title: Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

doi: 10.1016/j.aninu.2022.03.008

Figure Lengend Snippet: Western blot analysis of phosphoglucomutase 1 (PGM1; A), malic enzyme 1 (ME1; B), isocitrate dehydrogenase (IDH1; C), and malate dehydrogenase 2 (MDH2; D) protein expression of liver tissue of ducks in the pantothenic acid deficient (PAD) and Control (CON) groups. Loading control, vinculin, was used to normalize the levels of PGM1, ME1, IDH1, and MDH2. Representative Western blots are shown. Values are means with their standard errors. a, b Mean values with unlike letters were significantly different ( P < 0.05). Data were analyzed by the Student's t -test.

Article Snippet: Primary antibodies (1 μg/mL) against PGM1 (A6303; ABclonal), ME1 (A3956; ABclonal), IDH1 (A13245; ABclonal), and MDH2 (A13516; ABclonal) were used.

Techniques: Western Blot, Expressing, Control

Journal: Animal Nutrition

Article Title: Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

doi: 10.1016/j.aninu.2022.03.008

Figure Lengend Snippet: Disturbed metabolic pathways in liver tissue caused by pantothenic acid deficiency (PAD) based on proteomics and metabolomics profiles. Regulation is color and arrow coded in which red arrow ( Image 1 ) stands for upregulated, blue arrow ( Image 2 ) for downregulated in PAD group compared to control group. PYGL = alpha-1,4 glucan phosphorylase; PGM1 = phosphoglucomutase 1; FADH2 = Flavin adenine dinucleotide; TCA = tricarboxylic acid.

Article Snippet: Primary antibodies (1 μg/mL) against PGM1 (A6303; ABclonal), ME1 (A3956; ABclonal), IDH1 (A13245; ABclonal), and MDH2 (A13516; ABclonal) were used.

Techniques: Control

Journal: Acta Pharmaceutica Sinica. B

Article Title: Metabolic engineering of yeasts for green and sustainable production of bioactive ginsenosides F2 and 3 β ,20 S -Di- O -Glc-DM

doi: 10.1016/j.apsb.2022.04.012

Figure Lengend Snippet: Strains constructed in this study.

Article Snippet: The genes ( INO2 , PGM1 , PGM2 , UGP1 ), the promoters ( ENO2 , TEF2 ) and the terminators ( FBA1 , IDP1 ) from S. cerevisiae were all synthesized by GenScript (Nanjing, China).

Techniques: Construct, Marker