Journal: Metabolites

Article Title: Rutin Maintains the Thermogenic Phenotype of Beige Adipocytes and Concomitantly Suppresses Mitophagy Against Obesity in HFD Mice

doi: 10.3390/metabo16010012

Figure Lengend Snippet: Rutin preserves the molecular characteristics of beige adipocytes following withdrawal from cold exposure. ( A , B ) Western blot analysis and interpretation of brown adipocyte proteins UCP1, PGC-1α and PRDM16 in ND, HFD, and HFD + Rutin groups under cold exposure and withdrawal conditions. n = 3. ( C ) UCP1 immunohistochemistry in ND, HFD, and HFD + Rutin groups during cold exposure and withdrawal. Scale bar is 100 μm. Data represent the means ± SEMs. Significance was determined by two-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. Statistical significance is represented as follows: ns indicates no significant difference; * p < 0.05, ** p < 0.01 *** p < 0.001 vs. Cold group; ## p < 0.01, ### p < 0.001 vs. HFD + −Cold group; $ p < 0.05, $$ p < 0.01, $$$ p < 0.001 vs. HFD + Cold group.

Article Snippet: The following antibodies were obtained from the named sources: PRDM16 (Affinit Biosciences, Hefei, China; DF13303, 1:1000), PGC-1α (Abways, Shanghai, China; CY6630, 1:1000), UCP1 (Abclonal, Woburn, MA, USA; A5857, 1:1000), β-actin (Abclonal, Woburn, MA, USA; AC038, 1:2000), PINK1 (Abclonal, Woburn, MA, USA; A7131, 1:1000), and Parkin (BOSTER, Pleasanton, CA, USA; BM4909, 1:1000).

Techniques: Western Blot, Immunohistochemistry