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Promega
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BioResource International Inc
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Lonza
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Becton Dickinson
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Verlag GmbH
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BioLeaders Corp
endotoxin-free g-pgas with different average molecular weights (1, 5, 50, 500, 2000, and 5000 kda) ![]() Endotoxin Free G Pgas With Different Average Molecular Weights (1, 5, 50, 500, 2000, And 5000 Kda), supplied by BioLeaders Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/pgas/pm24016850-45-0-24?v=BioLeaders+Corp Average 90 stars, based on 1 article reviews
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Becton Dickinson
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BioNano Genomics
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NCIMB Ltd
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BioLeaders Corp
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Shifang Zhixin Chemical Co Ltd
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Image Search Results
Journal: Cellular signalling
Article Title: E3 ubiquitin ligase NKLAM ubiquitinates STAT1 and positively regulates STAT1-mediated transcriptional activity
doi: 10.1016/j.cellsig.2016.08.014
Figure Lengend Snippet: NKLAM positively affects STAT1-mediated transcriptional activity. A) WT (black column) and NKLAM-KO (white column) BMDM were nucleofected with pGAS-luc plasmid then stimulated with 100 U/mL IFNγ for 6 h and assayed for luciferase activity; *p < 0.05; n = 5. B–C) WT (black column) and NKLAM-KO (white column) BMDM were treated with 100 U/mL IFNγ for 3 or 6 h. The expression of iNOS and CCL5 was determined by quantitative RT-PCR. The fold change in mRNA levels (mean ± SEM) is expressed relative to untreated cultures (solid line set at 1). Graph represents at least 3 independent experiments. *p < 0.05; n = 3.
Article Snippet: WT or NKLAM-KO macrophages (4 × 10 6 ) were mixed with 3 μg of the
Techniques: Activity Assay, Plasmid Preparation, Luciferase, Expressing, Quantitative RT-PCR