Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Spike raster plots illustrating the pattern of activity of a representative well of the iCell Glutaneuron – iCell Astrocytes co-culture before exposure (left) and the same well following exposure (right) to PFOS 10 µM ( A ) or PFOA 100 µM ( B ). Each row depicts one electrode in a well and each tick mark represents one spike in a 50 s interval. Spikes are depicted in black and bursts are depicted in blue.

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Activity Assay, Co-Culture Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Modulation of spontaneous neuronal activity of rat primary cortical neurons exposed to PFOS (black) or PFOA (grey) following acute exposure. Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 18–20 wells and N = 4 plates. *Indicates p

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Activity Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Modulation of spontaneous neuronal activity of iCell Glutaneuron – iCell Astrocytes co-culture exposed to PFOS (black) or PFOA (grey) following acute exposure. Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 3–17 wells and N = 3–5 plates. *Indicates p

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Activity Assay, Co-Culture Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Modulation of spontaneous neuronal activity of SynFire iNS co-culture with PFOS (black) or PFOA (grey). Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 7–9 wells and N = 2 plates. *Indicates p

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Activity Assay, Co-Culture Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Antagonistic effects of PFOS and PFOA on the human GABA A receptor. Example recordings of inhibition of GABA-evoked ion currents by co-application of PFOS (1 µM, a , c ) or PFOA (10 µM, b , d ) with GABA (at low and high effective GABA concentration, respectively). PFOS-induced inhibition is poorly reversible ( a , c ), whereas PFOA-induced inhibition is reversed within seconds ( b , d ). Scale bar applies to all traces. Concentration-response curves show the concentration-dependent inhibition of GABA-evoked responses by PFOS ( e ) and PFOA ( f ) on the human GAB A receptor with at low ( e , f ; solid line) and high ( e,f ; dashed line) effective GABA concentrations. Inhibition is presented as percentage of the GABA-evoked response (mean ± SEM, n = 3–4 oocytes per concentration from N = 1–2 batches).

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Inhibition, Concentration Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Heatmap of the effects of PFOS (light green) and PFOA (darker green) on selected metric parameters on rat primary cortical neurons (top; grey), iCell Glutaneuron – iCell Astrocytes (middle; black) and SynFire iNS co-culture (bottom; green). Colour scaling is based on the magnitude change in percentages relative to vehicle control based on n = 18–20 wells and N = 4 plates for rat primary cortical neurons; n = 3–17 wells and N = 3–5 plates for iCell Glutaneuron – iCell Astrocytes; n = 6–9 wells N = 2 plates for SynFire iNS co-culture.

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Co-Culture Assay

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: Predicted activation of upstream regulators in response to PFOA or PFOS

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques: Activation Assay

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: In silico docking studies of PFOA and PFOS to HNF4α

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques: In Silico

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: Genes upregulated by PFOA or PFOS and NANOG expression

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques: Expressing

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: HNF4α regulated genes altered in response to PFOA and PFOS

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques:

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: PFOA- and PFOS-induced cytotoxicity

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques:

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: Human serum concentrations of PFOA and PFOS within different subpopulations of exposure in the United States

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques:

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: Genes downregulated by PFOA or PFOS

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques:

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: PFOA and PFOS ligands docked to HNF4α

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques:

Journal: Toxicology and applied pharmacology

Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction

doi: 10.1016/j.taap.2016.05.001

Figure Lengend Snippet: Concentration-response of HNF4α protein expression in hepatocytes exposed to PFOA or PFOS

Article Snippet: As expected, residents of the areas surrounding cities including Decatur, Alabama and Cottage Grove, Minnesota, where the 3M company manufactured PFOA and PFOS, had higher concentrations in their blood, as a consequence of watershed contamination.

Techniques: Concentration Assay, Expressing