Journal: PPAR Research

Article Title: Gene Expression Profiling in Wild-Type and PPARα-Null Mice Exposed to Perfluorooctane Sulfonate Reveals PPARα-Independent Effects

doi: 10.1155/2010/794739

Figure Lengend Snippet: Expression of a group of well characterized markers of PPAR α transactivation in WT and Null mice. The response to PFOS in WT mice was less robust than that previously observed for either PFOA or Wy14,643. Red or green correspond to average up- or down- regulation, respectively.

Article Snippet: PCR dose groups consisted of 4 animals per group and were treated for seven-days with either 10 mg/kg/day PFOS, 3 mg/kg/day PFOA (ammonium salt, catalog no. 77262, Sigma-Aldrich) in 0.5% Tween 20, or 50 mg/kg/day Wy-14,643 (catalog no. C7081, Sigma-Aldrich) in 0.5% methylcellulose, along with vehicle controls.

Techniques: Expressing, Mouse Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Spike raster plots illustrating the pattern of activity of a representative well of the iCell Glutaneuron – iCell Astrocytes co-culture before exposure (left) and the same well following exposure (right) to PFOS 10 µM ( A ) or PFOA 100 µM ( B ). Each row depicts one electrode in a well and each tick mark represents one spike in a 50 s interval. Spikes are depicted in black and bursts are depicted in blue.

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Activity Assay, Co-Culture Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Modulation of spontaneous neuronal activity of rat primary cortical neurons exposed to PFOS (black) or PFOA (grey) following acute exposure. Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 18–20 wells and N = 4 plates. *Indicates p

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Activity Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Modulation of spontaneous neuronal activity of iCell Glutaneuron – iCell Astrocytes co-culture exposed to PFOS (black) or PFOA (grey) following acute exposure. Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 3–17 wells and N = 3–5 plates. *Indicates p

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Activity Assay, Co-Culture Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Modulation of spontaneous neuronal activity of SynFire iNS co-culture with PFOS (black) or PFOA (grey). Effects on mean spike rate (MSR; A ), mean burst rate (MBR; B ), mean network burst rate (MNBR; C ), burst duration ( D ) and network burst duration ( E ) are depicted as average in % change of control (solvent control set to 100%; dashed line) ± SEM from n = 7–9 wells and N = 2 plates. *Indicates p

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Activity Assay, Co-Culture Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Antagonistic effects of PFOS and PFOA on the human GABA A receptor. Example recordings of inhibition of GABA-evoked ion currents by co-application of PFOS (1 µM, a , c ) or PFOA (10 µM, b , d ) with GABA (at low and high effective GABA concentration, respectively). PFOS-induced inhibition is poorly reversible ( a , c ), whereas PFOA-induced inhibition is reversed within seconds ( b , d ). Scale bar applies to all traces. Concentration-response curves show the concentration-dependent inhibition of GABA-evoked responses by PFOS ( e ) and PFOA ( f ) on the human GAB A receptor with at low ( e , f ; solid line) and high ( e,f ; dashed line) effective GABA concentrations. Inhibition is presented as percentage of the GABA-evoked response (mean ± SEM, n = 3–4 oocytes per concentration from N = 1–2 batches).

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Inhibition, Concentration Assay

Journal: Scientific Reports

Article Title: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro

doi: 10.1038/s41598-020-62152-2

Figure Lengend Snippet: Heatmap of the effects of PFOS (light green) and PFOA (darker green) on selected metric parameters on rat primary cortical neurons (top; grey), iCell Glutaneuron – iCell Astrocytes (middle; black) and SynFire iNS co-culture (bottom; green). Colour scaling is based on the magnitude change in percentages relative to vehicle control based on n = 18–20 wells and N = 4 plates for rat primary cortical neurons; n = 3–17 wells and N = 3–5 plates for iCell Glutaneuron – iCell Astrocytes; n = 6–9 wells N = 2 plates for SynFire iNS co-culture.

Article Snippet: Stock solutions of PFOS ( > 98% purity, Fluka, Zwijndrecht, The Netherlands) and PFOA ( > 96% purity, Acros Organics, Geel, Belgium) were made in purity-checked dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Co-Culture Assay

Journal: Environmental Health Perspectives

Article Title: Correlations between Prenatal Exposure to Perfluorinated Chemicals and Reduced Fetal Growth

doi: 10.1289/ehp.11681

Figure Lengend Snippet: Box and whisker plots of PFOS and PFOA concentrations in maternal serum. Horizontal lines inside boxes indicate the median, boxes represent the interquartile range, whiskers indicate the most extreme data points

Article Snippet: We obtained PFOS [molecular weight (MW), 538.23 Da; 98% purity] and PFOA (MW, 414.07 Da; > 90% purity) from Fluka Chemie AG (Buchs, Switzerland).

Techniques: Whisker Assay

Journal: Environmental Health Perspectives

Article Title: Half-Life of Serum Elimination of Perfluorooctanesulfonate,Perfluorohexanesulfonate, and Perfluorooctanoate in Retired Fluorochemical Production Workers

doi: 10.1289/ehp.10009

Figure Lengend Snippet: Semi-log plots of PFOS, PFHS, and PFOA concentrations by time (days) for subjects 1–26. Data points indicate observed values and the line shows predicted value.

Article Snippet: Chemicals Potassium salt of PFOS, potassium salt of PFHS, and ammonium salt of PFOA were obtained from internal sources at the 3M Company (St. Paul, MN).

Techniques:

Journal: Environmental Health Perspectives

Article Title: Half-Life of Serum Elimination of Perfluorooctanesulfonate,Perfluorohexanesulfonate, and Perfluorooctanoate in Retired Fluorochemical Production Workers

doi: 10.1289/ehp.10009

Figure Lengend Snippet: Half-life (years) of serum elimination for PFOS, PFHS, and PFOA (in ascending order for PFOS) for 26 retired fluorochemical production workers by subject number.

Article Snippet: Chemicals Potassium salt of PFOS, potassium salt of PFHS, and ammonium salt of PFOA were obtained from internal sources at the 3M Company (St. Paul, MN).

Techniques: