Journal: Discover oncology

Article Title: Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma.

doi: 10.1007/s12672-025-01859-y

Figure Lengend Snippet: Fig. 1 SUMF2 mRNA expres- sion was associated with poor survival in patients with blad- der UC (BLCA). A OncoPrint showed SUMF1 and SUMF2 amplification in 4% and 2.4% of UC tumors, respectively. Colors indicate the type of genetic change (missense, in-frame, truncated, amplifica- tion, deletion, or fusion), and the different cohorts are listed below the OncoPrint. B SUMF1 and SUMF2 mRNA levels in multiple cancer types. Kaplan–Meier curve for overall survival (C) and disease-free survival (D), according to the mRNA expression of SUMF1 and SUMF2 in patients with BLCA, using TCGA datasets (GEPIA). HR hazard ratio

Article Snippet: Next, the slides were incubated with the SUMF2 primary antibody (1:100;11210-1-AP; Proteintech).

Techniques: Amplification, Expressing

Journal: Discover oncology

Article Title: Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma.

doi: 10.1007/s12672-025-01859-y

Figure Lengend Snippet: Fig. 2 The expression of the SUMF2 protein was elevated in the tissues of bladder cancer. A SUMF2 expression in BLCA specimens was quantified, and the Student’s t-test was used to evaluate significance. *P < 0.05. B Representative images of SUMF2 expression in BLCA tis- sues. C SUMF2 expression in different Tumor status of BLCA tissues

Article Snippet: Next, the slides were incubated with the SUMF2 primary antibody (1:100;11210-1-AP; Proteintech).

Techniques: Expressing

Journal: Discover oncology

Article Title: Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma.

doi: 10.1007/s12672-025-01859-y

Figure Lengend Snippet: Fig. 3 SUMF2 expression was upregulated in UTUC. A Quantification of SUMF2 expression in 74 paired UTUC specimens and significance, determined using the Student’s t-test. **P < 0.01. B Representative images of SUMF2 expression in upper tract UC (UTUC) tissues. C Kaplan– Meier survival curves for overall survival and disease-free survival in patients with UTUC according to SUMF2 protein expression

Article Snippet: Next, the slides were incubated with the SUMF2 primary antibody (1:100;11210-1-AP; Proteintech).

Techniques: Expressing

Journal: Discover oncology

Article Title: Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma.

doi: 10.1007/s12672-025-01859-y

Figure Lengend Snippet: Fig. 4 SUMF2 knockdown inhibits migration and invasion in UC cells. A RT-qPCR analysis of SUMF2 expression in UC cancer cell lines. B RT- qPCR examination of SUMF2 expression following infection with shRNA. C The impact of SUMF2 knockdown on 5637 cell growth. D Down- regulation of SUMF2 inhibited migration and invasion viabilities of 5637 cells. Data are presented as mean ± SD. **P < 0.01; *P < 0.05

Article Snippet: Next, the slides were incubated with the SUMF2 primary antibody (1:100;11210-1-AP; Proteintech).

Techniques: Knockdown, Migration, Quantitative RT-PCR, Expressing, Infection, shRNA

Journal: Discover oncology

Article Title: Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma.

doi: 10.1007/s12672-025-01859-y

Figure Lengend Snippet: Fig. 5 Correlation analysis between SUMF2 and SULF family expression in patients with BLCA. A Correlation of SUMF2 with SULF1 and SULF2 mRNA expression using TCGA datasets of BLCA (ENCORI). B Kaplan–Meier analysis of overall survival, according to SULF1 and SULF2 mRNA expression in BLCA. C Kaplan–Meier survival curves for SUMF2 and SULF1 and the SUMF2/SULF2 axis in patients with BLCA (using TCGA data)

Article Snippet: Next, the slides were incubated with the SUMF2 primary antibody (1:100;11210-1-AP; Proteintech).

Techniques: Expressing

Journal: Discover oncology

Article Title: Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma.

doi: 10.1007/s12672-025-01859-y

Figure Lengend Snippet: Fig. 6 The expression of high SUMF2/ low FBXW7 axis is correlated with poor survival among patients with BLCA. A Putative binding and interaction targets of SUMF2 were identified from Pathway Commons. B Analysis of the correlation between SUMF2 and FBXW7 mRNA expression using ENCORI. C Analysis of overall survival according to FBXW7 mRNA expression (TCGA). D Kaplan–Meier analysis of overall survival among patients with BLCA by examining the combination of SUMF2 and FBXW7 mRNA expression levels. other: SUMF2 low/FBXW7 low and SUMF2 high/FBXW7 high mRNA levels

Article Snippet: Next, the slides were incubated with the SUMF2 primary antibody (1:100;11210-1-AP; Proteintech).

Techniques: Expressing, Binding Assay

Journal: Applied and Environmental Microbiology

Article Title: Rapid and Simple Universal Escherichia coli Genotyping Method Based on Multiple-Locus Variable-Number Tandem-Repeat Analysis Using Single-Tube Multiplex PCR and Standard Gel Electrophoresis

doi: 10.1128/AEM.02812-18

Figure Lengend Snippet: Dendrogram of the MLVA (A) and PFGE (B) results for E. coli O157:H7 strains. The dendrograms were constructed by BioNumerics software using UPGMA (A and B) and the Dice algorithm (A) based on the band profile on the electrophoresis gel. The PFGE profile was initially reported by aNRC-EC at the Robert Debré Hospital. The PFGE types C, C′, S, Sʺ, and Sʺʹ are profiles differentiated by less than three bands and considered similar according to the criteria of Tenover et al. (20). The dotted lines correspond to the 95% similarity cutoff.

Article Snippet: All the 21 O157:H7 E. coli isolates were also typed by the PFGE method by the Associated National Reference Center for E. coli (aNRC-EC) for epidemiological purposes.

Techniques: Construct, Software, Electrophoresis

Journal:

Article Title: First Characterization of Fluoroquinolone Resistance in Streptococcus suis ▿

doi: 10.1128/AAC.00972-06

Figure Lengend Snippet: PFGE fingerprint patterns of S. suis isolates used in the present study using the ApaI endonuclease. Lanes 1 to 6, fluoroquinolone-susceptible strains; lanes 7 to 17, fluoroquinolone-resistant isolates. The first and last lanes are bacteriophage lambda ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017. Analysis was performed with QuantityOne software (Bio-Rad, Richmond, CA).

Article Snippet: The first and last lanes are bacteriophage lambda ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017.

Techniques: Software