pf 475 Search Results


pf-475 (3 or 30 mg·kg −1 )  (Dawley Inc)
90
Dawley Inc pf-475 (3 or 30 mg·kg −1 )
a. Representative immunoblots for detecting Lrrk2 and Rab proteins in forebrain lysates from Charles Rivers(Sprague Dawley, (CD(SD)) rats treated with <t>PF-475</t> (3 or 30 mg·kg−1) and vehicle (Veh) controls. Tissue was collected two hours post-dosing. Quantification of b. total Lrrk2 protein levels normalized to HSC70, and c. pS935-Lrrk2 levels normalized to total Lrrk2 protein. Quantification of pT73-Rab10 levels normalized to d. HSC70 and e. total Rab10 protein. Quantification of pS106-Rab12 levels normalized to f. HSC70 and g. total Rab12. Data are graphed as average measures from triplicate western blots for biological replicates (N=3 rats per group). All values are presented as fold changes to the vehicle treated group. Bar graphs show group means ± SEM. Significance between groups was determined by one-way ANOVA with Dunnett’s multiple comparison test with respect to the indicated group and the vehicle treated group mean. *p< 0.05, **p< 0.01. All other comparisons not indicated by asterisks were not significant.
Pf 475 (3 Or 30 Mg·Kg −1 ), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pf-475 (3 or 30 mg·kg −1 )/product/Dawley Inc
Average 90 stars, based on 1 article reviews
pf-475 (3 or 30 mg·kg −1 ) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
pf-3,475,952  (Pfizer Inc)
90
Pfizer Inc pf-3,475,952
a. Representative immunoblots for detecting Lrrk2 and Rab proteins in forebrain lysates from Charles Rivers(Sprague Dawley, (CD(SD)) rats treated with <t>PF-475</t> (3 or 30 mg·kg−1) and vehicle (Veh) controls. Tissue was collected two hours post-dosing. Quantification of b. total Lrrk2 protein levels normalized to HSC70, and c. pS935-Lrrk2 levels normalized to total Lrrk2 protein. Quantification of pT73-Rab10 levels normalized to d. HSC70 and e. total Rab10 protein. Quantification of pS106-Rab12 levels normalized to f. HSC70 and g. total Rab12. Data are graphed as average measures from triplicate western blots for biological replicates (N=3 rats per group). All values are presented as fold changes to the vehicle treated group. Bar graphs show group means ± SEM. Significance between groups was determined by one-way ANOVA with Dunnett’s multiple comparison test with respect to the indicated group and the vehicle treated group mean. *p< 0.05, **p< 0.01. All other comparisons not indicated by asterisks were not significant.
Pf 3,475,952, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pf-3,475,952/product/Pfizer Inc
Average 90 stars, based on 1 article reviews
pf-3,475,952 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


a. Representative immunoblots for detecting Lrrk2 and Rab proteins in forebrain lysates from Charles Rivers(Sprague Dawley, (CD(SD)) rats treated with PF-475 (3 or 30 mg·kg−1) and vehicle (Veh) controls. Tissue was collected two hours post-dosing. Quantification of b. total Lrrk2 protein levels normalized to HSC70, and c. pS935-Lrrk2 levels normalized to total Lrrk2 protein. Quantification of pT73-Rab10 levels normalized to d. HSC70 and e. total Rab10 protein. Quantification of pS106-Rab12 levels normalized to f. HSC70 and g. total Rab12. Data are graphed as average measures from triplicate western blots for biological replicates (N=3 rats per group). All values are presented as fold changes to the vehicle treated group. Bar graphs show group means ± SEM. Significance between groups was determined by one-way ANOVA with Dunnett’s multiple comparison test with respect to the indicated group and the vehicle treated group mean. *p< 0.05, **p< 0.01. All other comparisons not indicated by asterisks were not significant.

Journal: Brain research

Article Title: Genetic background influences LRRK2-mediated Rab phosphorylation in the rat brain

doi: 10.1016/j.brainres.2021.147372

Figure Lengend Snippet: a. Representative immunoblots for detecting Lrrk2 and Rab proteins in forebrain lysates from Charles Rivers(Sprague Dawley, (CD(SD)) rats treated with PF-475 (3 or 30 mg·kg−1) and vehicle (Veh) controls. Tissue was collected two hours post-dosing. Quantification of b. total Lrrk2 protein levels normalized to HSC70, and c. pS935-Lrrk2 levels normalized to total Lrrk2 protein. Quantification of pT73-Rab10 levels normalized to d. HSC70 and e. total Rab10 protein. Quantification of pS106-Rab12 levels normalized to f. HSC70 and g. total Rab12. Data are graphed as average measures from triplicate western blots for biological replicates (N=3 rats per group). All values are presented as fold changes to the vehicle treated group. Bar graphs show group means ± SEM. Significance between groups was determined by one-way ANOVA with Dunnett’s multiple comparison test with respect to the indicated group and the vehicle treated group mean. *p< 0.05, **p< 0.01. All other comparisons not indicated by asterisks were not significant.

Article Snippet: Representative immunoblots for detecting Lrrk2 and Rab proteins in forebrain lysates from Charles Rivers(Sprague Dawley, (CD(SD)) rats treated with PF-475 (3 or 30 mg·kg −1 ) and vehicle (Veh) controls.

Techniques: Western Blot

a. Representative immunoblots for detecting Lrrk2 and Rab proteins in forebrain lysates from Lrrk2 knockout (KO) and Long Evans (LE) rats treated with a high dose of PF-475 (30 mg·kg−1) or vehicle control. Tissue was collected two hours post-dosing. Quantification of b. total Lrrk2 protein levels normalized to HSC70 and c. pS935-Lrrk2 levels normalized to total Lrrk2 protein. Quantification of pT73-Rab10 levels normalized to d. HSC70 and e. total Rab10 protein. Quantification of pS106-Rab12 levels normalized to f. HSC70 and g. total Rab12. Data are graphed as average measures from triplicate western blots for biological replicates (N=3 rats per group). All values are presented as fold changes to the vehicle treated group. Bar graphs show group means ± SEM. Significance between treated and untreated (vehicle) controls was determined using two-tailed unpaired t-tests. Data in panels d and e were log transformed prior to t-tests to better approximate normal group distributions. *p< 0.05, **p< 0.01, ***p< 0.001, ns is not significant.

Journal: Brain research

Article Title: Genetic background influences LRRK2-mediated Rab phosphorylation in the rat brain

doi: 10.1016/j.brainres.2021.147372

Figure Lengend Snippet: a. Representative immunoblots for detecting Lrrk2 and Rab proteins in forebrain lysates from Lrrk2 knockout (KO) and Long Evans (LE) rats treated with a high dose of PF-475 (30 mg·kg−1) or vehicle control. Tissue was collected two hours post-dosing. Quantification of b. total Lrrk2 protein levels normalized to HSC70 and c. pS935-Lrrk2 levels normalized to total Lrrk2 protein. Quantification of pT73-Rab10 levels normalized to d. HSC70 and e. total Rab10 protein. Quantification of pS106-Rab12 levels normalized to f. HSC70 and g. total Rab12. Data are graphed as average measures from triplicate western blots for biological replicates (N=3 rats per group). All values are presented as fold changes to the vehicle treated group. Bar graphs show group means ± SEM. Significance between treated and untreated (vehicle) controls was determined using two-tailed unpaired t-tests. Data in panels d and e were log transformed prior to t-tests to better approximate normal group distributions. *p< 0.05, **p< 0.01, ***p< 0.001, ns is not significant.

Article Snippet: Representative immunoblots for detecting Lrrk2 and Rab proteins in forebrain lysates from Charles Rivers(Sprague Dawley, (CD(SD)) rats treated with PF-475 (3 or 30 mg·kg −1 ) and vehicle (Veh) controls.

Techniques: Western Blot, Knock-Out, Two Tailed Test, Transformation Assay