Journal: Analytical and bioanalytical chemistry

Article Title: Optical approaches for single cell and subcellular analysis of GPCR-G protein signaling

doi: 10.1007/s00216-019-01774-6

Figure Lengend Snippet: FRET and BRET based biosensors

Article Snippet: Therefore, appropriate FRET or BRET probes can be designed to examine intermolecular receptor-G proteins and G protein-G protein interactions, thus measuring GPCR and G protein activation in real-time [ 126 , 127 , 122 ]. table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Biosensor Name Target Number of molecules Physical type Pubmed ID Addgene plasmid # Gαi3 v1 Gαi Bimolecular or other FRET 16371464 Gαi-Gβ1 Gαi Bimolecular or other FRET 14673086 Gαi-Gγ2 Gαi Unimolecular FRET 14673086 Gαi2 Sensor v2 Gαi Bimolecular or other FRET 26799488 #69624 Gαi1 Sensor v2 Gαi Bimolecular or other FRET 26799488 #69623 RLucll-117-Gαs + GFP10-GY1 Gαs Bimolecular or other BRET 27499021 Gαs Sensor Gαs Bimolecular or other FRET 16963443 Gαq Sensor (v2) Gαq Bimolecular or other FRET 21619590 Gα13 Sensor (v2) Gα13 Unimolecular FRET 29505611 #112933 HCN2-camps cAMP Unimolecular FRET 17038640 Epac1-camps cAMP Unimolecular FRET 15231839 Epac2-camps cAMP Unimolecular FRET 15231839 mICNBD-FRET cAMP Unimolecular FRET 27003291 CAMYEL cAMP Unimolecular BRET 17283075 BFP-PMCA-GFP Ca 2+ Unimolecular FRET 17901055 AKAR4 PKA Unimolecular FRET #61619 CKAR PKC Unimolecular FRET 12782683 #14860 Open in a separate window FRET and BRET based biosensors 5.1.

Techniques: Plasmid Preparation