Journal: International Journal of Molecular Sciences

Article Title: Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

doi: 10.3390/ijms241914418

Figure Lengend Snippet: Multiple sequence alignments of 14-3-3 ( A ) and GRA10 ( B ) from different parasites. ( A ) Multiple alignments of E. intestinalis 14-3-3 with 14-3-3 proteins from other parasites: Eimeria magna (GenBank: OQ632930), Eimeria stiedaei (GenBank: UDM59931.1), Eimeria tenella (GenBank: XP_013229533.1), Cyclospora cayetanensis (GenBank: XP_026194077.1), and Toxoplasma gondii (GenBank: XP_002365409.1). ( B ) Multiple alignments of E. intestinalis GRA10 with GRA10 proteins from other parasites: Cyclospora cayetanensis (GenBank: OEH80410.1), Toxoplasma gondii (GenBank: KFG61447.1), Besnoitia besnoiti (GenBank: XP_029219573.1), Eimeria maxima (GenBank: XP_013333610.1), and Eimeria acervulina (GenBank: XP_013251490.1). Blue shading indicates conserved amino acid residues. Red boxes indicate B-cell epitopes.

Article Snippet: The recombinant pet-32a (+)- Ei -14-3-3 and pET-32a (+)- Ei- GRA10 plasmids were transformed into E. coli BL21 (DE3) (Tiangen).

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

doi: 10.3390/ijms241914418

Figure Lengend Snippet: Expression of r Ei- 14-3-3 and r Ei- GRA10 in E. coli . M: protein markers; lanes 1–3: r Ei- 14-3-3; lane 4: r Ei- GRA10. Red arrows are used to indicate bands.

Article Snippet: The recombinant pet-32a (+)- Ei -14-3-3 and pET-32a (+)- Ei- GRA10 plasmids were transformed into E. coli BL21 (DE3) (Tiangen).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

doi: 10.3390/ijms241914418

Figure Lengend Snippet: Western blot results. M: protein marker lane 1: r Ei- 14-3-3 incubated with positive sera; lane 3: r Ei -GRA10 incubated with positive sera; lane 2: r Ei- 14-3-3 incubated with negative sera; lane 4: r Ei -GRA10 incubated with negative sera. Black arrows are used to indicate bands.

Article Snippet: The recombinant pet-32a (+)- Ei -14-3-3 and pET-32a (+)- Ei- GRA10 plasmids were transformed into E. coli BL21 (DE3) (Tiangen).

Techniques: Western Blot, Marker, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

doi: 10.3390/ijms241914418

Figure Lengend Snippet: Lesions in the intestine of each group after E. intestinalis challenge. ( A ) PBS-infected group: intestinal tract bleeding; ( B ) Trx-His-S-infected group: intestinal wall thickening; ( C ) uninfected group; ( D ) r Ei -14-3-3 immunized group: slight intestinal wall thickening; ( E ) r Ei -GRA10 immunized group: slight intestinal wall thickening. Black arrow: intestinal wall bleeding or thickening.

Article Snippet: The recombinant pet-32a (+)- Ei -14-3-3 and pET-32a (+)- Ei- GRA10 plasmids were transformed into E. coli BL21 (DE3) (Tiangen).

Techniques: Infection

Journal: International Journal of Molecular Sciences

Article Title: Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

doi: 10.3390/ijms241914418

Figure Lengend Snippet: Statistical results of each data item.

Article Snippet: The recombinant pet-32a (+)- Ei -14-3-3 and pET-32a (+)- Ei- GRA10 plasmids were transformed into E. coli BL21 (DE3) (Tiangen).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

doi: 10.3390/ijms241914418

Figure Lengend Snippet: Changes in anti-r Ei -14-3-3 ( A ) and anti-r Ei -GRA10 ( B ) IgG levels in the sera of each group.

Article Snippet: The recombinant pet-32a (+)- Ei -14-3-3 and pET-32a (+)- Ei- GRA10 plasmids were transformed into E. coli BL21 (DE3) (Tiangen).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

doi: 10.3390/ijms241914418

Figure Lengend Snippet: Primers for Ei - 14-3-3 and Ei - GRA10.

Article Snippet: The recombinant pet-32a (+)- Ei -14-3-3 and pET-32a (+)- Ei- GRA10 plasmids were transformed into E. coli BL21 (DE3) (Tiangen).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

doi: 10.3390/ijms241914418

Figure Lengend Snippet: Grouping and dosing of the experimental rabbits.

Article Snippet: The recombinant pet-32a (+)- Ei -14-3-3 and pET-32a (+)- Ei- GRA10 plasmids were transformed into E. coli BL21 (DE3) (Tiangen).

Techniques: Sterility