peroxidase-affinipure Search Results


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  • 96
    Jackson Immuno peroxidase affinipure
    Peroxidase Affinipure, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Jackson Immuno peroxidase affinipure donkey anti rabbit igg
    Peroxidase Affinipure Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Jackson Immuno peroxidase affinipure donkey anti goat igg
    Peroxidase Affinipure Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Jackson Immuno peroxidase affinipure donkey anti mouse igg
    Peroxidase Affinipure Donkey Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Jackson Immuno peroxidase affinipure rabbit anti mouse igg
    Peroxidase Affinipure Rabbit Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Jackson Immuno peroxidase affinipure goat anti rat igg
    Dose-response curves of Ca 2+ uptake in A549 cells and Western blot panel showing CHRNA7 gene silencing. Cells were treated with various concentrations of APS8 ( A ; 10, 5, 2.5, 1, 0.75, 0.5, 0.25, 0.1, 0.05, 0.01 µM) or ACh ( B ; 100, 40, 20, 10, 5, 2.5, 1, 0.5, 0.1, 0.02 µM) or preincubated (20 min, RT) with various concentrations of APS8 and then treated with 100 µM ACh ( C ). The A549 cells were silenced with CHRNA7-targeted or nontargeting (negative control) siRNAs. Data are relative fluorescence units (RFU), as maximum response minus minimum response, divided by the minimum response. The curves and their 95% confidence intervals were obtained by fitting the data with nonlinear regression function (GraphPad Prism). Recombinant human α7 nAChR (#H00001139-P01, Abnova; Lane 1) and lysates of A549 cells treated with nontargeting siRNA (Lane 2) or siRNA targeting CHRNA7 (Lanes 3 and 4) were separated by SDS-PAGE followed by Western blotting ( D ) using rabbit antibodies against human α7 nAChR (1:500; #HPA029422, Sigma-Aldrich) and Peroxidase <t>AffiniPure</t> Goat Anti-Rabbit <t>IgG</t> (1:10,000; #111-035-003, Jackson ImmunoResearch Europe Ltd).
    Peroxidase Affinipure Goat Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Jackson Immuno peroxidase affinipure bovine anti goat igg
    Dose-response curves of Ca 2+ uptake in A549 cells and Western blot panel showing CHRNA7 gene silencing. Cells were treated with various concentrations of APS8 ( A ; 10, 5, 2.5, 1, 0.75, 0.5, 0.25, 0.1, 0.05, 0.01 µM) or ACh ( B ; 100, 40, 20, 10, 5, 2.5, 1, 0.5, 0.1, 0.02 µM) or preincubated (20 min, RT) with various concentrations of APS8 and then treated with 100 µM ACh ( C ). The A549 cells were silenced with CHRNA7-targeted or nontargeting (negative control) siRNAs. Data are relative fluorescence units (RFU), as maximum response minus minimum response, divided by the minimum response. The curves and their 95% confidence intervals were obtained by fitting the data with nonlinear regression function (GraphPad Prism). Recombinant human α7 nAChR (#H00001139-P01, Abnova; Lane 1) and lysates of A549 cells treated with nontargeting siRNA (Lane 2) or siRNA targeting CHRNA7 (Lanes 3 and 4) were separated by SDS-PAGE followed by Western blotting ( D ) using rabbit antibodies against human α7 nAChR (1:500; #HPA029422, Sigma-Aldrich) and Peroxidase <t>AffiniPure</t> Goat Anti-Rabbit <t>IgG</t> (1:10,000; #111-035-003, Jackson ImmunoResearch Europe Ltd).
    Peroxidase Affinipure Bovine Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 132 article reviews
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    93
    Jackson Immuno peroxidase affinipure goat anti swine igg
    Dose-response curves of Ca 2+ uptake in A549 cells and Western blot panel showing CHRNA7 gene silencing. Cells were treated with various concentrations of APS8 ( A ; 10, 5, 2.5, 1, 0.75, 0.5, 0.25, 0.1, 0.05, 0.01 µM) or ACh ( B ; 100, 40, 20, 10, 5, 2.5, 1, 0.5, 0.1, 0.02 µM) or preincubated (20 min, RT) with various concentrations of APS8 and then treated with 100 µM ACh ( C ). The A549 cells were silenced with CHRNA7-targeted or nontargeting (negative control) siRNAs. Data are relative fluorescence units (RFU), as maximum response minus minimum response, divided by the minimum response. The curves and their 95% confidence intervals were obtained by fitting the data with nonlinear regression function (GraphPad Prism). Recombinant human α7 nAChR (#H00001139-P01, Abnova; Lane 1) and lysates of A549 cells treated with nontargeting siRNA (Lane 2) or siRNA targeting CHRNA7 (Lanes 3 and 4) were separated by SDS-PAGE followed by Western blotting ( D ) using rabbit antibodies against human α7 nAChR (1:500; #HPA029422, Sigma-Aldrich) and Peroxidase <t>AffiniPure</t> Goat Anti-Rabbit <t>IgG</t> (1:10,000; #111-035-003, Jackson ImmunoResearch Europe Ltd).
    Peroxidase Affinipure Goat Anti Swine Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jackson Immuno peroxidase affinipure donkey anti human igg
    Dose-response curves of Ca 2+ uptake in A549 cells and Western blot panel showing CHRNA7 gene silencing. Cells were treated with various concentrations of APS8 ( A ; 10, 5, 2.5, 1, 0.75, 0.5, 0.25, 0.1, 0.05, 0.01 µM) or ACh ( B ; 100, 40, 20, 10, 5, 2.5, 1, 0.5, 0.1, 0.02 µM) or preincubated (20 min, RT) with various concentrations of APS8 and then treated with 100 µM ACh ( C ). The A549 cells were silenced with CHRNA7-targeted or nontargeting (negative control) siRNAs. Data are relative fluorescence units (RFU), as maximum response minus minimum response, divided by the minimum response. The curves and their 95% confidence intervals were obtained by fitting the data with nonlinear regression function (GraphPad Prism). Recombinant human α7 nAChR (#H00001139-P01, Abnova; Lane 1) and lysates of A549 cells treated with nontargeting siRNA (Lane 2) or siRNA targeting CHRNA7 (Lanes 3 and 4) were separated by SDS-PAGE followed by Western blotting ( D ) using rabbit antibodies against human α7 nAChR (1:500; #HPA029422, Sigma-Aldrich) and Peroxidase <t>AffiniPure</t> Goat Anti-Rabbit <t>IgG</t> (1:10,000; #111-035-003, Jackson ImmunoResearch Europe Ltd).
    Peroxidase Affinipure Donkey Anti Human Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Jackson Immuno peroxidase affinipure mouse anti goat igg
    Dose-response curves of Ca 2+ uptake in A549 cells and Western blot panel showing CHRNA7 gene silencing. Cells were treated with various concentrations of APS8 ( A ; 10, 5, 2.5, 1, 0.75, 0.5, 0.25, 0.1, 0.05, 0.01 µM) or ACh ( B ; 100, 40, 20, 10, 5, 2.5, 1, 0.5, 0.1, 0.02 µM) or preincubated (20 min, RT) with various concentrations of APS8 and then treated with 100 µM ACh ( C ). The A549 cells were silenced with CHRNA7-targeted or nontargeting (negative control) siRNAs. Data are relative fluorescence units (RFU), as maximum response minus minimum response, divided by the minimum response. The curves and their 95% confidence intervals were obtained by fitting the data with nonlinear regression function (GraphPad Prism). Recombinant human α7 nAChR (#H00001139-P01, Abnova; Lane 1) and lysates of A549 cells treated with nontargeting siRNA (Lane 2) or siRNA targeting CHRNA7 (Lanes 3 and 4) were separated by SDS-PAGE followed by Western blotting ( D ) using rabbit antibodies against human α7 nAChR (1:500; #HPA029422, Sigma-Aldrich) and Peroxidase <t>AffiniPure</t> Goat Anti-Rabbit <t>IgG</t> (1:10,000; #111-035-003, Jackson ImmunoResearch Europe Ltd).
    Peroxidase Affinipure Mouse Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Jackson Immuno peroxidase affinipure goat anti mouse igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 3302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jackson Immuno peroxidase affinipure rabbit anti dog igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Rabbit Anti Dog Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno peroxidase affinipure rat anti mouse igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Rat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno peroxidase affinipure mouse anti rabbit igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Mouse Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno peroxidase affinipure donkey anti sheep igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Donkey Anti Sheep Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies peroxidase affinipure goat anti rabbit igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Goat Anti Rabbit Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno peroxidase affinipure goat anti rabbit secondary antibody
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Goat Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology peroxidase affinipure goat anti rabbit igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Goat Anti Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Jackson Immuno peroxidase affinipure goat anti mouse antibody
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Goat Anti Mouse Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Jackson Immuno peroxidase conjugated affinipure f
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Conjugated Affinipure F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Jackson Immuno signaling peroxidase affinipure羊抗鼠igg、peroxidase affinipure羊抗兔igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Signaling Peroxidase Affinipure羊抗鼠igg、Peroxidase Affinipure羊抗兔igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno peroxidase affinipure donkey anti guinea pig igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Donkey Anti Guinea Pig Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno peroxidase affinipure donkey anti rabbit secondary antibody
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Peroxidase Affinipure Donkey Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Dose-response curves of Ca 2+ uptake in A549 cells and Western blot panel showing CHRNA7 gene silencing. Cells were treated with various concentrations of APS8 ( A ; 10, 5, 2.5, 1, 0.75, 0.5, 0.25, 0.1, 0.05, 0.01 µM) or ACh ( B ; 100, 40, 20, 10, 5, 2.5, 1, 0.5, 0.1, 0.02 µM) or preincubated (20 min, RT) with various concentrations of APS8 and then treated with 100 µM ACh ( C ). The A549 cells were silenced with CHRNA7-targeted or nontargeting (negative control) siRNAs. Data are relative fluorescence units (RFU), as maximum response minus minimum response, divided by the minimum response. The curves and their 95% confidence intervals were obtained by fitting the data with nonlinear regression function (GraphPad Prism). Recombinant human α7 nAChR (#H00001139-P01, Abnova; Lane 1) and lysates of A549 cells treated with nontargeting siRNA (Lane 2) or siRNA targeting CHRNA7 (Lanes 3 and 4) were separated by SDS-PAGE followed by Western blotting ( D ) using rabbit antibodies against human α7 nAChR (1:500; #HPA029422, Sigma-Aldrich) and Peroxidase AffiniPure Goat Anti-Rabbit IgG (1:10,000; #111-035-003, Jackson ImmunoResearch Europe Ltd).

    Journal: Marine Drugs

    Article Title: APS8 Delays Tumor Growth in Mice by Inducing Apoptosis of Lung Adenocarcinoma Cells Expressing High Number of α7 Nicotinic Receptors

    doi: 10.3390/md16100367

    Figure Lengend Snippet: Dose-response curves of Ca 2+ uptake in A549 cells and Western blot panel showing CHRNA7 gene silencing. Cells were treated with various concentrations of APS8 ( A ; 10, 5, 2.5, 1, 0.75, 0.5, 0.25, 0.1, 0.05, 0.01 µM) or ACh ( B ; 100, 40, 20, 10, 5, 2.5, 1, 0.5, 0.1, 0.02 µM) or preincubated (20 min, RT) with various concentrations of APS8 and then treated with 100 µM ACh ( C ). The A549 cells were silenced with CHRNA7-targeted or nontargeting (negative control) siRNAs. Data are relative fluorescence units (RFU), as maximum response minus minimum response, divided by the minimum response. The curves and their 95% confidence intervals were obtained by fitting the data with nonlinear regression function (GraphPad Prism). Recombinant human α7 nAChR (#H00001139-P01, Abnova; Lane 1) and lysates of A549 cells treated with nontargeting siRNA (Lane 2) or siRNA targeting CHRNA7 (Lanes 3 and 4) were separated by SDS-PAGE followed by Western blotting ( D ) using rabbit antibodies against human α7 nAChR (1:500; #HPA029422, Sigma-Aldrich) and Peroxidase AffiniPure Goat Anti-Rabbit IgG (1:10,000; #111-035-003, Jackson ImmunoResearch Europe Ltd).

    Article Snippet: This was followed by Western blotting with rabbit antibodies against human α7 nAChR (1:500; #HPA029422, Sigma-Aldrich) and Peroxidase AffiniPure Goat Anti-Rabbit IgG (1:10,000; #111-035-003, Jackson ImmunoResearch Europe Ltd, Ely, Cambridgeshire, UK).

    Techniques: Western Blot, Negative Control, Fluorescence, Recombinant, SDS Page

    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 IgG titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated affiniPure goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .

    Journal: PLoS ONE

    Article Title: Protective Epitopes of the Plasmodium falciparum SERA5 Malaria Vaccine Reside in Intrinsically Unstructured N-Terminal Repetitive Sequences

    doi: 10.1371/journal.pone.0098460

    Figure Lengend Snippet: Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 IgG titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated affiniPure goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .

    Article Snippet: After washing with PBS/T, peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000; or horseradish peroxidase-conjugated rabbit anti-human IgG antibody (A8792; Sigma-Aldrich Corp., St. Louis, MO) diluted 1∶2000; or peroxidase conjugated affiniPure goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000 in 5% skim milk in PBS/T was added to the plates and incubated at 37°C for 1 hour.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation