Journal: PLoS ONE

Article Title: Neutrophils remain detrimentally active in hydroxyurea-treated patients with sickle cell disease

doi: 10.1371/journal.pone.0226583

Figure Lengend Snippet: (A) Purified and rested neutrophils from healthy donors (H) and SCD patients in steady state (SS) were left untreated in RPMI as control or treated with 20 μM hemin. Median Fluorescence Intensity of F-Actin (upper panel) and CD63 (lower panel) in F-Actin/CD63 double positive neutrophils with normal multi-lobulated nuclei is shown. (Healthy N = 7; SCD N = 6). (B) Z-stack stills of SCD neutrophils left in RPMI for 30 minutes showing neutrophils with roughed cell membranes (yellow arrows, upper panel). Staining for DNA (blue), F-Actin (green) and CD63 (red) is presented (lower panel). (C) MPO release was assayed by ELISA following 30 minutes incubation with or without the hemin stimulus (H (Healthy) N = 4; SS (SCD) N = 4). Degranulation response was assayed in terms of MPO ng/ml plasma (upper panel), or fold increase over healthy neutrophils (lower panel). Data presented as dot plots±S.D., significance calculated with an unpaired Mann-Whitney test.

Article Snippet: The supernatants were then assayed with a Human Myeloperoxidase Quantikine ELISA Kit from R&D Systems according to the manufacturer’s instruction.

Techniques: Purification, Control, Fluorescence, Staining, Enzyme-linked Immunosorbent Assay, Incubation, Clinical Proteomics, MANN-WHITNEY

Journal: Biology

Article Title: The Early Growth of Maize Under Waterlogging Stress, as Measured by Growth, Biochemical, and Molecular Characteristics.

doi: 10.3390/biology14020111

Figure Lengend Snippet: Figure 4. Antioxidative enzyme activity in the two genotypes. (A). Ascorbate peroxidase (APX) activity in LT and LS, in all three time points (24 h, 72 h, 7 d). APX activity is shown as APX units/mg of protein. (B). Peroxidase (POD) activity in LT and LS, in all three time points (24 h, 72 h, 7 d). POD activity is shown as POD units/mg of protein. Control samples are shown in orange, and treatment samples are shown in blue. The significance of the difference between the control and treatment of each parameter was determined by t-test and is shown as ** (p < 0.01), * (p < 0.05), and NS (statistically not significant at p > 0.05).

Article Snippet: In alignment with the manufacturer’s instructions, the peroxidase (POD) Activity Assay Kit (Elabscience®, Wuhan, China) was employed for this purpose.

Techniques: Activity Assay, Control

Journal: Cell reports

Article Title: Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5

doi: 10.1016/j.celrep.2023.113145

Figure Lengend Snippet:

Article Snippet: MYC Epitope Tag Antibody Rabbit Polyclonal , Rockland , Cat#600-401-381; RRID:AB_217927.

Techniques: Control, Recombinant, Affinity Purification, Virus, Plasmid Preparation, Quantitative Proteomics, Over Expression, Knock-Out, Software