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Bethyl
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Bethyl
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Proteintech
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Image Search Results
Journal: Scientific Reports
Article Title: MI-181 enhances ciliation and cilia length in a cigarette smoke exposed airway epithelial model
doi: 10.1038/s41598-026-37296-2
Figure Lengend Snippet: Analysis of MI-181’s effect on FOXJ1 levels in multiciliated cells of ABSC ALI cultures exposed to cigarette smoke. ( a-c ) Same as in Fig. a, except ABSC ALI cultures were fixed and co-stained for pericentrin (anti-pericentrin antibody, green) to visualize multiciliated cells and FOXJ1 (anti-FOXJ1, red) to visualize the levels of FOXJ1. The region of interest (ROI) mask used to quantify FOXJ1 levels are in the rightmost panels. Scale bars indicate 5 μm. ( d-f ) Graphs show a summary of the average total relative fluorescence intensity (RFI) for FOXJ1 (y-axis) for each treatment (x-axis), for each of the indicated donors. n = 75 cell images per condition per donor. Data are represented as the average ± SD. Asterisks indicate statistical significance as *** p < 0.001 compared to the controls. See the Quantification and Statistical Analyses section for details.
Article Snippet: Antibodies used for immunofluorescence were anti-acetylated Tubulin (Cat#T6793 Sigma-Aldrich, Burlington, MA, USA, and Cat#ab179484 Abcam, Waltham, MA, USA), anti-FOXJ1 (Cat#14–9965-82 Thermo Fisher Scientific), and
Techniques: Staining, Fluorescence
Journal: Cancer research
Article Title: Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells.
doi: 10.1158/0008-5472.CAN-14-0169
Figure Lengend Snippet: Figure 2. Trim32 is recruited to spindle poles during mitosis through Cdk1/cyclin B phosphorylation signaling. A,representative images of localization of Trim32 to spindle poles during mitosis but not in interphase in SH-SY5Y, TGW, and SK-N-DZ cells. Centrosome marker pericentrin, green; Trim32, red; DAPI (DNA), blue. Arrows, spindle poles. B and C, representative images of Trim32 localization to spindle poles during mitosis in TGW and SK-N-DZ cells upon exposure to 5 mmol/L RO-3306 for 3 hours. DMSO (0.1%)-treated cells were used as a control. Centrosome (centriole) marker centrin-2, is green; Trim32, red; DAPI (DNA), blue. Arrows, spindle poles. D, immunoblot of cyclin B expression in SK-N-DZ cells transfected with control siRNA or Cyclin B siRNA. Immunoblot of b-actin served as a loading control. E, representative images of Trim32 localization to spindle poles during mitosis in SK-N-DZ cells transfected with control siRNA or Cyclin B siRNA. Centrin-2, green; Trim32, red; DAPI (DNA), blue. Arrows, spindle poles. F, percentage of cells with Trim32 localization to spindle poles in SK-N-DZ cells transfected with control siRNA or Cyclin B siRNA. Error bars, SEM from three experiments. Localization statuses of Trim32 at spindle poles were categorized into three types (intense, weak, or no localization). G, representative images of Flag-Trim32 or Flag-Trim32/3A localization to spindle poles during mitosis in SK-N-DZ cells transfected with Flag-Trim32 or Flag-trim32/3A expression vector. Pericentrin, green; Flag, red; DAPI (DNA), blue. Arrows, spindle poles. Flag-Trim32 localizes to spindle poles, but Flag-Trim32/3A does not. Scale bars, 10 mm.
Article Snippet: The primary antibodies used were as follows: antiNuMA antibody (NB500-174; Novus Biologicals), anti-MYCN antibody (sc-53993; Santa Cruz Biotechnology), anti-MYCN antibody (#9405S; Cell Signaling Technology), anti-Trim32 antibody (H00022954-M09; Abnova), anti-Fbxw7 antibody (ab71961; Abcam), anti-Huwe1 antibody (ab70161; Abcam),
Techniques: Phospho-proteomics, Marker, Control, Western Blot, Expressing, Transfection, Plasmid Preparation