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Novus Biologicals
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Santa Cruz Biotechnology
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Biorbyt
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Cyagen Biosciences
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Proteintech
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OriGene
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OriGene
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Addgene inc
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Novus Biologicals
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OriGene
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Santa Cruz Biotechnology
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Image Search Results
Journal: Physiological Reports
Article Title: BMAL1 sex‐specific effects in the neonatal mouse airway exposed to moderate hyperoxia
doi: 10.14814/phy2.16122
Figure Lengend Snippet: Bmal1 WT, Het, and KO protein expression validation. (a) BMAL1 protein expression from Bmal1 WT, Het, and KO male and female neonatal whole lung mouse tissue measured by Jess automated and quantitative immuno‐capillary assay that generates a digital Western blot (representative image shown). ~0.3 mg protein loaded per capillary. BMAL1 peaks were identified using a molecular weight of ~86 kDa. Protein expression was normalized using chemiluminescence of total protein (values indicated on representative image). Ordinary two‐way ANOVA was used to compare Bmal1 genotype and O 2 exposure groups in each sex. Data are represented as mean ± SD; n = 3–9 pups per group. (b) PER1 protein expression using ~0.6 mg protein loaded per capillary based on antibody optimization in Jess. PER1 peaks were identified at a molecular weight of ~50 kDa. Protein expression was normalized using chemiluminescence of total protein (values indicated on representative image). Ordinary two‐way ANOVA was used to compare Bmal1 genotype and O 2 exposure groups in each sex. Data are represented as mean ± SD; n = 3–4 pups per group.
Article Snippet: The following primary antibodies were used: BMAL1 (D2L7G) Rabbit mAb #14020 (Cell Signaling Technology; Danvers, MA, USA) at 1:25;
Techniques: Expressing, Biomarker Discovery, Western Blot, Molecular Weight
Journal: International Journal of General Medicine
Article Title: PER1 Serves as a Tumor Suppressor in Breast Cancer by Regulating MEK5/ERK5 Signaling Pathway
doi: 10.2147/IJGM.S511530
Figure Lengend Snippet: Representative pictures of positive and negative expression of PER1 protein in BC tissues (100× and 400×). ( A ) Positive expression. ( B ) Negative expression.
Article Snippet: The sections were then incubated overnight in primary
Techniques: Expressing
Journal: International Journal of General Medicine
Article Title: PER1 Serves as a Tumor Suppressor in Breast Cancer by Regulating MEK5/ERK5 Signaling Pathway
doi: 10.2147/IJGM.S511530
Figure Lengend Snippet: PER1 affects BC patient’s prognosis. ( A ) RFS and OS of 172 BC patients based on the ten-year follow‑up data. ( B ) RFS and OS in lymph node metastasis subgroup. ( C ) RFS and OS in vascular tumor thrombus subgroup.
Article Snippet: The sections were then incubated overnight in primary
Techniques:
Journal: International Journal of General Medicine
Article Title: PER1 Serves as a Tumor Suppressor in Breast Cancer by Regulating MEK5/ERK5 Signaling Pathway
doi: 10.2147/IJGM.S511530
Figure Lengend Snippet: The key protein expression of the MAPK pathway in the breast cancer cell with low and high PER1 expression. * P <0.05 vs pGenesil-1-KB group; # P <0.05 vs Lenti-blast-KB group.
Article Snippet: The sections were then incubated overnight in primary
Techniques: Expressing
Journal: International Journal of General Medicine
Article Title: PER1 Serves as a Tumor Suppressor in Breast Cancer by Regulating MEK5/ERK5 Signaling Pathway
doi: 10.2147/IJGM.S511530
Figure Lengend Snippet: Effects of ERK5 inhibitor XMD17-109 on MEK5/ERK5 pathway activity. * P <0.05 vs pGenesil-1-KB group; # P <0.05 vs pGenesil-1-PER1 group.
Article Snippet: The sections were then incubated overnight in primary
Techniques: Activity Assay
Journal: International Journal of General Medicine
Article Title: PER1 Serves as a Tumor Suppressor in Breast Cancer by Regulating MEK5/ERK5 Signaling Pathway
doi: 10.2147/IJGM.S511530
Figure Lengend Snippet: XMD17-109 reverses the enhancement of the proliferative ability in BC cells with low expression of PER1 (100×). ( A ) Colony Formation Assay. ( B ) EdU Assay. * P <0.05 vs pGenesil-1-KB group; # P <0.05 vs pGenesil-1-PER1 group.
Article Snippet: The sections were then incubated overnight in primary
Techniques: Expressing, Colony Assay, EdU Assay
Journal: International Journal of General Medicine
Article Title: PER1 Serves as a Tumor Suppressor in Breast Cancer by Regulating MEK5/ERK5 Signaling Pathway
doi: 10.2147/IJGM.S511530
Figure Lengend Snippet: XMD17-109 reverses the increased migration and invasion ability in BC cells with low expression of PER1 (100×). ( A ) Transwell tumor cell migration assay. ( B ) Transwell tumor cell invasion assay. * P <0.05 vs pGenesil-1-KB group; # P <0.05 vs pGenesil-1-PER1 group.
Article Snippet: The sections were then incubated overnight in primary
Techniques: Migration, Expressing, Cell Migration Assay, Invasion Assay