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Genencor Inc
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US Biological Life Sciences
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Image Search Results
Journal: The Journal of pathology
Article Title: Bmi1 marks gastric stem cells located in the isthmus in mice.
doi: 10.1002/path.5244
Figure Lengend Snippet: Figure 1. Bmi1-expressing cells were mainly located in the isthmus of the stomach and were unique from other differentiated or stem cells. (A) Immunohistochemistry for BMI1 in the normal human gastric epithelium. The location of BMI1-positive cells and the distribution ratio in the human gastric epithelium were plotted (n = 3). Scale bars: 50 μm. (B) Immunohistochemistry for GFP in 8-week-old Bmi1-CreERT; Rosa26-YFP mice 1 day after 0.1 mg/g body weight tamoxifen injection. Bar graphs show the distribution of Bmi1+/YFP+ cells in the gastric epithelium (n = 3). Scale bars: 10 μm. (C) Immunofluorescent double staining for GFP and gastric differentiated cell markers: Muc5AC, chromogranin A (ChgA), Dclk1, Tff2, proton pump (PP) and pepsinogen1 (Pga1) in 8-week-old Bmi1-CreERT; Rosa26-YFP mice 1 day after 0.1 mg/g body weight tamoxifen injection. Scale bars: 10 μm. (D) Immunofluorescent double staining for Bmi1 and GFP on day 1 after 0.1 mg/g body weight tamoxifen injection in Lgr5-EGFP-CreERT mice and eR1-CreERT; Rosa26-YFP mice. Scale bars: 10 μm.
Article Snippet: Primary antibodies were obtained from the indicated suppliers: goat anti-GFP (×500; ab6673, Abcam, Cambridge, MA, USA), rat anti-Ki67 (×100; M7249, Dako), anti-Muc5AC (×100; ab3649, Abcam), rabbit anti-chromogranin A (×100; ab15160, Abcam), rabbit anti-Dclk1 (×200; ab31704, Abcam), mouse anti-spasmolytic polypeptide IgM (×100; ab49536, Abcam), mouse anti-proton pump (×1; D031-3H, Medical & Biological Laboratories, Nagoya, Aichi, Japan),
Techniques: Expressing, Immunohistochemistry, Injection, Double Staining