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    An entry vector carrying the yeast constitutive promoter TR SSRE 730 bp flanked by two att sites att L4 and att R1 This promoter is part of the ATCC Synthetic
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    99
    Thermo Fisher pentr d topo
    Pentr D Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 12462 article reviews
    Price from $9.99 to $1999.99
    pentr d topo - by Bioz Stars, 2021-01
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    99
    Thermo Fisher pentr vector
    Pentr Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr vector/product/Thermo Fisher
    Average 99 stars, based on 2453 article reviews
    Price from $9.99 to $1999.99
    pentr vector - by Bioz Stars, 2021-01
    99/100 stars
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    91
    Thermo Fisher pentr directional topo cloning kit
    Pentr Directional Topo Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1240 article reviews
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    pentr directional topo cloning kit - by Bioz Stars, 2021-01
    91/100 stars
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    99
    Thermo Fisher pentr sd d topo vector
    Pentr Sd D Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr sd d topo vector/product/Thermo Fisher
    Average 99 stars, based on 861 article reviews
    Price from $9.99 to $1999.99
    pentr sd d topo vector - by Bioz Stars, 2021-01
    99/100 stars
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    89
    Thermo Fisher pentr topo vector
    Pentr Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr topo vector/product/Thermo Fisher
    Average 89 stars, based on 415 article reviews
    Price from $9.99 to $1999.99
    pentr topo vector - by Bioz Stars, 2021-01
    89/100 stars
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    89
    Thermo Fisher pentr u6 vector
    Pentr U6 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr u6 vector/product/Thermo Fisher
    Average 89 stars, based on 632 article reviews
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    pentr u6 vector - by Bioz Stars, 2021-01
    89/100 stars
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    99
    Thermo Fisher pentr 1a vector
    Pentr 1a Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr 1a vector/product/Thermo Fisher
    Average 99 stars, based on 323 article reviews
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    pentr 1a vector - by Bioz Stars, 2021-01
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    94
    Addgene inc e cadherin
    Cx43 directly interacts with PCDH7, but not with E <t>cadherin</t> or N cadherin a, Schema illustrating split luciferase assay. Fusion constructs of PCDH7 and Cx43 were created with either the N or C terminal halves of luciferase, NLuc and CLuc, respectively. When these proteins are brought into proximity, luciferase is functionally reconstituted, producing photons of light. b, Cx43 and PCDH7 constructs fused with N-terminal and C-terminal firefly luciferase halves (N-luc) and (C-luc) were expressed in parental cell lines. The table (top) numerically identifies the cell line combinations used in the assays (bottom), and bioluminescence imaging (BLI) of a representative plate. c , Cx43 and PCDH7 western immunoblotting in cancer cells overexpressing fusion proteins. d, Quantification of BLI after co-culture of Cx43-CLuc/PCDH7-NLuc(+) cancer cells and astrocytes for 15 min. (3 independent experiments) e-g, Luciferase split assay to detect Cx43-E cadherin or Cx43-N cadherin interactions. Cell line combinations used in the assays are numerically identified in the table ( e ), and confirmed by western immunoblotting ( f ). Bioluminescence imaging (BLI) of a representative assay plate; cell line combinations are indicated numerically ( g ). (n ≥ 2 independent experiments).
    E Cadherin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Addgene inc
    Average 94 stars, based on 141 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2021-01
    94/100 stars
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    Centralized Validation
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    N/A
    Standard format Plasmid sent in bacteria as agar stab
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    Image Search Results


    Cx43 directly interacts with PCDH7, but not with E cadherin or N cadherin a, Schema illustrating split luciferase assay. Fusion constructs of PCDH7 and Cx43 were created with either the N or C terminal halves of luciferase, NLuc and CLuc, respectively. When these proteins are brought into proximity, luciferase is functionally reconstituted, producing photons of light. b, Cx43 and PCDH7 constructs fused with N-terminal and C-terminal firefly luciferase halves (N-luc) and (C-luc) were expressed in parental cell lines. The table (top) numerically identifies the cell line combinations used in the assays (bottom), and bioluminescence imaging (BLI) of a representative plate. c , Cx43 and PCDH7 western immunoblotting in cancer cells overexpressing fusion proteins. d, Quantification of BLI after co-culture of Cx43-CLuc/PCDH7-NLuc(+) cancer cells and astrocytes for 15 min. (3 independent experiments) e-g, Luciferase split assay to detect Cx43-E cadherin or Cx43-N cadherin interactions. Cell line combinations used in the assays are numerically identified in the table ( e ), and confirmed by western immunoblotting ( f ). Bioluminescence imaging (BLI) of a representative assay plate; cell line combinations are indicated numerically ( g ). (n ≥ 2 independent experiments).

    Journal: Nature

    Article Title: Carcinoma-astrocyte gap junctions promote brain metastasis by cGAMP transfer

    doi: 10.1038/nature18268

    Figure Lengend Snippet: Cx43 directly interacts with PCDH7, but not with E cadherin or N cadherin a, Schema illustrating split luciferase assay. Fusion constructs of PCDH7 and Cx43 were created with either the N or C terminal halves of luciferase, NLuc and CLuc, respectively. When these proteins are brought into proximity, luciferase is functionally reconstituted, producing photons of light. b, Cx43 and PCDH7 constructs fused with N-terminal and C-terminal firefly luciferase halves (N-luc) and (C-luc) were expressed in parental cell lines. The table (top) numerically identifies the cell line combinations used in the assays (bottom), and bioluminescence imaging (BLI) of a representative plate. c , Cx43 and PCDH7 western immunoblotting in cancer cells overexpressing fusion proteins. d, Quantification of BLI after co-culture of Cx43-CLuc/PCDH7-NLuc(+) cancer cells and astrocytes for 15 min. (3 independent experiments) e-g, Luciferase split assay to detect Cx43-E cadherin or Cx43-N cadherin interactions. Cell line combinations used in the assays are numerically identified in the table ( e ), and confirmed by western immunoblotting ( f ). Bioluminescence imaging (BLI) of a representative assay plate; cell line combinations are indicated numerically ( g ). (n ≥ 2 independent experiments).

    Article Snippet: Split luciferase assay Fusion cDNAs were generated by deleting the stop codon in human Cx43 (Origene), PCDH7 (Origene), E-cadherin (Addgene) or N-cadherin (Addgene) cDNAs and splicing the N-terminal or C-terminal fragment of firefly luciferase . (Addgene).

    Techniques: Luciferase, Construct, Imaging, Western Blot, Co-Culture Assay