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  • 93
    Lonza penicillin streptomycin
    Penicillin Streptomycin, supplied by Lonza, used in various techniques. Bioz Stars score: 93/100, based on 5972 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC penicillin streptomycin
    Penicillin Streptomycin, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher penicillin streptomycin
    Penicillin Streptomycin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 156248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore penicillin streptomycin
    Penicillin Streptomycin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 36709 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher penicillin
    Penicillin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 35324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore penicillin
    Penicillin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14769 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare penicillin streptomycin
    Penicillin Streptomycin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 7916 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher penicillin g
    Penicillin G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher penicillin streptomycin solution
    Penicillin Streptomycin Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6511 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore penicillin g
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Penicillin G, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare streptomycin
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Streptomycin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 5430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher penicillin streptomycin glutamine
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Penicillin Streptomycin Glutamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech penicillin streptomycin
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Penicillin Streptomycin, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 4162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences penicillin streptomycin
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Penicillin Streptomycin, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 3159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore penicillin streptomycin solution
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Penicillin Streptomycin Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3015 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Cellgro penicillin streptomycin
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Penicillin Streptomycin, supplied by Cellgro, used in various techniques. Bioz Stars score: 94/100, based on 3015 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biochrom penicillin streptomycin
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Penicillin Streptomycin, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 2983 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PAA Laboratories penicillin streptomycin
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
    Penicillin Streptomycin, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 2082 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher streptomycin penicillin
    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of <t>penicillin</t> G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001
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    Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of penicillin G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001

    Journal: Nature Communications

    Article Title: Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome

    doi: 10.1038/s41467-018-03143-w

    Figure Lengend Snippet: Optimising a synV host by SCRaMbLE for enhanced violacein production. a The workflow of a SCRaMbLE host optimisation process. b Eighty seven post-SCRaMbLE synV-pJCH017 colonies and synV, synV-pRS416 and synV-pJCH017 controls were grown in liquid culture, spotted onto SDO URA − agar medium and grown for 2 days. The best performing strain, VB2, is highlighted. c Results of colourimetric determination of violacein yields from synV-pJCH017, VB2-pJCH017 and VB2c-pJCH017 cultures, n = 3. Inset are microtubes in which equal volumes of synV-pJCH017 and VB2-pJCH017 culture, normalised for OD 700 , have been pelleted by centrifugation. d sfGFP production rates from synV-pBAB012 and VB2-pBAB012 cultures, n ≥ 7. Data shown are the increase in 520 nm fluorescence value over the previous hour normalised to the OD 600 value at the midpoint of that hour. e The 520 nm fluorescence levels of cultures of synV and VB2 strains containing pBAB011 ( CEN6 / URA3 ), pBAB012 (2μ/ URA3 ), pBAB015 ( CEN6 / LEU2 ) or pBAB016 (2μ/ LEU2 ) plasmids after 29.5 h of growth. Fluorescence values are normalised for OD 600 , n ≥ 7. f qPCR evaluation of pJCH017 copy number in total DNA preparations from stationary synV-pJCH017 and VB2-pJCH017 cultures. The vioD , vioB and kanR targets are located on pJCH017 and 'all' represents the combined data from amplification of these three loci. Relative copy number is the calculated concentration of pJCH017 in the total DNA for each experiment normalised to the combined synV-pJCH017 value. The ACT1 control shows the threshold cycle for amplification of a chromosomal ACT1 target for each DNA preparation, demonstrating equal levels of genomic DNA, n = 6 (2 technical replicates each of 3 biological replicates). g The amount of penicillin G secreted by BY4741-pAA152/171, synV-pAA152/171 and VB2-pAA152/171 strains as determined by LC-MS. Values are normalised for OD 600 , n = 3. All values plotted are mean averages and error bars represent 1 standard deviation from the mean. Replicate numbers represent biological replicates except where otherwise stated. Asterisks denote two-tail p -value as determined by two-sample t -test, with * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001

    Article Snippet: Penicillin G (Sigma Aldrich) standards of concentration 0 ng ml−1 , 10 ng ml−1 , 100 ng ml−1 , 1 μg ml−1 and 10 μg ml−1 were used to construct a curve of best fit that was then used to quantify Penicillin G in culture supernatant samples.

    Techniques: Centrifugation, Fluorescence, Real-time Polymerase Chain Reaction, Amplification, Concentration Assay, Liquid Chromatography with Mass Spectroscopy, Standard Deviation