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insertion at tigre  (Addgene inc)
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Addgene inc insertion at tigre
a , Targeted <t>homozygous</t> <t>insertion</t> of CAS9 and OsTIR1 F74G at the <t>TIGRE</t> locus (left) results in constitutive CAS9 and TIR1 protein expression as assessed by western blot (right). b , Schematic representation of homozygous knockin of GFP-AID at (and in frame with) the start codon (N-terminus) of endogenous Brca1 or Brca2 , and PCR-genotyping of the resulting BRCA1- and BRCA2-degron cell lines. c , Venn diagram representation of total synthetic lethal and viable interactions scored in the BRCA1- and BRCA2-screens (FDR<0.05 and RRA<0.05). d , Top 10 Reactome pathways enriched among total BRCA1- and BRCA2- screen hits, ranked by signal (FDR-adjusted p-values with Benjamini-Hochberg correction). e , Dotplot representation of genes targeted by the genome-wide gRNA library ranked according to their synthetic viability (top) or synthetic lethality (bottom) scores across three different timepoints in the BRCA1-degron and f , BRCA2-degron screens. Statistically significant synthetic viable and lethal genes are shown as blue and red dots, respectively. A few top genes are labeled.
Insertion At Tigre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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insertion at tigre - by Bioz Stars, 2026-05
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a , Targeted homozygous insertion of CAS9 and OsTIR1 F74G at the TIGRE locus (left) results in constitutive CAS9 and TIR1 protein expression as assessed by western blot (right). b , Schematic representation of homozygous knockin of GFP-AID at (and in frame with) the start codon (N-terminus) of endogenous Brca1 or Brca2 , and PCR-genotyping of the resulting BRCA1- and BRCA2-degron cell lines. c , Venn diagram representation of total synthetic lethal and viable interactions scored in the BRCA1- and BRCA2-screens (FDR<0.05 and RRA<0.05). d , Top 10 Reactome pathways enriched among total BRCA1- and BRCA2- screen hits, ranked by signal (FDR-adjusted p-values with Benjamini-Hochberg correction). e , Dotplot representation of genes targeted by the genome-wide gRNA library ranked according to their synthetic viability (top) or synthetic lethality (bottom) scores across three different timepoints in the BRCA1-degron and f , BRCA2-degron screens. Statistically significant synthetic viable and lethal genes are shown as blue and red dots, respectively. A few top genes are labeled.

Journal: bioRxiv

Article Title: The FANCM-RMI1/2 complex promotes genomic instability and PARP inhibitor sensitivity in BRCA2-deficicient cells

doi: 10.64898/2026.01.15.699681

Figure Lengend Snippet: a , Targeted homozygous insertion of CAS9 and OsTIR1 F74G at the TIGRE locus (left) results in constitutive CAS9 and TIR1 protein expression as assessed by western blot (right). b , Schematic representation of homozygous knockin of GFP-AID at (and in frame with) the start codon (N-terminus) of endogenous Brca1 or Brca2 , and PCR-genotyping of the resulting BRCA1- and BRCA2-degron cell lines. c , Venn diagram representation of total synthetic lethal and viable interactions scored in the BRCA1- and BRCA2-screens (FDR<0.05 and RRA<0.05). d , Top 10 Reactome pathways enriched among total BRCA1- and BRCA2- screen hits, ranked by signal (FDR-adjusted p-values with Benjamini-Hochberg correction). e , Dotplot representation of genes targeted by the genome-wide gRNA library ranked according to their synthetic viability (top) or synthetic lethality (bottom) scores across three different timepoints in the BRCA1-degron and f , BRCA2-degron screens. Statistically significant synthetic viable and lethal genes are shown as blue and red dots, respectively. A few top genes are labeled.

Article Snippet: The plasmid to simultaneously target CAS9 and OsTIR1(F74G variant) at the TIGRE locus (pFD155) was engineered by cloning Cas9-t2a-hygro and OsTir1(F74G)-V5 cassettes under pgk and CAG promoters respectively, into a plasmid containing 5’ and 3’ homology arms for targeted insertion at TIGRE (Addgene plasmid 92141).

Techniques: Expressing, Western Blot, Knock-In, Genome Wide, Labeling