Journal: The Journal of Biological Chemistry

Article Title: Clustered Kv2.1 decreases dopamine transporter activity and internalization

doi: 10.1074/jbc.RA119.007441

Figure Lengend Snippet: Plasmid constructs EYFP is enhanced yellow fluorescent protein.

Article Snippet: A summary of all chemicals, drugs, and antibodies used in this study can be found in in – . table ft1 table-wrap mode="anchored" t5 caption a7 External and internal solution constituents Open in a separate window table ft1 table-wrap mode="anchored" t5 caption a7 Primary culture solution constituents Open in a separate window table ft1 table-wrap mode="anchored" t5 caption a7 Protein Fluorophore Vector Source Kv2.1 GFP RBG4 Trimmer ( 25 ) s586a–Kv2.1 GFP RBG4 Trimmer ( 38 ) Kv2.1 mCerulean3 mCerulean3-C1 M. Davidson (Vector Labs and Addgene) DAT Tag RFP pTagRFP–T-C1 Melikian ( 55 ) DAT EYFP stable expressing (pEYFP-N1) Javitch ( 41 ) Transferrin receptor mCerulean mCerulean M. Davidson (Addgene) E YFP EYFP pEYFP–N1 Clontech mCerulean3 mCerulean3 mCerulean3-C1 M. Davidson (Addgene) FRET8 mCerulean/mVenus pEGFP-C1 Piston ( 56 ) EEA1 Tag RFP pcDNA3 S. Corvera (Addgene) Open in a separate window Plasmid constructs EYFP is enhanced yellow fluorescent protein.

Techniques: Plasmid Preparation, Construct, Expressing