pecy5 Search Results


pecy5 conjugated anti-cd19  (Proimmune)
90
Proimmune pecy5 conjugated anti-cd19
Pecy5 Conjugated Anti Cd19, supplied by Proimmune, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pecy5 conjugated anti-cd19/product/Proimmune
Average 90 stars, based on 1 article reviews
pecy5 conjugated anti-cd19 - by Bioz Stars, 2026-04
90/100 stars
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cd5 (pecy5  (Immunostep)
90
Immunostep cd5 (pecy5
Cd5 (Pecy5, supplied by Immunostep, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd5 (pecy5/product/Immunostep
Average 90 stars, based on 1 article reviews
cd5 (pecy5 - by Bioz Stars, 2026-04
90/100 stars
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cd107a pecy5  (Lifetech Scientific Corporation)
90
Lifetech Scientific Corporation cd107a pecy5
CE-XTC manufacturing induces CE Env- and CE Gag-specific CD4+ and CD8+ T cells expressing one or more effector functions. Pre-expansion PBMC (circles) and post-expansion CE-XTCs (triangles) from SHIV-infected, ART suppressed donors (closed shapes, n = 3) and uninfected donors (open shapes, n = 2) were expanded for 19 days and then stimulated overnight with CE peptide pools comprising either 7 regions of SIV Gag (A, C) or 12 regions of HIV Env (B, D) . Following peptide stimulation, effector functions were analyzed by intracellular cytokine staining and flow cytometry. Shown are the increases in frequencies of CD4 and CD8 T cells expressing one or more of the cytokines IFN-γ, IL-2, TNFα or co-expression of <t>CD107a</t> and Granzyme B as markers of cytolytic effector function after the 19-day expansion period. P values comparing responses before and after expansion are shown for each individual effector function (A, B) or as polyfunctional responses (C, D) defined as cells expressing 1 or more cytokine and/or cytolytic effector functions. Individual responses were compared by Mixed-effects model (REML) adjusted for multiple comparisons by Sidak’s multiple comparison test (A, B) * = p<0.05. Differences in paired pre- vs. post-expansion T-cell response polyfunctionality were compared by Wilcoxon matched-pairs signed rank test (C, D) .
Cd107a Pecy5, supplied by Lifetech Scientific Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd107a pecy5/product/Lifetech Scientific Corporation
Average 90 stars, based on 1 article reviews
cd107a pecy5 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
pecy5-cd4  (Cytopeia Inc)
90
Cytopeia Inc pecy5-cd4
CE-XTC manufacturing induces CE Env- and CE Gag-specific CD4+ and CD8+ T cells expressing one or more effector functions. Pre-expansion PBMC (circles) and post-expansion CE-XTCs (triangles) from SHIV-infected, ART suppressed donors (closed shapes, n = 3) and uninfected donors (open shapes, n = 2) were expanded for 19 days and then stimulated overnight with CE peptide pools comprising either 7 regions of SIV Gag (A, C) or 12 regions of HIV Env (B, D) . Following peptide stimulation, effector functions were analyzed by intracellular cytokine staining and flow cytometry. Shown are the increases in frequencies of CD4 and CD8 T cells expressing one or more of the cytokines IFN-γ, IL-2, TNFα or co-expression of <t>CD107a</t> and Granzyme B as markers of cytolytic effector function after the 19-day expansion period. P values comparing responses before and after expansion are shown for each individual effector function (A, B) or as polyfunctional responses (C, D) defined as cells expressing 1 or more cytokine and/or cytolytic effector functions. Individual responses were compared by Mixed-effects model (REML) adjusted for multiple comparisons by Sidak’s multiple comparison test (A, B) * = p<0.05. Differences in paired pre- vs. post-expansion T-cell response polyfunctionality were compared by Wilcoxon matched-pairs signed rank test (C, D) .
Pecy5 Cd4, supplied by Cytopeia Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pecy5-cd4/product/Cytopeia Inc
Average 90 stars, based on 1 article reviews
pecy5-cd4 - by Bioz Stars, 2026-04
90/100 stars
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phycoerythrin-cyanine 5 (pecy5)-conjugated anti-cd4  (GeneMay Inc)
90
GeneMay Inc phycoerythrin-cyanine 5 (pecy5)-conjugated anti-cd4
CE-XTC manufacturing induces CE Env- and CE Gag-specific CD4+ and CD8+ T cells expressing one or more effector functions. Pre-expansion PBMC (circles) and post-expansion CE-XTCs (triangles) from SHIV-infected, ART suppressed donors (closed shapes, n = 3) and uninfected donors (open shapes, n = 2) were expanded for 19 days and then stimulated overnight with CE peptide pools comprising either 7 regions of SIV Gag (A, C) or 12 regions of HIV Env (B, D) . Following peptide stimulation, effector functions were analyzed by intracellular cytokine staining and flow cytometry. Shown are the increases in frequencies of CD4 and CD8 T cells expressing one or more of the cytokines IFN-γ, IL-2, TNFα or co-expression of <t>CD107a</t> and Granzyme B as markers of cytolytic effector function after the 19-day expansion period. P values comparing responses before and after expansion are shown for each individual effector function (A, B) or as polyfunctional responses (C, D) defined as cells expressing 1 or more cytokine and/or cytolytic effector functions. Individual responses were compared by Mixed-effects model (REML) adjusted for multiple comparisons by Sidak’s multiple comparison test (A, B) * = p<0.05. Differences in paired pre- vs. post-expansion T-cell response polyfunctionality were compared by Wilcoxon matched-pairs signed rank test (C, D) .
Phycoerythrin Cyanine 5 (Pecy5) Conjugated Anti Cd4, supplied by GeneMay Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phycoerythrin-cyanine 5 (pecy5)-conjugated anti-cd4/product/GeneMay Inc
Average 90 stars, based on 1 article reviews
phycoerythrin-cyanine 5 (pecy5)-conjugated anti-cd4 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


CE-XTC manufacturing induces CE Env- and CE Gag-specific CD4+ and CD8+ T cells expressing one or more effector functions. Pre-expansion PBMC (circles) and post-expansion CE-XTCs (triangles) from SHIV-infected, ART suppressed donors (closed shapes, n = 3) and uninfected donors (open shapes, n = 2) were expanded for 19 days and then stimulated overnight with CE peptide pools comprising either 7 regions of SIV Gag (A, C) or 12 regions of HIV Env (B, D) . Following peptide stimulation, effector functions were analyzed by intracellular cytokine staining and flow cytometry. Shown are the increases in frequencies of CD4 and CD8 T cells expressing one or more of the cytokines IFN-γ, IL-2, TNFα or co-expression of CD107a and Granzyme B as markers of cytolytic effector function after the 19-day expansion period. P values comparing responses before and after expansion are shown for each individual effector function (A, B) or as polyfunctional responses (C, D) defined as cells expressing 1 or more cytokine and/or cytolytic effector functions. Individual responses were compared by Mixed-effects model (REML) adjusted for multiple comparisons by Sidak’s multiple comparison test (A, B) * = p<0.05. Differences in paired pre- vs. post-expansion T-cell response polyfunctionality were compared by Wilcoxon matched-pairs signed rank test (C, D) .

Journal: Frontiers in Immunology

Article Title: Efficient ex vivo expansion of conserved element vaccine-specific CD8+ T-cells from SHIV-infected, ART-suppressed nonhuman primates

doi: 10.3389/fimmu.2023.1188018

Figure Lengend Snippet: CE-XTC manufacturing induces CE Env- and CE Gag-specific CD4+ and CD8+ T cells expressing one or more effector functions. Pre-expansion PBMC (circles) and post-expansion CE-XTCs (triangles) from SHIV-infected, ART suppressed donors (closed shapes, n = 3) and uninfected donors (open shapes, n = 2) were expanded for 19 days and then stimulated overnight with CE peptide pools comprising either 7 regions of SIV Gag (A, C) or 12 regions of HIV Env (B, D) . Following peptide stimulation, effector functions were analyzed by intracellular cytokine staining and flow cytometry. Shown are the increases in frequencies of CD4 and CD8 T cells expressing one or more of the cytokines IFN-γ, IL-2, TNFα or co-expression of CD107a and Granzyme B as markers of cytolytic effector function after the 19-day expansion period. P values comparing responses before and after expansion are shown for each individual effector function (A, B) or as polyfunctional responses (C, D) defined as cells expressing 1 or more cytokine and/or cytolytic effector functions. Individual responses were compared by Mixed-effects model (REML) adjusted for multiple comparisons by Sidak’s multiple comparison test (A, B) * = p<0.05. Differences in paired pre- vs. post-expansion T-cell response polyfunctionality were compared by Wilcoxon matched-pairs signed rank test (C, D) .

Article Snippet: Cells were surface stained with CD45 PECF594 (d058-1283, BD), LIVE/Dead Aqua (ThermoFisher), CD3 BV650 (Sp34-2, BD), CD4 BV605 (OKT4, BioLegend), CD8 (RPA-T8, BioLegend), CD28 BV711 (CD28.2, BioLegend), and CD95 APC-Cy7 (DX2, BioLegend), then permeabilized and fixed using CytoFix/Perm kit (BD Biosciences) then intracellularly stained (ICS) with IL-2 AF700 (MQ1-17H12, BioLegend), TNFα Pe-Cy7 (Mab11, BD), granzyme B BV421 (GB11, BD), and CD107a PeCy5 (eBioH4A3, LifeTech) and either IFNɣ FITC (B27, BD) prior to CE-XTC infusion or IFNɣ BB700 (B27, BD) post CE-XTC infusion, to allow detection of CFSE-labeled CE-XTCs in the DMSO negative control at acquisition.

Techniques: Expressing, Infection, Staining, Flow Cytometry

Magnitude and functionality of expanded CE Env- and CE Gag-specific CD4 + and CD8 + T-cell responses. PBMC or CE-XTCs from all 5 animals were stimulated overnight prior to their expansion and at day 19 post-expansion with overlapping peptide pools representing sequences for each of 7 or 12 conserved elements of SIV Gag and HIV Env, respectively, and their frequencies were measured by flow cytometry following cell surface and intracellular cytokine staining. Shown are the cumulative frequencies of stimulated CD4 + (A, C) and CD8 + (B, D) Gag and Env CE-specific T cells expressing IFN-γ, IL-2, TNFα and/or CD107a with granzyme B at pre- (A, B) and 19 days post-expansion (C, D) . Median 1, 2, 3 and 4 function CE-specific CD4 (E) and CD8 (F) T cells were compared by mixed-effects model (REML) adjusted for multiple comparisons by Sidak’s multiple comparison test. P <0.05 = *, P <0.01 = **, P < 0.001 = ***, P < 0.0001 = ****.

Journal: Frontiers in Immunology

Article Title: Efficient ex vivo expansion of conserved element vaccine-specific CD8+ T-cells from SHIV-infected, ART-suppressed nonhuman primates

doi: 10.3389/fimmu.2023.1188018

Figure Lengend Snippet: Magnitude and functionality of expanded CE Env- and CE Gag-specific CD4 + and CD8 + T-cell responses. PBMC or CE-XTCs from all 5 animals were stimulated overnight prior to their expansion and at day 19 post-expansion with overlapping peptide pools representing sequences for each of 7 or 12 conserved elements of SIV Gag and HIV Env, respectively, and their frequencies were measured by flow cytometry following cell surface and intracellular cytokine staining. Shown are the cumulative frequencies of stimulated CD4 + (A, C) and CD8 + (B, D) Gag and Env CE-specific T cells expressing IFN-γ, IL-2, TNFα and/or CD107a with granzyme B at pre- (A, B) and 19 days post-expansion (C, D) . Median 1, 2, 3 and 4 function CE-specific CD4 (E) and CD8 (F) T cells were compared by mixed-effects model (REML) adjusted for multiple comparisons by Sidak’s multiple comparison test. P <0.05 = *, P <0.01 = **, P < 0.001 = ***, P < 0.0001 = ****.

Article Snippet: Cells were surface stained with CD45 PECF594 (d058-1283, BD), LIVE/Dead Aqua (ThermoFisher), CD3 BV650 (Sp34-2, BD), CD4 BV605 (OKT4, BioLegend), CD8 (RPA-T8, BioLegend), CD28 BV711 (CD28.2, BioLegend), and CD95 APC-Cy7 (DX2, BioLegend), then permeabilized and fixed using CytoFix/Perm kit (BD Biosciences) then intracellularly stained (ICS) with IL-2 AF700 (MQ1-17H12, BioLegend), TNFα Pe-Cy7 (Mab11, BD), granzyme B BV421 (GB11, BD), and CD107a PeCy5 (eBioH4A3, LifeTech) and either IFNɣ FITC (B27, BD) prior to CE-XTC infusion or IFNɣ BB700 (B27, BD) post CE-XTC infusion, to allow detection of CFSE-labeled CE-XTCs in the DMSO negative control at acquisition.

Techniques: Flow Cytometry, Staining, Expressing