pearson t test Search Results


pearson chi-squared test  (CH Instruments)
90
CH Instruments pearson chi-squared test
Univariate analysis of C2 E318D genotype with in-hospital mortality. : Individuals with the GA genotype appeared to have a higher mortality rate than the GG genotype category. The p-value for this using <t> Pearson's </t> chi-square test was 0.05. The AA genotype was excluded from the analysis because only a single individual had this genotype
Pearson Chi Squared Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/pearson+chi+square+test/pmc02697907-137-19-20
Average 90 stars, based on 1 article reviews
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independence test (pearson’s chi-square)  (CH Instruments)
90
CH Instruments independence test (pearson’s chi-square)
Univariate analysis of C2 E318D genotype with in-hospital mortality. : Individuals with the GA genotype appeared to have a higher mortality rate than the GG genotype category. The p-value for this using <t> Pearson's </t> chi-square test was 0.05. The AA genotype was excluded from the analysis because only a single individual had this genotype
Independence Test (Pearson’s Chi Square), supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/2+test+of+independence/pm36541947-75-10-13
Average 90 stars, based on 1 article reviews
independence test (pearson’s chi-square) - by Bioz Stars, 2026-09
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chi-square test  (CH Instruments)
90
CH Instruments chi-square test
Univariate analysis of C2 E318D genotype with in-hospital mortality. : Individuals with the GA genotype appeared to have a higher mortality rate than the GG genotype category. The p-value for this using <t> Pearson's </t> chi-square test was 0.05. The AA genotype was excluded from the analysis because only a single individual had this genotype
Chi Square Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/chi+square+test/pm17577080-53-50-49
Average 90 stars, based on 1 article reviews
chi-square test - by Bioz Stars, 2026-09
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chi-square test of pearson 2  (CH Instruments)
90
CH Instruments chi-square test of pearson 2
Univariate analysis of C2 E318D genotype with in-hospital mortality. : Individuals with the GA genotype appeared to have a higher mortality rate than the GG genotype category. The p-value for this using <t> Pearson's </t> chi-square test was 0.05. The AA genotype was excluded from the analysis because only a single individual had this genotype
Chi Square Test Of Pearson 2, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/pearson+2+sided+asymptotic+chi+squared+test/pmc08608912-195-8-5
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chi-square test of pearson 2 - by Bioz Stars, 2026-09
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pearson chi-square association test  (CH Instruments)
90
CH Instruments pearson chi-square association test
Univariate analysis of C2 E318D genotype with in-hospital mortality. : Individuals with the GA genotype appeared to have a higher mortality rate than the GG genotype category. The p-value for this using <t> Pearson's </t> chi-square test was 0.05. The AA genotype was excluded from the analysis because only a single individual had this genotype
Pearson Chi Square Association Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/pearson+chi+square+test+of+association/pmc08225323-167-21-22
Average 90 stars, based on 1 article reviews
pearson chi-square association test - by Bioz Stars, 2026-09
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two-sided pearson chi-squared tests  (CH Instruments)
90
CH Instruments two-sided pearson chi-squared tests
ACTN2 is a direct target gene of the NF-κB signaling in GC cells. A c-Fos, NFATc1, RelA, Smad3 or Smad4 was cotransfacted with the ACTN2 promoter (-1918/ + 2455) reporter gene ( ACTN2 Luc-R) into SNU-16 cells for 12 h and dual-luciferase reporter assays were performed. B ACTN2 promoter 5′ sequential deletion constructs. Fragments of different lengths of the ACTN2 promoter with the same 3′ end were cloned into pGL3-Basic. RelA was co-transfected with each of the ACTN2 promoter-based reporters for 12 h and dual-luciferase reporter assays were performed. C Mutation of putative RelA binding site at the ACTN2 promoter. SNU-16 cells were transfected with wild type or mutant ACTN2 Luc-R for 12 h and dual-luciferase reporter assays were performed. The statistical analysis information in A-C: The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Ordinary one-way ANOVA. D NAGC and HAGC tissue sections obtained after infusion were immunostained for α-Actinin-2 (green), Ser276 phosphorylated RelA (p-RelA, red) and nucleus (blue). E Differences in expression of α-Actinin-2 and p-RelA between HAGC and NAGC, and relationship of overall survival proportion of patients based on univariate analyses. * P < 0.05 using the two-sided <t>Pearson</t> chi-squared tests. F Overall survival proportion of patients with HAGC and NAGC stratified by α-Actinin-2 and p-RelA simultaneous high expression based on Kaplan–Meier survival analyses. HAGC-yes: α-Actinin-2 and p-RelA were simultaneously highly expressed in HAGC sample. HAGC-no: at least one marker in α-Actinin-2 and p-RelA had low expression in HAGC sample. Same to NAGC-yes and NAGC-no. * P < 0.05 using Kaplan–Meier plots and compared with the log-rank test. G and H SUN-16 cells were transfected with or without RelA for 24 h, then the cell nuclear extracts were subjected to ChIP analysis using the p-RelA antibody. G Western blotting analysis with the p-RelA antibody demonstrates the IP specificity and efficiency. H DNA isolated and purified from immunoprecipitated material was amplified with primers spanning the RelA binding site (Fs/Rs) or primers far away from the RelA binding site (Ff/Rf) of the ACTN2 gene promoter
Two Sided Pearson Chi Squared Tests, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/two+sided+chi+square+test/pmc10280853-156-7-8
Average 90 stars, based on 1 article reviews
two-sided pearson chi-squared tests - by Bioz Stars, 2026-09
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chi-squared tests pearson chi-square  (CH Instruments)
90
CH Instruments chi-squared tests pearson chi-square
ACTN2 is a direct target gene of the NF-κB signaling in GC cells. A c-Fos, NFATc1, RelA, Smad3 or Smad4 was cotransfacted with the ACTN2 promoter (-1918/ + 2455) reporter gene ( ACTN2 Luc-R) into SNU-16 cells for 12 h and dual-luciferase reporter assays were performed. B ACTN2 promoter 5′ sequential deletion constructs. Fragments of different lengths of the ACTN2 promoter with the same 3′ end were cloned into pGL3-Basic. RelA was co-transfected with each of the ACTN2 promoter-based reporters for 12 h and dual-luciferase reporter assays were performed. C Mutation of putative RelA binding site at the ACTN2 promoter. SNU-16 cells were transfected with wild type or mutant ACTN2 Luc-R for 12 h and dual-luciferase reporter assays were performed. The statistical analysis information in A-C: The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Ordinary one-way ANOVA. D NAGC and HAGC tissue sections obtained after infusion were immunostained for α-Actinin-2 (green), Ser276 phosphorylated RelA (p-RelA, red) and nucleus (blue). E Differences in expression of α-Actinin-2 and p-RelA between HAGC and NAGC, and relationship of overall survival proportion of patients based on univariate analyses. * P < 0.05 using the two-sided <t>Pearson</t> chi-squared tests. F Overall survival proportion of patients with HAGC and NAGC stratified by α-Actinin-2 and p-RelA simultaneous high expression based on Kaplan–Meier survival analyses. HAGC-yes: α-Actinin-2 and p-RelA were simultaneously highly expressed in HAGC sample. HAGC-no: at least one marker in α-Actinin-2 and p-RelA had low expression in HAGC sample. Same to NAGC-yes and NAGC-no. * P < 0.05 using Kaplan–Meier plots and compared with the log-rank test. G and H SUN-16 cells were transfected with or without RelA for 24 h, then the cell nuclear extracts were subjected to ChIP analysis using the p-RelA antibody. G Western blotting analysis with the p-RelA antibody demonstrates the IP specificity and efficiency. H DNA isolated and purified from immunoprecipitated material was amplified with primers spanning the RelA binding site (Fs/Rs) or primers far away from the RelA binding site (Ff/Rf) of the ACTN2 gene promoter
Chi Squared Tests Pearson Chi Square, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/chi+squared+tests+pearson+chi+square/pmc06043873-73-5-8
Average 90 stars, based on 1 article reviews
chi-squared tests pearson chi-square - by Bioz Stars, 2026-09
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wisc-v  (NCS Pearson Inc)
90
NCS Pearson Inc wisc-v
ACTN2 is a direct target gene of the NF-κB signaling in GC cells. A c-Fos, NFATc1, RelA, Smad3 or Smad4 was cotransfacted with the ACTN2 promoter (-1918/ + 2455) reporter gene ( ACTN2 Luc-R) into SNU-16 cells for 12 h and dual-luciferase reporter assays were performed. B ACTN2 promoter 5′ sequential deletion constructs. Fragments of different lengths of the ACTN2 promoter with the same 3′ end were cloned into pGL3-Basic. RelA was co-transfected with each of the ACTN2 promoter-based reporters for 12 h and dual-luciferase reporter assays were performed. C Mutation of putative RelA binding site at the ACTN2 promoter. SNU-16 cells were transfected with wild type or mutant ACTN2 Luc-R for 12 h and dual-luciferase reporter assays were performed. The statistical analysis information in A-C: The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Ordinary one-way ANOVA. D NAGC and HAGC tissue sections obtained after infusion were immunostained for α-Actinin-2 (green), Ser276 phosphorylated RelA (p-RelA, red) and nucleus (blue). E Differences in expression of α-Actinin-2 and p-RelA between HAGC and NAGC, and relationship of overall survival proportion of patients based on univariate analyses. * P < 0.05 using the two-sided <t>Pearson</t> chi-squared tests. F Overall survival proportion of patients with HAGC and NAGC stratified by α-Actinin-2 and p-RelA simultaneous high expression based on Kaplan–Meier survival analyses. HAGC-yes: α-Actinin-2 and p-RelA were simultaneously highly expressed in HAGC sample. HAGC-no: at least one marker in α-Actinin-2 and p-RelA had low expression in HAGC sample. Same to NAGC-yes and NAGC-no. * P < 0.05 using Kaplan–Meier plots and compared with the log-rank test. G and H SUN-16 cells were transfected with or without RelA for 24 h, then the cell nuclear extracts were subjected to ChIP analysis using the p-RelA antibody. G Western blotting analysis with the p-RelA antibody demonstrates the IP specificity and efficiency. H DNA isolated and purified from immunoprecipitated material was amplified with primers spanning the RelA binding site (Fs/Rs) or primers far away from the RelA binding site (Ff/Rf) of the ACTN2 gene promoter
Wisc V, supplied by NCS Pearson Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/wisc+v/pmc10193466-116-18-3
Average 90 stars, based on 1 article reviews
wisc-v - by Bioz Stars, 2026-09
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pearson chi square evaluation test  (CH Instruments)
90
CH Instruments pearson chi square evaluation test
ACTN2 is a direct target gene of the NF-κB signaling in GC cells. A c-Fos, NFATc1, RelA, Smad3 or Smad4 was cotransfacted with the ACTN2 promoter (-1918/ + 2455) reporter gene ( ACTN2 Luc-R) into SNU-16 cells for 12 h and dual-luciferase reporter assays were performed. B ACTN2 promoter 5′ sequential deletion constructs. Fragments of different lengths of the ACTN2 promoter with the same 3′ end were cloned into pGL3-Basic. RelA was co-transfected with each of the ACTN2 promoter-based reporters for 12 h and dual-luciferase reporter assays were performed. C Mutation of putative RelA binding site at the ACTN2 promoter. SNU-16 cells were transfected with wild type or mutant ACTN2 Luc-R for 12 h and dual-luciferase reporter assays were performed. The statistical analysis information in A-C: The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Ordinary one-way ANOVA. D NAGC and HAGC tissue sections obtained after infusion were immunostained for α-Actinin-2 (green), Ser276 phosphorylated RelA (p-RelA, red) and nucleus (blue). E Differences in expression of α-Actinin-2 and p-RelA between HAGC and NAGC, and relationship of overall survival proportion of patients based on univariate analyses. * P < 0.05 using the two-sided <t>Pearson</t> chi-squared tests. F Overall survival proportion of patients with HAGC and NAGC stratified by α-Actinin-2 and p-RelA simultaneous high expression based on Kaplan–Meier survival analyses. HAGC-yes: α-Actinin-2 and p-RelA were simultaneously highly expressed in HAGC sample. HAGC-no: at least one marker in α-Actinin-2 and p-RelA had low expression in HAGC sample. Same to NAGC-yes and NAGC-no. * P < 0.05 using Kaplan–Meier plots and compared with the log-rank test. G and H SUN-16 cells were transfected with or without RelA for 24 h, then the cell nuclear extracts were subjected to ChIP analysis using the p-RelA antibody. G Western blotting analysis with the p-RelA antibody demonstrates the IP specificity and efficiency. H DNA isolated and purified from immunoprecipitated material was amplified with primers spanning the RelA binding site (Fs/Rs) or primers far away from the RelA binding site (Ff/Rf) of the ACTN2 gene promoter
Pearson Chi Square Evaluation Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/pearson+chi+square+evaluation+test/10__26717_slash_bjstr__2024__54__008572-58-5-6
Average 90 stars, based on 1 article reviews
pearson chi square evaluation test - by Bioz Stars, 2026-09
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w2 and pearson correlation tests  (RStudio)
90
RStudio w2 and pearson correlation tests
ACTN2 is a direct target gene of the NF-κB signaling in GC cells. A c-Fos, NFATc1, RelA, Smad3 or Smad4 was cotransfacted with the ACTN2 promoter (-1918/ + 2455) reporter gene ( ACTN2 Luc-R) into SNU-16 cells for 12 h and dual-luciferase reporter assays were performed. B ACTN2 promoter 5′ sequential deletion constructs. Fragments of different lengths of the ACTN2 promoter with the same 3′ end were cloned into pGL3-Basic. RelA was co-transfected with each of the ACTN2 promoter-based reporters for 12 h and dual-luciferase reporter assays were performed. C Mutation of putative RelA binding site at the ACTN2 promoter. SNU-16 cells were transfected with wild type or mutant ACTN2 Luc-R for 12 h and dual-luciferase reporter assays were performed. The statistical analysis information in A-C: The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Ordinary one-way ANOVA. D NAGC and HAGC tissue sections obtained after infusion were immunostained for α-Actinin-2 (green), Ser276 phosphorylated RelA (p-RelA, red) and nucleus (blue). E Differences in expression of α-Actinin-2 and p-RelA between HAGC and NAGC, and relationship of overall survival proportion of patients based on univariate analyses. * P < 0.05 using the two-sided <t>Pearson</t> chi-squared tests. F Overall survival proportion of patients with HAGC and NAGC stratified by α-Actinin-2 and p-RelA simultaneous high expression based on Kaplan–Meier survival analyses. HAGC-yes: α-Actinin-2 and p-RelA were simultaneously highly expressed in HAGC sample. HAGC-no: at least one marker in α-Actinin-2 and p-RelA had low expression in HAGC sample. Same to NAGC-yes and NAGC-no. * P < 0.05 using Kaplan–Meier plots and compared with the log-rank test. G and H SUN-16 cells were transfected with or without RelA for 24 h, then the cell nuclear extracts were subjected to ChIP analysis using the p-RelA antibody. G Western blotting analysis with the p-RelA antibody demonstrates the IP specificity and efficiency. H DNA isolated and purified from immunoprecipitated material was amplified with primers spanning the RelA binding site (Fs/Rs) or primers far away from the RelA binding site (Ff/Rf) of the ACTN2 gene promoter
W2 And Pearson Correlation Tests, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/w2+and+pearson+correlation+tests/pm32293908-21-7-11
Average 90 stars, based on 1 article reviews
w2 and pearson correlation tests - by Bioz Stars, 2026-09
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w2 pearson test (chi-square)  (CH Instruments)
90
CH Instruments w2 pearson test (chi-square)
ACTN2 is a direct target gene of the NF-κB signaling in GC cells. A c-Fos, NFATc1, RelA, Smad3 or Smad4 was cotransfacted with the ACTN2 promoter (-1918/ + 2455) reporter gene ( ACTN2 Luc-R) into SNU-16 cells for 12 h and dual-luciferase reporter assays were performed. B ACTN2 promoter 5′ sequential deletion constructs. Fragments of different lengths of the ACTN2 promoter with the same 3′ end were cloned into pGL3-Basic. RelA was co-transfected with each of the ACTN2 promoter-based reporters for 12 h and dual-luciferase reporter assays were performed. C Mutation of putative RelA binding site at the ACTN2 promoter. SNU-16 cells were transfected with wild type or mutant ACTN2 Luc-R for 12 h and dual-luciferase reporter assays were performed. The statistical analysis information in A-C: The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Ordinary one-way ANOVA. D NAGC and HAGC tissue sections obtained after infusion were immunostained for α-Actinin-2 (green), Ser276 phosphorylated RelA (p-RelA, red) and nucleus (blue). E Differences in expression of α-Actinin-2 and p-RelA between HAGC and NAGC, and relationship of overall survival proportion of patients based on univariate analyses. * P < 0.05 using the two-sided <t>Pearson</t> chi-squared tests. F Overall survival proportion of patients with HAGC and NAGC stratified by α-Actinin-2 and p-RelA simultaneous high expression based on Kaplan–Meier survival analyses. HAGC-yes: α-Actinin-2 and p-RelA were simultaneously highly expressed in HAGC sample. HAGC-no: at least one marker in α-Actinin-2 and p-RelA had low expression in HAGC sample. Same to NAGC-yes and NAGC-no. * P < 0.05 using Kaplan–Meier plots and compared with the log-rank test. G and H SUN-16 cells were transfected with or without RelA for 24 h, then the cell nuclear extracts were subjected to ChIP analysis using the p-RelA antibody. G Western blotting analysis with the p-RelA antibody demonstrates the IP specificity and efficiency. H DNA isolated and purified from immunoprecipitated material was amplified with primers spanning the RelA binding site (Fs/Rs) or primers far away from the RelA binding site (Ff/Rf) of the ACTN2 gene promoter
W2 Pearson Test (Chi Square), supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pearson+t+test/w2+pearson+test++chi+square+/pm16988993-134-1-4
Average 90 stars, based on 1 article reviews
w2 pearson test (chi-square) - by Bioz Stars, 2026-09
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Image Search Results


Univariate analysis of C2 E318D genotype with in-hospital mortality. : Individuals with the GA genotype appeared to have a higher mortality rate than the GG genotype category. The p-value for this using Pearson's chi-square test was 0.05. The AA genotype was excluded from the analysis because only a single individual had this genotype

Journal:

Article Title: Genetic Variation in Complement Component 2 of the Classical Complement Pathway is Associated with Increased Mortality and Infection: A Study of 627 Trauma Patients

doi: 10.1097/TA.0b013e31819ea61a

Figure Lengend Snippet: Univariate analysis of C2 E318D genotype with in-hospital mortality. : Individuals with the GA genotype appeared to have a higher mortality rate than the GG genotype category. The p-value for this using Pearson's chi-square test was 0.05. The AA genotype was excluded from the analysis because only a single individual had this genotype

Article Snippet: C2 E318D single nucleotide polymorphism (SNP) genotype frequencies and other categorical variables were compared between outcome categories using the Pearson chi-squared test or the Fisher exact test.

Techniques:

Univariate analysis of C2 E318D genotype with Ventilator-Associated Pneumonia (VAP) Individuals with the GA genotype appeared to have a higher VAP rate than the GG genotype category. The p-value for this using Pearson's chi-square test was 0.06. The AA genotype was excluded from the analysis because only a single individual had this genotype.

Journal:

Article Title: Genetic Variation in Complement Component 2 of the Classical Complement Pathway is Associated with Increased Mortality and Infection: A Study of 627 Trauma Patients

doi: 10.1097/TA.0b013e31819ea61a

Figure Lengend Snippet: Univariate analysis of C2 E318D genotype with Ventilator-Associated Pneumonia (VAP) Individuals with the GA genotype appeared to have a higher VAP rate than the GG genotype category. The p-value for this using Pearson's chi-square test was 0.06. The AA genotype was excluded from the analysis because only a single individual had this genotype.

Article Snippet: C2 E318D single nucleotide polymorphism (SNP) genotype frequencies and other categorical variables were compared between outcome categories using the Pearson chi-squared test or the Fisher exact test.

Techniques:

ACTN2 is a direct target gene of the NF-κB signaling in GC cells. A c-Fos, NFATc1, RelA, Smad3 or Smad4 was cotransfacted with the ACTN2 promoter (-1918/ + 2455) reporter gene ( ACTN2 Luc-R) into SNU-16 cells for 12 h and dual-luciferase reporter assays were performed. B ACTN2 promoter 5′ sequential deletion constructs. Fragments of different lengths of the ACTN2 promoter with the same 3′ end were cloned into pGL3-Basic. RelA was co-transfected with each of the ACTN2 promoter-based reporters for 12 h and dual-luciferase reporter assays were performed. C Mutation of putative RelA binding site at the ACTN2 promoter. SNU-16 cells were transfected with wild type or mutant ACTN2 Luc-R for 12 h and dual-luciferase reporter assays were performed. The statistical analysis information in A-C: The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Ordinary one-way ANOVA. D NAGC and HAGC tissue sections obtained after infusion were immunostained for α-Actinin-2 (green), Ser276 phosphorylated RelA (p-RelA, red) and nucleus (blue). E Differences in expression of α-Actinin-2 and p-RelA between HAGC and NAGC, and relationship of overall survival proportion of patients based on univariate analyses. * P < 0.05 using the two-sided Pearson chi-squared tests. F Overall survival proportion of patients with HAGC and NAGC stratified by α-Actinin-2 and p-RelA simultaneous high expression based on Kaplan–Meier survival analyses. HAGC-yes: α-Actinin-2 and p-RelA were simultaneously highly expressed in HAGC sample. HAGC-no: at least one marker in α-Actinin-2 and p-RelA had low expression in HAGC sample. Same to NAGC-yes and NAGC-no. * P < 0.05 using Kaplan–Meier plots and compared with the log-rank test. G and H SUN-16 cells were transfected with or without RelA for 24 h, then the cell nuclear extracts were subjected to ChIP analysis using the p-RelA antibody. G Western blotting analysis with the p-RelA antibody demonstrates the IP specificity and efficiency. H DNA isolated and purified from immunoprecipitated material was amplified with primers spanning the RelA binding site (Fs/Rs) or primers far away from the RelA binding site (Ff/Rf) of the ACTN2 gene promoter

Journal: Journal of Translational Medicine

Article Title: Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer

doi: 10.1186/s12967-023-04156-w

Figure Lengend Snippet: ACTN2 is a direct target gene of the NF-κB signaling in GC cells. A c-Fos, NFATc1, RelA, Smad3 or Smad4 was cotransfacted with the ACTN2 promoter (-1918/ + 2455) reporter gene ( ACTN2 Luc-R) into SNU-16 cells for 12 h and dual-luciferase reporter assays were performed. B ACTN2 promoter 5′ sequential deletion constructs. Fragments of different lengths of the ACTN2 promoter with the same 3′ end were cloned into pGL3-Basic. RelA was co-transfected with each of the ACTN2 promoter-based reporters for 12 h and dual-luciferase reporter assays were performed. C Mutation of putative RelA binding site at the ACTN2 promoter. SNU-16 cells were transfected with wild type or mutant ACTN2 Luc-R for 12 h and dual-luciferase reporter assays were performed. The statistical analysis information in A-C: The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Ordinary one-way ANOVA. D NAGC and HAGC tissue sections obtained after infusion were immunostained for α-Actinin-2 (green), Ser276 phosphorylated RelA (p-RelA, red) and nucleus (blue). E Differences in expression of α-Actinin-2 and p-RelA between HAGC and NAGC, and relationship of overall survival proportion of patients based on univariate analyses. * P < 0.05 using the two-sided Pearson chi-squared tests. F Overall survival proportion of patients with HAGC and NAGC stratified by α-Actinin-2 and p-RelA simultaneous high expression based on Kaplan–Meier survival analyses. HAGC-yes: α-Actinin-2 and p-RelA were simultaneously highly expressed in HAGC sample. HAGC-no: at least one marker in α-Actinin-2 and p-RelA had low expression in HAGC sample. Same to NAGC-yes and NAGC-no. * P < 0.05 using Kaplan–Meier plots and compared with the log-rank test. G and H SUN-16 cells were transfected with or without RelA for 24 h, then the cell nuclear extracts were subjected to ChIP analysis using the p-RelA antibody. G Western blotting analysis with the p-RelA antibody demonstrates the IP specificity and efficiency. H DNA isolated and purified from immunoprecipitated material was amplified with primers spanning the RelA binding site (Fs/Rs) or primers far away from the RelA binding site (Ff/Rf) of the ACTN2 gene promoter

Article Snippet: * P < 0.05 using the two-sided Pearson chi-squared tests.

Techniques: Luciferase, Construct, Clone Assay, Transfection, Mutagenesis, Binding Assay, Expressing, Marker, Western Blot, Isolation, Purification, Immunoprecipitation, Amplification