Journal: Cancer Gene Therapy

Article Title: Cell-intrinsic platinum response and associated genetic and gene expression signatures in ovarian cancer

doi: 10.1038/s41417-025-00941-5

Figure Lengend Snippet: A Wound healing assay for the PEA1/PEA2 ( N = 3 independent replicates), OVCAR3 ( N = 4), OVCAR4 ( N = 4), and PEO1/4 ( N = 3) isogenic cell line pairs. Error bars represent standard deviation. * indicates p -value < 0.05 in paired t-tests and ns indicates non-significant p -value > 0.05. B Representative ALDH1A1 western blot images of all isogenic cell line pairs along with platinum resistant cell lines JHOS2 and COV362. GAPDH serves as a loading control. C CD133 and ALDH1A1 western blot images of all isogenic cell line pairs with or without cisplatin treatment at each respective cell line’s IC 50 for 72 hours. GAPDH serves as a loading control. D Volcano plot showing differential expression between isogenic platinum-resistant cell lines, comparing resistant to their sensitive counterpart. Points are color-coded based on the specific pair, and statistically significant ( | log2 fold change |>1.5, adjusted p -value < 0.05) genes are labeled by gene name.

Article Snippet: PEA1 (ECACC), PEA2 (ECACC), PEO1 (CancerTools.org), PEO4 (ECACC), PEO6 (ECACC) were cultured in RPMI 1640 with 10% FBS and 1 mM NaP.

Techniques: Wound Healing Assay, Standard Deviation, Western Blot, Control, Quantitative Proteomics, Labeling

Journal: Oncology Letters

Article Title: Differential and divergent activity of insulin-like growth factor binding protein 6 in platinum-sensitive versus platinum-resistant high-grade serous ovarian carcinoma cell lines

doi: 10.3892/ol.2022.13305

Figure Lengend Snippet: IGFBP6 expression levels and ERK pathway analysis in HGSOC PEA1 and PEA2 cell lines. IGFBP6 (A) quantitative PCR and (B) immunoblot analysis in PEA1 and PEA2 OC cells lines. β-actin was used as loading marker. *P<0.05. (C) p-ERK1/2 immunoblot analysis in PEA1 and PEA2 cells cultured in serum-free medium overnight and subsequently stimulated with IGFBP6 recombinant protein (1 µg/ml) for 10 min. GAPDH was used as loading marker. (B and C) Histograms represent band intensity ± SD and the ratio of phosphorylated/total proteins from the densitometric analysis of three independent experiments. *P≤0.05. IGFBP6, insulin-like growth factor binding protein 6; ERK, extracellular signal-regulated kinase; HGSOC, high-grade serous ovarian carcinoma; PEA1, pleural effusion adenocarcinoma-1; PEA2, pleural effusion adenocarcinoma-2; PCR, polymerase chain reaction; OC, ovarian cancer; p-ERK1/2, phosphorylated-extracellular signal-regulated kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were purchased from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Marker, Cell Culture, Recombinant, Binding Assay, Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Differential and divergent activity of insulin-like growth factor binding protein 6 in platinum-sensitive versus platinum-resistant high-grade serous ovarian carcinoma cell lines

doi: 10.3892/ol.2022.13305

Figure Lengend Snippet: Hallmarks significantly enriched in PEA1 cells stimulated with IGFBP6.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were purchased from European Collection of Authenticated Cell Cultures (ECACC).

Techniques:

Journal: Oncology Letters

Article Title: Differential and divergent activity of insulin-like growth factor binding protein 6 in platinum-sensitive versus platinum-resistant high-grade serous ovarian carcinoma cell lines

doi: 10.3892/ol.2022.13305

Figure Lengend Snippet: Gene expression profiles of OC platinum-sensitive PEA1 and platinum-resistant PEA2 cells stimulated with IGFBP6. Comparative gene expression (microarray vs. quantitative PCR) of genes reported in and in (A) PEA1 and (B) PEA2 cells. P-values respect to unstimulated control: *P<0.05; **P<0.001; ***P<0.0001. (C) Statistical correlation between the expression of IGFBP6 and FOS (R=0.222, P=1.61e-04, T=3.826, degrees of freedom=283) or EGR1 (R=0.232, P=7.69e-05, T=4.013 degrees of freedom=283) based on the Tumor Ovarian-Bowtell dataset and analyzed via R2: Genomics Analysis and Visualization Platform. OC, ovarian cancer; PEA1, pleural effusion adenocarcinoma-1; PEA2, pleural effusion adenocarcinoma-2; IGFBP6, insulin-like growth factor binding protein 6; FOS, proto-oncogene, AP-1 transcription factor subunit; EGR1, early growth response 1.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were purchased from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Gene Expression, Microarray, Real-time Polymerase Chain Reaction, Control, Expressing, Binding Assay

Journal: Oncology Letters

Article Title: Differential and divergent activity of insulin-like growth factor binding protein 6 in platinum-sensitive versus platinum-resistant high-grade serous ovarian carcinoma cell lines

doi: 10.3892/ol.2022.13305

Figure Lengend Snippet: Expression levels of selected genes in PEA1 data set.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were purchased from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Differential and divergent activity of insulin-like growth factor binding protein 6 in platinum-sensitive versus platinum-resistant high-grade serous ovarian carcinoma cell lines

doi: 10.3892/ol.2022.13305

Figure Lengend Snippet: Qualitative comparison of signaling pathways between PEA1 and PEA2 datasets. (A) Number of pathways significantly regulated in PEA1 and PEA2 datasets. (B) Venn diagram reporting the overlap between pathways significantly modulated by IGFBP6 in PEA1 and PEA2 datasets. (C) Bar chart reporting the top ten common pathways between PEA1 and PEA2 datasets referred to normalized enrichment scores. PEA1, pleural effusion adenocarcinoma-1; PEA2, pleural effusion adenocarcinoma-2; IGFBP6, insulin-like growth factor binding protein 6.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were purchased from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Comparison, Protein-Protein interactions, Binding Assay

Journal: Oncology Letters

Article Title: Differential and divergent activity of insulin-like growth factor binding protein 6 in platinum-sensitive versus platinum-resistant high-grade serous ovarian carcinoma cell lines

doi: 10.3892/ol.2022.13305

Figure Lengend Snippet: Analysis of IL6 pathway in PEA1 and PEA2 cells exposed to IGFBP6. IL6 (A) immunoblot and (B) quantitative PCR expression analysis in PEA1 and PEA2 cells stimulated with IGFBP6 recombinant protein (1 µg/ml) for 2 h. (C) Total and phosphorylated STAT3 immunoblot analysis in PEA1 and PEA2 cells stimulated with IGFBP6 recombinant protein (1 µg/ml) for 2 h. β-actin was used as loading marker. (A and C) Histograms represent band intensity ± SD and the ratio of phosphorylated/total proteins from the densitometric analysis of three independent experiments. *P≤0.05; **P≤0.001; ***P≤0.0001. IL6, interleukin 6; PEA1, pleural effusion adenocarcinoma-1; PEA2, pleural effusion adenocarcinoma-2; IGFBP6, insulin-like growth factor binding protein 6; PCR, polymerase chain reaction; STAT3, signal transducer and activator of transcription 3.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were purchased from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Western Blot, Real-time Polymerase Chain Reaction, Expressing, Recombinant, Marker, Binding Assay, Polymerase Chain Reaction

Journal:

Article Title: The conserved metalloprotease invadolysin localizes to the surface of lipid droplets

doi: 10.1242/jcs.044610

Figure Lengend Snippet: Determination of the relative abundance of different human invadolysin splice variants. (A) Map showing the relative location of the Hv2f and Hv2r primer combination and the expected size of PCR products amplified from different N-terminal variants. (B) Time course of RT-PCR reactions using the Hv2f and Hv2r primer combination after the indicated number of cycles (#) in HeLa, A375MM, A375 and PEO14 cells. Variant 1 (V1) is considerably more abundant than variant 2 (V2) based on the relative intensity of the respective 365 bp and 244 bp bands after different cycles of amplification. (C) Map showing the relative location of the H12f and H12r primers flanking exon 12 and the expected size of PCR products amplified from different variants. (D) Time course of RT-PCR reactions using the H12f and H12r primers. The upper 394 bp product band corresponding to variants containing the 37 amino acid region encoded by exon 12 generally appears more abundant than the 283 bp band corresponding to Δ37 variants.

Article Snippet: PEA1 and PEO14 (human ovarian carcinoma) cell lines were kindly provided by Grant Sellar (Wyeth Research, Dundee, UK).

Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Variant Assay