Journal: Cancer Gene Therapy

Article Title: Cell-intrinsic platinum response and associated genetic and gene expression signatures in ovarian cancer

doi: 10.1038/s41417-025-00941-5

Figure Lengend Snippet: A Wound healing assay for the PEA1/PEA2 ( N = 3 independent replicates), OVCAR3 ( N = 4), OVCAR4 ( N = 4), and PEO1/4 ( N = 3) isogenic cell line pairs. Error bars represent standard deviation. * indicates p -value < 0.05 in paired t-tests and ns indicates non-significant p -value > 0.05. B Representative ALDH1A1 western blot images of all isogenic cell line pairs along with platinum resistant cell lines JHOS2 and COV362. GAPDH serves as a loading control. C CD133 and ALDH1A1 western blot images of all isogenic cell line pairs with or without cisplatin treatment at each respective cell line’s IC 50 for 72 hours. GAPDH serves as a loading control. D Volcano plot showing differential expression between isogenic platinum-resistant cell lines, comparing resistant to their sensitive counterpart. Points are color-coded based on the specific pair, and statistically significant ( | log2 fold change |>1.5, adjusted p -value < 0.05) genes are labeled by gene name.

Article Snippet: PEA1 (ECACC), PEA2 (ECACC), PEO1 (CancerTools.org), PEO4 (ECACC), PEO6 (ECACC) were cultured in RPMI 1640 with 10% FBS and 1 mM NaP.

Techniques: Wound Healing Assay, Standard Deviation, Western Blot, Control, Quantitative Proteomics, Labeling

Journal: American Journal of Cancer Research

Article Title: Different mechanisms underlie IL-6 release in chemosensitive and chemoresistant ovarian carcinoma cells

doi:

Figure Lengend Snippet: IL-6 intracellular levels in PEA1 and PEA2 cells. Cells (1×105/mL) were incubated in RPMI 1640 medium at 37°C in a 5% CO2 atmosphere for 6 hrs. (A) Supernatants were collected and analysed for IL-6 content by ELISA. (B) Effect of AKT or JNK inhibitors on IL-6 release by PEA1 and PEA2 cells. Cells (1×105/mL) were incubated in RPMI 1640 medium, in the presence or absence of 4 μM AKT inhibitor (AKT Inhibitor IV, Calbiochem) or 10 μM JNK inhibitor (SP600125, Sigma-Aldrich) at 37°C in a 5% CO2 atmosphere for 6 hrs. Cell lysates were analysed by western blot with an anti-IL-6 (ab6672, Abcam), an anti-pAKT (Ser473) (#9271, Cell signaling), an anti-AKT (#9272, Cell signaling) or an anti-GAPDH (sc-32233, Santa Cruz Biotechnology) antibody. (C) Supernatants from (B) were collected and IL-6 content was analysed by ELISA. All data were obtained from triplicate samples and confirmed in three separate experiments.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: American Journal of Cancer Research

Article Title: Different mechanisms underlie IL-6 release in chemosensitive and chemoresistant ovarian carcinoma cells

doi:

Figure Lengend Snippet: AKT affect IL-6 secretion in PEA1 cells. Cells (1×105/mL) were incubated in RPMI 1640 medium, in the presence or absence of AKT inhibitors at different concentrations for 6 hrs. (A) Cell lysates were analysed by western blot as described in Figure 2. (B) Supernatants were collected and IL-6 content was analysed by ELISA. Data are from triplicate samples and confirmed in two separate experiments. (C) PEA1 and PEA2 cells were transfected with akt siRNAs or a non-targeted (NT) siRNA at a final concentration of 50 nmol/L for 48 hours. Next, the cells were harvested, lysed and analysed by Western blotting with the indicated antibodies. (D) Supernatants from (C) were collected and IL-6 content was analysed by ELISA. Data were obtained from triplicate samples and confirmed in two separate experiments.

Article Snippet: The human ovarian adenocarcinoma cell lines PEA1 and PEA2 were obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay, Transfection, Concentration Assay