Journal: Data in Brief

Article Title: Data on the genome and proteome profiles of ciprofloxacin-resistant Acholeplasma laidlawii strains selected under different conditions in vitro

doi: 10.1016/j.dib.2020.106412

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Article Snippet: Description of data collection , Three ciprofloxacin-resistant Acholeplasma laidlawii strains were selected under different conditions in vitro : sequentially inoculated in a broth medium that contained increasing concentrations of ciprofloxacin, co-culturing of ciprofloxacin-sensitive strain with and without extracellular vesicles derived from the high-level ciprofloxacin-resistant mollicute. The vesicles of A. laidlawii were obtained by ultracentrifugation; their purity was validated by PCR. Genome sequencing was performed on the NextSeq Illumina platform. Search and annotation of single nucleotide polymorphisms were performed using Samtools and SnpEff, respectively. Cellular proteins were separated by 2DE. Search of differential expressed proteins was performed using PDQuest. Proteins were identified by mass spectrometry..

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Mass Spectrometry, Software, In Vitro, Derivative Assay, Sequencing