pdgfbb Search Results


pdgfbb (dimer), human  (Gold Biotechnology Inc)
89
Gold Biotechnology Inc pdgfbb (dimer), human
Pdgfbb (Dimer), Human, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/custom%401150-10%4034831221?v=Gold+Biotechnology+Inc
Average 89 stars, based on 1 article reviews
pdgfbb (dimer), human - by Bioz Stars, 2026-08
89/100 stars
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vegfa  (Bioss)
92
Bioss vegfa
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Vegfa, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/pmc07904259-379-29-35?v=Bioss
Average 92 stars, based on 1 article reviews
vegfa - by Bioz Stars, 2026-08
92/100 stars
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pdgf bb  (Proteintech)
93
Proteintech pdgf bb
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Pdgf Bb, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/pmc08011465-165-12-13?v=Proteintech
Average 93 stars, based on 1 article reviews
pdgf bb - by Bioz Stars, 2026-08
93/100 stars
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aptamer complex formation  (Creative BioMart)
90
Creative BioMart aptamer complex formation
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Aptamer Complex Formation, supplied by Creative BioMart, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/us11208663-758-5-18?v=Creative+BioMart
Average 90 stars, based on 1 article reviews
aptamer complex formation - by Bioz Stars, 2026-08
90/100 stars
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anti-pdgfbb antibody  (Becton Dickinson)
90
Becton Dickinson anti-pdgfbb antibody
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Anti Pdgfbb Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/pmc10324274-104-16-18?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti-pdgfbb antibody - by Bioz Stars, 2026-08
90/100 stars
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pdgfbb promega  (Promega)
90
Promega pdgfbb promega
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Pdgfbb Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/10__12659_slash_msm__910986-53-0-1?v=Promega
Average 90 stars, based on 1 article reviews
pdgfbb promega - by Bioz Stars, 2026-08
90/100 stars
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pdgfbb  (Sino Biological)
96
Sino Biological pdgfbb
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Pdgfbb, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/custom%40pdgfbb%4010%2E1158%2F1078-0432%2Eccr-14-1956?v=Sino+Biological
Average 96 stars, based on 1 article reviews
pdgfbb - by Bioz Stars, 2026-08
96/100 stars
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recombinant human pdgfbb  (GL Biochem)
90
GL Biochem recombinant human pdgfbb
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Recombinant Human Pdgfbb, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/pm25558873-53-31-37?v=GL+Biochem
Average 90 stars, based on 1 article reviews
recombinant human pdgfbb - by Bioz Stars, 2026-08
90/100 stars
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pdgfbb  (Strathmann Biotec AG)
90
Strathmann Biotec AG pdgfbb
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Pdgfbb, supplied by Strathmann Biotec AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/pm20407225-36-34-27?v=Strathmann+Biotec+AG
Average 90 stars, based on 1 article reviews
pdgfbb - by Bioz Stars, 2026-08
90/100 stars
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growth factors pdgfbb  (ZymoGenetics inc)
90
ZymoGenetics inc growth factors pdgfbb
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Growth Factors Pdgfbb, supplied by ZymoGenetics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/pm11060232-92-0-11?v=ZymoGenetics+inc
Average 90 stars, based on 1 article reviews
growth factors pdgfbb - by Bioz Stars, 2026-08
90/100 stars
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rh pdgfbb dpc biermann  (DPC Biermann GmbH)
90
DPC Biermann GmbH rh pdgfbb dpc biermann
( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of <t>VEGFA</t> and PDGF-BB in endothelial cells ( E ) and tumor necrosis <t>factor-α</t> <t>(TNF-α),</t> interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Rh Pdgfbb Dpc Biermann, supplied by DPC Biermann GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdgfbb/pm19950208-96-11-13?v=DPC+Biermann+GmbH
Average 90 stars, based on 1 article reviews
rh pdgfbb dpc biermann - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of VEGFA and PDGF-BB in endothelial cells ( E ) and tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Journal: Science Advances

Article Title: Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs

doi: 10.1126/sciadv.abd6740

Figure Lengend Snippet: ( A ) In vitro uptake of the MSN/miR-21-5p complex by adherent endothelial cells (ECs) and macrophages (MCs). ( B ) In vitro transfection efficiency of miR-21-5p was determined by quantifying the miRNA level using real-time quantitative PCR. ( C ) Representative flow cytometry analysis of CD31 levels in ECs and F4/80 levels in MCs. ( D ) In vitro uptake of the MSN/miR-21-5p complex by ECs and MCs was determined by quantifying the double-positive cells (CD31 or F4/80 and Cy3) using flow cytometric analysis. The protein expression levels of VEGFA and PDGF-BB in endothelial cells ( E ) and tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 in macrophages ( F ) were determined by the real-time quantitative PCR and Western blot analysis. ( G ) The endothelial cells that formed three-dimensional (3D) capillary-like tubular structures were evaluated at indicated times (8 and 16 hours). ( H ) Schematic diagram of the experimental setup. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling. ( I ) Apoptosis-positive cardiomyocytes from these treatment groups were further quantified. ( J ) Protein levels of secreted proangiogenic factors were determined by enzyme-linked immunosorbent assay (ELISA) analysis of cell supernatants from the MSN/miRNA-treated ECs (scale bars, 50 μm). * P < 0.05 and *** P < 0.01. All experiments were carried out in triplicate. n = 5 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Article Snippet: The membranes were blocked with 5% bovine serum albumin and incubated with specific primary antibodies against the following: TNF-α (AF-410-NA, R&D, USA), IL-1β (Novus, AF-401-NA), IL-6 (bs-0782R, Bioss, USA), VEGFA (DF7470, Affinity, USA), PDGF-BB (bs-1316R, Bioss), TLR1 (NB100-56563, Novus), TLR2 (Abcam, ab209217), TLR3 (NBP2-24875, Novus), TLR8 (NBP2-24917, Novus), NFκB (CST8242s, Cell Signaling Technology, USA), p-NFκB (CST3033s), SPRY1 (Abcam, ab111523), P-ERK1/2 (AF1018, R&D), ERK1/2 (AF1576, R&D), P-AKT (AF887, R&D), AKT (MAB2055, R&D), P-FAK (MAB4528, R&D), FAK (AF4467, R&D), P-P38 (CST4511), P38 (CST8690), and GAPDH (ab181602) at a ratio of 1:1000 overnight.

Techniques: In Vitro, Transfection, Real-time Polymerase Chain Reaction, Flow Cytometry, Expressing, Western Blot, TUNEL Assay, End Labeling, Enzyme-linked Immunosorbent Assay

( A ) A heatmap of selected proteins representing strongly altered signaling pathways in three datasets of endothelial cells treated with MSN/miR-NC or MSN/miR-21-5p complexes. ( B ) KEGG pathway analysis of both up- and down-regulated pathways in endothelial cells after MSN/miR-21-5p complex treatment. ( C ) Western blot analysis of changes in SPRY1, P-ERK1/2, P-FAK, P-p38, P-AKT, VEGFA, and PDGF-BB protein content alteration in endothelial cells after treatment with the MSN/miR-21-5p complex. ( D ) The effect of MSN/miR-21-5p or MSN/miR-NC on SPRY1 mRNA levels (left) and SPRY1 protein levels (right) in endothelial cells. ( E ) Schematic diagram illustrating the design of luciferase reporters with the WT SPRY1 3′ untranslated region (WT 3′UTR) or the site-directed mutant SPRY1 3′UTR (3′UTR-Mut). ( F ) The effect of MSN/miR-21-5p on luciferase activity in endothelial cells transfected with either the WT SPRY1 3′UTR reporter (left) or the mutant SPRY1 3′UTR reporter (right). ( G ) Western blot analysis of P-ERK1/2, P-FAK, P-p38, P-AKT, VEGFA, and PDGF-BB protein level alteration in MSN/miR-21-5p complex–treated endothelial cells after overexpressing SPRY1 with the SPRY1 overexpression vector. * P < 0.05 and *** P < 0.01. n = 3 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Journal: Science Advances

Article Title: Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs

doi: 10.1126/sciadv.abd6740

Figure Lengend Snippet: ( A ) A heatmap of selected proteins representing strongly altered signaling pathways in three datasets of endothelial cells treated with MSN/miR-NC or MSN/miR-21-5p complexes. ( B ) KEGG pathway analysis of both up- and down-regulated pathways in endothelial cells after MSN/miR-21-5p complex treatment. ( C ) Western blot analysis of changes in SPRY1, P-ERK1/2, P-FAK, P-p38, P-AKT, VEGFA, and PDGF-BB protein content alteration in endothelial cells after treatment with the MSN/miR-21-5p complex. ( D ) The effect of MSN/miR-21-5p or MSN/miR-NC on SPRY1 mRNA levels (left) and SPRY1 protein levels (right) in endothelial cells. ( E ) Schematic diagram illustrating the design of luciferase reporters with the WT SPRY1 3′ untranslated region (WT 3′UTR) or the site-directed mutant SPRY1 3′UTR (3′UTR-Mut). ( F ) The effect of MSN/miR-21-5p on luciferase activity in endothelial cells transfected with either the WT SPRY1 3′UTR reporter (left) or the mutant SPRY1 3′UTR reporter (right). ( G ) Western blot analysis of P-ERK1/2, P-FAK, P-p38, P-AKT, VEGFA, and PDGF-BB protein level alteration in MSN/miR-21-5p complex–treated endothelial cells after overexpressing SPRY1 with the SPRY1 overexpression vector. * P < 0.05 and *** P < 0.01. n = 3 per group. The data are shown as means ± SD. Photo credit: Yan Li, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Article Snippet: The membranes were blocked with 5% bovine serum albumin and incubated with specific primary antibodies against the following: TNF-α (AF-410-NA, R&D, USA), IL-1β (Novus, AF-401-NA), IL-6 (bs-0782R, Bioss, USA), VEGFA (DF7470, Affinity, USA), PDGF-BB (bs-1316R, Bioss), TLR1 (NB100-56563, Novus), TLR2 (Abcam, ab209217), TLR3 (NBP2-24875, Novus), TLR8 (NBP2-24917, Novus), NFκB (CST8242s, Cell Signaling Technology, USA), p-NFκB (CST3033s), SPRY1 (Abcam, ab111523), P-ERK1/2 (AF1018, R&D), ERK1/2 (AF1576, R&D), P-AKT (AF887, R&D), AKT (MAB2055, R&D), P-FAK (MAB4528, R&D), FAK (AF4467, R&D), P-P38 (CST4511), P38 (CST8690), and GAPDH (ab181602) at a ratio of 1:1000 overnight.

Techniques: Western Blot, Luciferase, Mutagenesis, Activity Assay, Transfection, Over Expression, Plasmid Preparation