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NDI-101150 treatment enhanced activation of primary human B cells. ( A ) Percentage of CD69 + B cells after a dose-response treatment with NDI-101150. ( B ) Percent increase in IgG production relative to DMSO in B cells after a dose-response treatment with NDI-101150. ( C ) Percent increase in B-cell proliferation on stimulation with anti-IgM and anti-CD40 after a dose-response treatment with NDI-101150. All data are graphed as the mean±SD from three human donors. ( D ) Schematic representation of the in vivo antigen-specific antibody generation study. On day 0, mice were immunized intravenously with PBS control or with antigen (Ag)—keyhole limpet hemocyanin (KLH), or dinitrophenyl (DNP)-Ficoll—followed by daily treatment with NDI-101150 from days 0 to 14. On days 7 and 14, blood was collected from mice, plasma was isolated, and Ag-specific IgM and IgG responses were determined by <t>ELISA.</t> ( E ) Quantification of KLH-specific IgM and IgG isotypes in blood from mice. ( F ) Quantification of DNP-specific IgM and IgG isotypes in mice. Values are mean±SD n=10 mice per treatment group. Significance was determined by one-way analysis of variance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. QD, once daily; BIW, biweekly; DMSO, dimethylsulfoxide; ip, intraperitoneally; PBS, phosphate-buffered saline.
Mouse Duoset Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems recombinant rat pdgf ab heterodimer
NDI-101150 treatment enhanced activation of primary human B cells. ( A ) Percentage of CD69 + B cells after a dose-response treatment with NDI-101150. ( B ) Percent increase in IgG production relative to DMSO in B cells after a dose-response treatment with NDI-101150. ( C ) Percent increase in B-cell proliferation on stimulation with anti-IgM and anti-CD40 after a dose-response treatment with NDI-101150. All data are graphed as the mean±SD from three human donors. ( D ) Schematic representation of the in vivo antigen-specific antibody generation study. On day 0, mice were immunized intravenously with PBS control or with antigen (Ag)—keyhole limpet hemocyanin (KLH), or dinitrophenyl (DNP)-Ficoll—followed by daily treatment with NDI-101150 from days 0 to 14. On days 7 and 14, blood was collected from mice, plasma was isolated, and Ag-specific IgM and IgG responses were determined by <t>ELISA.</t> ( E ) Quantification of KLH-specific IgM and IgG isotypes in blood from mice. ( F ) Quantification of DNP-specific IgM and IgG isotypes in mice. Values are mean±SD n=10 mice per treatment group. Significance was determined by one-way analysis of variance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. QD, once daily; BIW, biweekly; DMSO, dimethylsulfoxide; ip, intraperitoneally; PBS, phosphate-buffered saline.
Recombinant Rat Pdgf Ab Heterodimer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems quantikine elisa kits
NDI-101150 treatment enhanced activation of primary human B cells. ( A ) Percentage of CD69 + B cells after a dose-response treatment with NDI-101150. ( B ) Percent increase in IgG production relative to DMSO in B cells after a dose-response treatment with NDI-101150. ( C ) Percent increase in B-cell proliferation on stimulation with anti-IgM and anti-CD40 after a dose-response treatment with NDI-101150. All data are graphed as the mean±SD from three human donors. ( D ) Schematic representation of the in vivo antigen-specific antibody generation study. On day 0, mice were immunized intravenously with PBS control or with antigen (Ag)—keyhole limpet hemocyanin (KLH), or dinitrophenyl (DNP)-Ficoll—followed by daily treatment with NDI-101150 from days 0 to 14. On days 7 and 14, blood was collected from mice, plasma was isolated, and Ag-specific IgM and IgG responses were determined by <t>ELISA.</t> ( E ) Quantification of KLH-specific IgM and IgG isotypes in blood from mice. ( F ) Quantification of DNP-specific IgM and IgG isotypes in mice. Values are mean±SD n=10 mice per treatment group. Significance was determined by one-way analysis of variance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. QD, once daily; BIW, biweekly; DMSO, dimethylsulfoxide; ip, intraperitoneally; PBS, phosphate-buffered saline.
Quantikine Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems human pdgf ab
NDI-101150 treatment enhanced activation of primary human B cells. ( A ) Percentage of CD69 + B cells after a dose-response treatment with NDI-101150. ( B ) Percent increase in IgG production relative to DMSO in B cells after a dose-response treatment with NDI-101150. ( C ) Percent increase in B-cell proliferation on stimulation with anti-IgM and anti-CD40 after a dose-response treatment with NDI-101150. All data are graphed as the mean±SD from three human donors. ( D ) Schematic representation of the in vivo antigen-specific antibody generation study. On day 0, mice were immunized intravenously with PBS control or with antigen (Ag)—keyhole limpet hemocyanin (KLH), or dinitrophenyl (DNP)-Ficoll—followed by daily treatment with NDI-101150 from days 0 to 14. On days 7 and 14, blood was collected from mice, plasma was isolated, and Ag-specific IgM and IgG responses were determined by <t>ELISA.</t> ( E ) Quantification of KLH-specific IgM and IgG isotypes in blood from mice. ( F ) Quantification of DNP-specific IgM and IgG isotypes in mice. Values are mean±SD n=10 mice per treatment group. Significance was determined by one-way analysis of variance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. QD, once daily; BIW, biweekly; DMSO, dimethylsulfoxide; ip, intraperitoneally; PBS, phosphate-buffered saline.
Human Pdgf Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems quantikine mouse rat pdgf ab
NDI-101150 treatment enhanced activation of primary human B cells. ( A ) Percentage of CD69 + B cells after a dose-response treatment with NDI-101150. ( B ) Percent increase in IgG production relative to DMSO in B cells after a dose-response treatment with NDI-101150. ( C ) Percent increase in B-cell proliferation on stimulation with anti-IgM and anti-CD40 after a dose-response treatment with NDI-101150. All data are graphed as the mean±SD from three human donors. ( D ) Schematic representation of the in vivo antigen-specific antibody generation study. On day 0, mice were immunized intravenously with PBS control or with antigen (Ag)—keyhole limpet hemocyanin (KLH), or dinitrophenyl (DNP)-Ficoll—followed by daily treatment with NDI-101150 from days 0 to 14. On days 7 and 14, blood was collected from mice, plasma was isolated, and Ag-specific IgM and IgG responses were determined by <t>ELISA.</t> ( E ) Quantification of KLH-specific IgM and IgG isotypes in blood from mice. ( F ) Quantification of DNP-specific IgM and IgG isotypes in mice. Values are mean±SD n=10 mice per treatment group. Significance was determined by one-way analysis of variance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. QD, once daily; BIW, biweekly; DMSO, dimethylsulfoxide; ip, intraperitoneally; PBS, phosphate-buffered saline.
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NDI-101150 treatment enhanced activation of primary human B cells. ( A ) Percentage of CD69 + B cells after a dose-response treatment with NDI-101150. ( B ) Percent increase in IgG production relative to DMSO in B cells after a dose-response treatment with NDI-101150. ( C ) Percent increase in B-cell proliferation on stimulation with anti-IgM and anti-CD40 after a dose-response treatment with NDI-101150. All data are graphed as the mean±SD from three human donors. ( D ) Schematic representation of the in vivo antigen-specific antibody generation study. On day 0, mice were immunized intravenously with PBS control or with antigen (Ag)—keyhole limpet hemocyanin (KLH), or dinitrophenyl (DNP)-Ficoll—followed by daily treatment with NDI-101150 from days 0 to 14. On days 7 and 14, blood was collected from mice, plasma was isolated, and Ag-specific IgM and IgG responses were determined by ELISA. ( E ) Quantification of KLH-specific IgM and IgG isotypes in blood from mice. ( F ) Quantification of DNP-specific IgM and IgG isotypes in mice. Values are mean±SD n=10 mice per treatment group. Significance was determined by one-way analysis of variance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. QD, once daily; BIW, biweekly; DMSO, dimethylsulfoxide; ip, intraperitoneally; PBS, phosphate-buffered saline.

Journal: Journal for Immunotherapy of Cancer

Article Title: Highly selective HPK1 inhibitor NDI-101150 mediates immune cell activation and robust antitumor responses, distinct from immune checkpoint blockade

doi: 10.1136/jitc-2025-012064

Figure Lengend Snippet: NDI-101150 treatment enhanced activation of primary human B cells. ( A ) Percentage of CD69 + B cells after a dose-response treatment with NDI-101150. ( B ) Percent increase in IgG production relative to DMSO in B cells after a dose-response treatment with NDI-101150. ( C ) Percent increase in B-cell proliferation on stimulation with anti-IgM and anti-CD40 after a dose-response treatment with NDI-101150. All data are graphed as the mean±SD from three human donors. ( D ) Schematic representation of the in vivo antigen-specific antibody generation study. On day 0, mice were immunized intravenously with PBS control or with antigen (Ag)—keyhole limpet hemocyanin (KLH), or dinitrophenyl (DNP)-Ficoll—followed by daily treatment with NDI-101150 from days 0 to 14. On days 7 and 14, blood was collected from mice, plasma was isolated, and Ag-specific IgM and IgG responses were determined by ELISA. ( E ) Quantification of KLH-specific IgM and IgG isotypes in blood from mice. ( F ) Quantification of DNP-specific IgM and IgG isotypes in mice. Values are mean±SD n=10 mice per treatment group. Significance was determined by one-way analysis of variance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. QD, once daily; BIW, biweekly; DMSO, dimethylsulfoxide; ip, intraperitoneally; PBS, phosphate-buffered saline.

Article Snippet: Serum cytokines and circulating antibody titers were measured by MSD using mouse Duoset ELISA kits (R&D Systems) according to manufacturer’s instructions.

Techniques: Activation Assay, In Vivo, Control, Clinical Proteomics, Isolation, Enzyme-linked Immunosorbent Assay, Saline