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Image Search Results
Journal: Journal for Immunotherapy of Cancer
Article Title: Highly selective HPK1 inhibitor NDI-101150 mediates immune cell activation and robust antitumor responses, distinct from immune checkpoint blockade
doi: 10.1136/jitc-2025-012064
Figure Lengend Snippet: NDI-101150 treatment enhanced activation of primary human B cells. ( A ) Percentage of CD69 + B cells after a dose-response treatment with NDI-101150. ( B ) Percent increase in IgG production relative to DMSO in B cells after a dose-response treatment with NDI-101150. ( C ) Percent increase in B-cell proliferation on stimulation with anti-IgM and anti-CD40 after a dose-response treatment with NDI-101150. All data are graphed as the mean±SD from three human donors. ( D ) Schematic representation of the in vivo antigen-specific antibody generation study. On day 0, mice were immunized intravenously with PBS control or with antigen (Ag)—keyhole limpet hemocyanin (KLH), or dinitrophenyl (DNP)-Ficoll—followed by daily treatment with NDI-101150 from days 0 to 14. On days 7 and 14, blood was collected from mice, plasma was isolated, and Ag-specific IgM and IgG responses were determined by ELISA. ( E ) Quantification of KLH-specific IgM and IgG isotypes in blood from mice. ( F ) Quantification of DNP-specific IgM and IgG isotypes in mice. Values are mean±SD n=10 mice per treatment group. Significance was determined by one-way analysis of variance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. QD, once daily; BIW, biweekly; DMSO, dimethylsulfoxide; ip, intraperitoneally; PBS, phosphate-buffered saline.
Article Snippet: Serum cytokines and circulating antibody titers were measured by MSD using
Techniques: Activation Assay, In Vivo, Control, Clinical Proteomics, Isolation, Enzyme-linked Immunosorbent Assay, Saline