Journal: iScience

Article Title: Macrophage AMPK β1 activation by PF-06409577 reduces the inflammatory response, cholesterol synthesis, and atherosclerosis in mice.

doi: 10.1016/j.isci.2023.108269

Figure Lengend Snippet: Figure 2. PF-06409577 reduces atherosclerosis via macrophage AMPK activation (A–D) Peritoneal macrophages were isolated from AMPK b1fl/fland AMPK b1LysM mice. (A) AMPK levels and phosphorylation of ACC following treatment with PF- 06409577 (10 mM, 90 min) were detected by western blot and (B–D) quantified using ImageJ data are presented as mean G SEM, * indicates p < 0.05 and *** indicates <0.005 by unpaired t test. AMPK b1fl/fland AMPK b1 LysM mice were injected with PCSK9 AAV, fed a Western diet and treated with Vehicle or 100 mg/kg PF-06409577 by oral gavage for 6 weeks. (E and F) Representative plaque images, scale bar represents 100 mM and quantification. Data are presented as mean G SEM, # indicates p < 0.05 in overall group effect my two-way ANOVA, * indicates p < 0.05 by two-way ANOVA with Fisher’s LSD post-hoc testing.

Article Snippet: Serum PCSK9 levels were analysed using a murine PCSK9 ELISA (Cat# MPC900, R&D Systems) as per the manufacturer’s instructions.

Techniques: Activation Assay, Isolation, Phospho-proteomics, Western Blot, Injection

Journal: Frontiers in Pharmacology

Article Title: Di’ao Xinxuekang Capsule Improves the Anti-Atherosclerotic Effect of Atorvastatin by Downregulating the SREBP2/PCSK9 Signalling Pathway

doi: 10.3389/fphar.2022.857092

Figure Lengend Snippet: The primer sequences for RT-PCR assay.

Article Snippet: The mouse PCSK9 ELISA kit (batch number: E-EL-M0634c) was purchased from Elabscience Biotechnology (Wuhan, China).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Di’ao Xinxuekang Capsule Improves the Anti-Atherosclerotic Effect of Atorvastatin by Downregulating the SREBP2/PCSK9 Signalling Pathway

doi: 10.3389/fphar.2022.857092

Figure Lengend Snippet: Effect of DXXK, ATO, and their combination on serum lipid parameters and serum PCSK9.

Article Snippet: The mouse PCSK9 ELISA kit (batch number: E-EL-M0634c) was purchased from Elabscience Biotechnology (Wuhan, China).

Techniques: Control

Journal: Frontiers in Pharmacology

Article Title: Di’ao Xinxuekang Capsule Improves the Anti-Atherosclerotic Effect of Atorvastatin by Downregulating the SREBP2/PCSK9 Signalling Pathway

doi: 10.3389/fphar.2022.857092

Figure Lengend Snippet: Effect of DXXK, ATO, and their combination on the levels of liver (A) LDLR mRNA and (B) PCSK9 mRNA, (C) LDLR and (D) PCSK9 protein expression in the liver ( n = 5); (E) the representative blot bands. Results are presented as the mean ± SD. ∗ p < 0.05 vs. ApoE −/− model group, ∗∗ p < 0.01 vs. ApoE −/− model group, ∆∆ p < 0.01 vs. ATO (1.3 mg/kg/day.) group.

Article Snippet: The mouse PCSK9 ELISA kit (batch number: E-EL-M0634c) was purchased from Elabscience Biotechnology (Wuhan, China).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Di’ao Xinxuekang Capsule Improves the Anti-Atherosclerotic Effect of Atorvastatin by Downregulating the SREBP2/PCSK9 Signalling Pathway

doi: 10.3389/fphar.2022.857092

Figure Lengend Snippet: The DXXK Improves the Anti‐atherosclerotic Effect of ATO by SREBP2/PCSK9 pathway.

Article Snippet: The mouse PCSK9 ELISA kit (batch number: E-EL-M0634c) was purchased from Elabscience Biotechnology (Wuhan, China).

Techniques:

Journal: Molecular Biology Reports

Article Title: HepG2 cells stimulated by THP-1-conditioned medium: a potential in vitro model of systemic inflammation–induced hepatic alterations

doi: 10.1007/s11033-026-11641-0

Figure Lengend Snippet: Time-dependent changes in the relative mRNA expression of the inflammatory markers NF-κB p50/p105, TNF-α, A20, and SAA ( a ), transcription factors PPARα and LRH-1 ( b ), and lipid metabolism–associated genes LDLr, PON1, PCSK9, ApoA1, ABCA1, and ApoC3 ( c ) in HepG2 cells stimulated with CM (diluted 1:3) derived from THP-1 cells activated with PMA + LPS. Time-course experiments were performed in three independent biological experiments with duplicates for most time points. For selected key time points (4 h and 24 h), which were identified as the most relevant based on previous experiments, an additional experiment with biological quadruplets was conducted to increase precision. Protein expression of ApoA1 and PCSK9 ( d ) in HepG2 cells 24 h after stimulation with CM. Data represent nine biological replicates from three independent experiments. * p < 0,05 ** p < 0,01 *** p < 0,001 **** p < 0,0001

Article Snippet: Western blot was performed using standard protocol with primary antibodies against β-actin (Abcam, Cambridge, UK, EPR21241 ), ApoA1 (Abcam, Cambridge, UK, EP1368Y) and PCSK9 (Cell Signalling Technology, Netherlands, 85813T), and secondary antibodies conjugated to horseradish peroxidase.

Techniques: Expressing, Derivative Assay

Journal: Molecular Biology Reports

Article Title: HepG2 cells stimulated by THP-1-conditioned medium: a potential in vitro model of systemic inflammation–induced hepatic alterations

doi: 10.1007/s11033-026-11641-0

Figure Lengend Snippet: Relative mRNA expression of inflammatory markers, transcription factors and lipid metabolism–associated genes in HepG2 cells after stimulation with CM derived from THP-1 cells treated with PMA + LPS (CM) or from THP-1 cells that were left non-stimulated (NS). mRNA was isolated at suitable time points based on previous experiments: 4 h for NF-κB p50/p105, TNF-α, A20, SAA, PPARα, LRH-1, and LDLr ( a ) and 24 h for PON1, PCSK9, ApoA1, ABCA1, and ApoC3 ( b ). qPCR analyses represent the average of three independent biological experiments. In the first experiment, three biological replicates were analysed. The second and third experiments each involved four biological replicates.* p < 0,05 ** p < 0,01 *** p < 0,001 **** p < 0,0001

Article Snippet: Western blot was performed using standard protocol with primary antibodies against β-actin (Abcam, Cambridge, UK, EPR21241 ), ApoA1 (Abcam, Cambridge, UK, EP1368Y) and PCSK9 (Cell Signalling Technology, Netherlands, 85813T), and secondary antibodies conjugated to horseradish peroxidase.

Techniques: Expressing, Derivative Assay, Isolation