pcr reaction buffer Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Thermo Fisher
    Average 99 stars, based on 29922 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Millipore polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Polymerase Chain Reaction Pcr Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    89
    Roche polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Polymerase Chain Reaction Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/Roche
    Average 89 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    90
    PerkinElmer polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Polymerase Chain Reaction Pcr Buffer, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/PerkinElmer
    Average 90 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    89
    Promega polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Polymerase Chain Reaction Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/Promega
    Average 89 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    89
    GE Healthcare polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Polymerase Chain Reaction Pcr Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/GE Healthcare
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    99
    Thermo Fisher accuprime polymerase chain reaction buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Accuprime Polymerase Chain Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accuprime polymerase chain reaction buffer/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    accuprime polymerase chain reaction buffer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Roche 1x polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    1x Polymerase Chain Reaction Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x polymerase chain reaction pcr buffer/product/Roche
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    1x polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    89
    TaKaRa polymerase chain reaction pcr buffer
    Ligase inhibitor treatment blocked cccDNA formation in nuclear extract. (A) Demonstration of the in vitro cccDNA formation assay. The indicated amount of purified DHBV rcDNA were incubated with HepG2 nuclear extract as described in Materials and Methods. After the in vitro cccDNA formation reaction, total <t>DNA</t> were extracted and subjected to DHBV Southern blot analysis (top panel) and cccDNA-specific <t>PCR</t> assay (bottom panel). The EcoRI-linearized DHBV unit-length DNA was used as marker in Southern blot and as PCR positive template in cccDNA PCR assay (lane 2). 1 ng of rcDNA alone (lane 3) and nuclear extract alone (lane 8) served as PCR negative controls. (B) The in vitro DHBV cccDNA formation reaction was treated with DNA ligases inhibitor L1 (20 μM), L25 (20 μM), or L189 (50 μM), or DMSO solvent, and cccDNA was detected by PCR (lanes 3–6). DHBV cccDNA purified from Dstet5 cells was used as PCR positive control (lane 1). 1 ng of rcDNA without incubation with nuclear extract (lane 7) or without PCR reaction (lane 8) served as negative controls.
    Polymerase Chain Reaction Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/TaKaRa
    Average 89 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    89
    Bioneer Corporation polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/Bioneer Corporation
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    88
    Thermo Fisher polymerase chain reaction conditions 1x pcr gold buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Conditions 1x Pcr Gold Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction conditions 1x pcr gold buffer/product/Thermo Fisher
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction conditions 1x pcr gold buffer - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    Avantor polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/Avantor
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim pcr buffer
    Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated <t>RNA</t> samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) <t>PCR</t> confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.
    Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Boehringer Mannheim
    Average 92 stars, based on 959 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    94
    Thermo Fisher polymerase chain reaction pcr buffer ii
    Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated <t>RNA</t> samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) <t>PCR</t> confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.
    Polymerase Chain Reaction Pcr Buffer Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer ii/product/Thermo Fisher
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer ii - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    80
    PerkinElmer 1x polymerase chain reaction pcr buffer ii
    Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated <t>RNA</t> samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) <t>PCR</t> confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.
    1x Polymerase Chain Reaction Pcr Buffer Ii, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x polymerase chain reaction pcr buffer ii/product/PerkinElmer
    Average 80 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    1x polymerase chain reaction pcr buffer ii - by Bioz Stars, 2020-07
    80/100 stars
      Buy from Supplier

    91
    SolGent polymerase chain reaction pcr buffer
    Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated <t>RNA</t> samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) <t>PCR</t> confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.
    Polymerase Chain Reaction Pcr Buffer, supplied by SolGent, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/SolGent
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    94
    Thermo Fisher polymerase chain reaction pcr taq polymerase
    Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated <t>RNA</t> samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) <t>PCR</t> confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.
    Polymerase Chain Reaction Pcr Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr taq polymerase/product/Thermo Fisher
    Average 94 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr taq polymerase - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    94
    Millipore polymerase chain reaction pcr reaction solution reaction solution
    Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated <t>RNA</t> samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) <t>PCR</t> confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.
    Polymerase Chain Reaction Pcr Reaction Solution Reaction Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr reaction solution reaction solution/product/Millipore
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr reaction solution reaction solution - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    90
    Promega polymerase chain reaction pcr amplification buffer
    Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated <t>RNA</t> samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) <t>PCR</t> confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.
    Polymerase Chain Reaction Pcr Amplification Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr amplification buffer/product/Promega
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr amplification buffer - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    Detection of nptII gene in samples (Results of PCR products of primer pairs (nptIIf-nptIIr)) M: 100 bp DNA marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of nptII gene in samples (Results of PCR products of primer pairs (nptIIf-nptIIr)) M: 100 bp DNA marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of epsps gene in some samples tested (Results of PCR products of primer pairs (GMO9/GMO5)) M: 100 bp DNA marker, B: negative control, lanes A1–M10: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in some samples tested (Results of PCR products of primer pairs (GMO9/GMO5)) M: 100 bp DNA marker, B: negative control, lanes A1–M10: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of epsps gene in samples (Results of PCR products of primer pairs: GMO5, GMO9) M: 100 bp DNA marker, B: negative control, lanes A1–P2: Tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in samples (Results of PCR products of primer pairs: GMO5, GMO9) M: 100 bp DNA marker, B: negative control, lanes A1–P2: Tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of lectin gene in soybean samples tested (A1, A2, A3, A4, A5, and A6) (Results of PCR products of primer pair (GM03/GM04)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of lectin gene in soybean samples tested (A1, A2, A3, A4, A5, and A6) (Results of PCR products of primer pair (GM03/GM04)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of 35S promoter in samples (Results of PCR products of primer pairs p35S-cf3 and p35S-cf4), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of 35S promoter in samples (Results of PCR products of primer pairs p35S-cf3 and p35S-cf4), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of zein gene in maize samples tested (M1, M2, M3, M5, M6, M7, M8, M9, M10, M11, M12, M13, M14, M15, and M16) (Results of PCR products of primer pair zein3/zein4), M: 100 bp DNA marker, B: negative control, lanes A1–P2: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of zein gene in maize samples tested (M1, M2, M3, M5, M6, M7, M8, M9, M10, M11, M12, M13, M14, M15, and M16) (Results of PCR products of primer pair zein3/zein4), M: 100 bp DNA marker, B: negative control, lanes A1–P2: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of nos terminator in samples (Results of PCR products of primer pairs HA-nos118-f/HA-nos118-r), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of nos terminator in samples (Results of PCR products of primer pairs HA-nos118-f/HA-nos118-r), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of epsps gene in some samples tested by nested PCR (Results of PCR products of primer pairs (GMO8/GMO7)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in some samples tested by nested PCR (Results of PCR products of primer pairs (GMO8/GMO7)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Nested PCR, Polymerase Chain Reaction, Marker, Negative Control

    Ligase inhibitor treatment blocked cccDNA formation in nuclear extract. (A) Demonstration of the in vitro cccDNA formation assay. The indicated amount of purified DHBV rcDNA were incubated with HepG2 nuclear extract as described in Materials and Methods. After the in vitro cccDNA formation reaction, total DNA were extracted and subjected to DHBV Southern blot analysis (top panel) and cccDNA-specific PCR assay (bottom panel). The EcoRI-linearized DHBV unit-length DNA was used as marker in Southern blot and as PCR positive template in cccDNA PCR assay (lane 2). 1 ng of rcDNA alone (lane 3) and nuclear extract alone (lane 8) served as PCR negative controls. (B) The in vitro DHBV cccDNA formation reaction was treated with DNA ligases inhibitor L1 (20 μM), L25 (20 μM), or L189 (50 μM), or DMSO solvent, and cccDNA was detected by PCR (lanes 3–6). DHBV cccDNA purified from Dstet5 cells was used as PCR positive control (lane 1). 1 ng of rcDNA without incubation with nuclear extract (lane 7) or without PCR reaction (lane 8) served as negative controls.

    Journal: PLoS Pathogens

    Article Title: The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation

    doi: 10.1371/journal.ppat.1006784

    Figure Lengend Snippet: Ligase inhibitor treatment blocked cccDNA formation in nuclear extract. (A) Demonstration of the in vitro cccDNA formation assay. The indicated amount of purified DHBV rcDNA were incubated with HepG2 nuclear extract as described in Materials and Methods. After the in vitro cccDNA formation reaction, total DNA were extracted and subjected to DHBV Southern blot analysis (top panel) and cccDNA-specific PCR assay (bottom panel). The EcoRI-linearized DHBV unit-length DNA was used as marker in Southern blot and as PCR positive template in cccDNA PCR assay (lane 2). 1 ng of rcDNA alone (lane 3) and nuclear extract alone (lane 8) served as PCR negative controls. (B) The in vitro DHBV cccDNA formation reaction was treated with DNA ligases inhibitor L1 (20 μM), L25 (20 μM), or L189 (50 μM), or DMSO solvent, and cccDNA was detected by PCR (lanes 3–6). DHBV cccDNA purified from Dstet5 cells was used as PCR positive control (lane 1). 1 ng of rcDNA without incubation with nuclear extract (lane 7) or without PCR reaction (lane 8) served as negative controls.

    Article Snippet: The PCR reaction was assembled by mixing 0.5 μl DNA sample, 12.5 μl 2× PCR buffer (Clontech), 1 μl of 20 μM forward primer (5’-GCCAAGATAATGATTAAACCACG-3’), 1 μl of 20 μM reverse primer (5’-TCATACACATTGGCTAAGGCTC-3’), 0.5 μl Terra polymerase (Clontech), and 9.5 μl H2O.

    Techniques: In Vitro, Tube Formation Assay, Purification, Incubation, Southern Blot, Polymerase Chain Reaction, Marker, Positive Control

    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

    Journal: Osong Public Health and Research Perspectives

    Article Title: Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients

    doi: 10.1016/j.phrp.2013.09.005

    Figure Lengend Snippet: Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

    Article Snippet: The DNA amplification was performed using 15 μL of solution containing 10 ng of genomic DNA, 1 U of i-Max II DNA polymerase (iNtRON Biotechnology, Gyeonggi-do, Korea), 2.5 mM deoxyribonucleotide triphosphate mixture, 10× polymerase chain reaction (PCR) buffer, 10 pmol of each primer (Bioneer, Daejeon, Korea), 5× betaine (Sigma Aldrich, St. Louis, MO, USA), and distilled sterilized water.

    Techniques: Amplification, Polymerase Chain Reaction, DNA Sequencing

    Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated RNA samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) PCR confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Protein S is inducible by interleukin 4 in T cells and inhibits lymphoid cell procoagulant activity

    doi:

    Figure Lengend Snippet: Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated RNA samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) PCR confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.

    Article Snippet: DD reactions contained 20 ng of reverse-transcribed RNA, 1 μM of each primer, 1× PCR buffer (Boehringer Mannheim), all four dNTPs (each at 2 μM), 1 unit of Taq DNA polymerase (Boehringer Mannheim), and 1 μl of sequencing-grade 35 S-labeled dATP (New England Nuclear/DuPont) in a final volume of 20 μl.

    Techniques: Activation Assay, Polymerase Chain Reaction, Isolation, Cell Culture, Clone Assay, Sequencing