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  • 99
    New England Biolabs phusion pcr mix
    Phusion Pcr Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction pcr mixture
    Polymerase Chain Reaction Pcr Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kaneka Corp standard polymerase chain reaction pcr mix
    Standard Polymerase Chain Reaction Pcr Mix, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche polymerase chain reaction pcr mixture
    Polymerase Chain Reaction Pcr Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech polymerase chain reaction pcr mix kit
    Polymerase Chain Reaction Pcr Mix Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polymerase chain reaction pcr mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Polymerase Chain Reaction Pcr Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science sensimix polymerase chain reaction mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Sensimix Polymerase Chain Reaction Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer polymerase chain reaction pcr mixture
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Polymerase Chain Reaction Pcr Mixture, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybrgreen polymerase chain reaction pcr mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Sybrgreen Polymerase Chain Reaction Pcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixture, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare polymerase chain reaction pcr nucleotide mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Nucleotide Mix, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr pcr polymerase chain reaction master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Sybr Pcr Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Arraystar polymerase chain reaction pcr master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Master Mix, supplied by Arraystar, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene quantitative polymerase chain reaction master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Quantitative Polymerase Chain Reaction Master Mix, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction pcr master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kaneka Corp polymerase chain reaction pcr master mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Master Mix, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science pcr mixture
    ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: <t>cDNA</t> as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative <t>PCR</t> control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).
    Pcr Mixture, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 772 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems polymerase chain reaction pcr mix
    ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: <t>cDNA</t> as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative <t>PCR</t> control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).
    Polymerase Chain Reaction Pcr Mix, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr reaction mix
    ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: <t>cDNA</t> as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative <t>PCR</t> control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).
    Polymerase Chain Reaction Pcr Reaction Mix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr green mix polymerase chain reaction pcr kit
    ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: <t>cDNA</t> as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative <t>PCR</t> control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).
    Sybr Green Mix Polymerase Chain Reaction Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega polymerase chain reaction pcr master mix
    ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: <t>cDNA</t> as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative <t>PCR</t> control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).
    Polymerase Chain Reaction Pcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by PCR using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M DNA marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2

    doi: 10.1007/s11626-013-9641-1

    Figure Lengend Snippet: Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by PCR using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M DNA marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.

    Article Snippet: Two-hundred nanograms of DNA (for pSV3 neo plasmid DNA 10 ng) were diluted in a 25-μl polymerase chain reaction (PCR) mix (Sigma–Aldrich).

    Techniques: Expressing, Amplification, Polymerase Chain Reaction, Staining, Marker, Negative Control, Immunohistochemistry, Western Blot, SDS Page

    Identification of SV40 transformation. ( A ) Genomic DNA in the primary and immortalized dental papilla mesenchymal cells was isolated and amplified by the SV40 specific primer. PCR products were run on an agarose gel and stained with ethidium bromide. Lane 1 , DNA marker (Low DNA mass ladder, Invitrogen); lane 2 , control; lane 3 , primary dental papilla mesenchymal cells; lane 4 , immortalized cells. ( B , C ) Immunohistochemical staining with antibody against simian virus 40 large T-antigen (SV40 T-Ag) in iMDP-3 cells and immunolabeling of SV40 protein was mostly present in the nucleus ( green color ; B ) whereas immunostaining was not seen in the primary cells ( C ). The cells were stained with DAPI for the nucleus. Con control, Pri primary, Imm immortalized.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2

    doi: 10.1007/s11626-013-9641-1

    Figure Lengend Snippet: Identification of SV40 transformation. ( A ) Genomic DNA in the primary and immortalized dental papilla mesenchymal cells was isolated and amplified by the SV40 specific primer. PCR products were run on an agarose gel and stained with ethidium bromide. Lane 1 , DNA marker (Low DNA mass ladder, Invitrogen); lane 2 , control; lane 3 , primary dental papilla mesenchymal cells; lane 4 , immortalized cells. ( B , C ) Immunohistochemical staining with antibody against simian virus 40 large T-antigen (SV40 T-Ag) in iMDP-3 cells and immunolabeling of SV40 protein was mostly present in the nucleus ( green color ; B ) whereas immunostaining was not seen in the primary cells ( C ). The cells were stained with DAPI for the nucleus. Con control, Pri primary, Imm immortalized.

    Article Snippet: Two-hundred nanograms of DNA (for pSV3 neo plasmid DNA 10 ng) were diluted in a 25-μl polymerase chain reaction (PCR) mix (Sigma–Aldrich).

    Techniques: Transformation Assay, Isolation, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Marker, Immunohistochemistry, Immunolabeling, Immunostaining

    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

    Journal: BioMed Research International

    Article Title: Population Abundance of Potentially Pathogenic Organisms in Intestinal Microbiome of Jungle Crow (Corvus macrorhynchos) Shown with 16S rRNA Gene-Based Microbial Community Analysis

    doi: 10.1155/2013/438956

    Figure Lengend Snippet: Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

    Article Snippet: PCR Conditions Twenty-five to fifty nanograms of isolated DNA were transferred to a PCR tube containing 50 μ L of a polymerase chain reaction (PCR) mixture containing LA Taq DNA polymerase (TaKaRa Bio, Shiga, Japan).

    Techniques: Amplification, Polymerase Chain Reaction, Marker

    miR-92a-3p downregulates NF2 mRNA and protein expression by interacting with its conserved MRE within the NF2 −3′UTR. (A) Semi-quantitative reverse transcription-polymerase chain reaction (semi-RT-qPCR), (B) quantitative RT-PCR (RT-qPCR) and (C) western blotting detection of NF2 /Merlin expression levels in HCT116 cells transfected with empty vector or miR-92a expression construct. (D) RT-qPCR and (E) western blotting detection of NF2 /Merlin expression in HCT116 cells co-transfected with pmR-ZsGreen1 and negative control target protector (TPneg), or with miR-92a expression construct and miR-92a-target protector (TP-92a). All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Journal: Oncology Reports

    Article Title: MicroRNA-92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells

    doi: 10.3892/or.2019.7020

    Figure Lengend Snippet: miR-92a-3p downregulates NF2 mRNA and protein expression by interacting with its conserved MRE within the NF2 −3′UTR. (A) Semi-quantitative reverse transcription-polymerase chain reaction (semi-RT-qPCR), (B) quantitative RT-PCR (RT-qPCR) and (C) western blotting detection of NF2 /Merlin expression levels in HCT116 cells transfected with empty vector or miR-92a expression construct. (D) RT-qPCR and (E) western blotting detection of NF2 /Merlin expression in HCT116 cells co-transfected with pmR-ZsGreen1 and negative control target protector (TPneg), or with miR-92a expression construct and miR-92a-target protector (TP-92a). All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Article Snippet: The 3′UTR of human NF2 isoform I (NM_000268.3) and the pre-miR-92a-1 gene (NR_029508.1) were amplified in a polymerase chain reaction (PCR) reaction mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer; Clontech Laboratories, Inc., Mountain View, CA, USA), 0.125 µM of each deoxynucleoside triphosphate (dNTPs) (Promega Corporation, Madison, WI, USA), 2 µM each of the forward and reverse primers, 1X Taq polymerase (Titanium® Taq polymerase; Clontech Laboratories, Inc.) and wild-type human genomic DNA template available in the Disease Molecular Biology and Epigenetics Laboratory of the National Institute of Molecular Biology and Biotechnology (DMBEL-NIMBB).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation, Construct, Negative Control, Standard Deviation

    ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: cDNA as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative PCR control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).

    Journal: Open Biology

    Article Title: The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering

    doi: 10.1098/rsob.160212

    Figure Lengend Snippet: ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: cDNA as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative PCR control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).

    Article Snippet: For each sample, 2 µl of reverse transcribed cDNA was used as a template in a 25 µl total volume PCR mixture and amplified using MyTaq master mix (Bioline, UK) using the following cycling conditions: 94°C/30 s, followed by 35 cycles of 94°C/10 s, 53°C/10 s, 72°C/10 s and a final single 72°C/30 s cycle using the primers listed in electronic supplementary material, table S2.

    Techniques: Positive Control, Polymerase Chain Reaction