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  • 94
    New England Biolabs pcr cleanup kit
    Pcr Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 83 article reviews
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    90
    Thermo Fisher pcr cleanup kit
    Construction of DENV by Gibson assembly. (A) Assembly schemes of DENV infectious <t>DNA.</t> CMV, CMV promoter; HDV, HDV ribozyme; SV40PA, polyadenylation signal. (Left) Assembly scheme for DENV2-16681 and DENV4-H241; (right) assembly scheme for DENV4-v17. (B) The comparison between the foci of the original virus stocks and the foci of viruses recovered through Gibson assembly. The number of DENV <t>PCR</t> products assembled (# PCR) is shown. (C) PCR products for sequencing of virus recovered from transfected 293T cells were generated from cDNA and not contaminated assembled DNA. +, viral RNA was treated with RNase A during cDNA synthesis. (D) Comparison between sequence heterogeneities of the original v17 strain, v17 recovered by our method, and v17 cultured in C6/36 cells. The heterogeneous positions are highlighted in yellow.
    Pcr Cleanup Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen qiaquick polymerase chain reaction pcr cleanup kit
    Construction of DENV by Gibson assembly. (A) Assembly schemes of DENV infectious <t>DNA.</t> CMV, CMV promoter; HDV, HDV ribozyme; SV40PA, polyadenylation signal. (Left) Assembly scheme for DENV2-16681 and DENV4-H241; (right) assembly scheme for DENV4-v17. (B) The comparison between the foci of the original virus stocks and the foci of viruses recovered through Gibson assembly. The number of DENV <t>PCR</t> products assembled (# PCR) is shown. (C) PCR products for sequencing of virus recovered from transfected 293T cells were generated from cDNA and not contaminated assembled DNA. +, viral RNA was treated with RNase A during cDNA synthesis. (D) Comparison between sequence heterogeneities of the original v17 strain, v17 recovered by our method, and v17 cultured in C6/36 cells. The heterogeneous positions are highlighted in yellow.
    Qiaquick Polymerase Chain Reaction Pcr Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 7 article reviews
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    Millipore genelute polymerase chain reaction pcr cleanup kit
    Construction of DENV by Gibson assembly. (A) Assembly schemes of DENV infectious <t>DNA.</t> CMV, CMV promoter; HDV, HDV ribozyme; SV40PA, polyadenylation signal. (Left) Assembly scheme for DENV2-16681 and DENV4-H241; (right) assembly scheme for DENV4-v17. (B) The comparison between the foci of the original virus stocks and the foci of viruses recovered through Gibson assembly. The number of DENV <t>PCR</t> products assembled (# PCR) is shown. (C) PCR products for sequencing of virus recovered from transfected 293T cells were generated from cDNA and not contaminated assembled DNA. +, viral RNA was treated with RNase A during cDNA synthesis. (D) Comparison between sequence heterogeneities of the original v17 strain, v17 recovered by our method, and v17 cultured in C6/36 cells. The heterogeneous positions are highlighted in yellow.
    Genelute Polymerase Chain Reaction Pcr Cleanup Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    MACHEREY NAGEL pcr cleanup kit
    Verification of the integration of pOSV802 in S. coelicolor M145, S. lividans TK23, and S. albus J1074 chromosomes. (A) Principle of the <t>PCR</t> verification of the integration of the pOSV801 to pOSV812 vectors in the Streptomyces chromosomes (PCR 1 and PCR 2) (PCR 3, PCR verification before excision of modules 1 to 3). (B) PCR fragments obtained by PCR 1 ( attL region; expected sizes, 913 bp for M145 and TK23, 888 bp for J1074) and by PCR 2 ( attR region; expected sizes, 911 bp for M145 and TK23, 907 bp for J1074) on the three Streptomyces strains bearing pOSV802. No PCR amplification is expected when the genomic <t>DNA</t> of the wild-type Streptomyces strains is used as the matrix. MW corresponds to the molecular weight ladder (Thermo Scientific GeneRuler DNA ladder mix).
    Pcr Cleanup Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 707 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega pcr cleanup kit
    <t>PA3719</t> and MexR control of in vitro mexA transcription. (A) Impact of PA3719 on MexR repression of mexA transcription. Phage T7 RNA polymerase-based in vitro transcription reactions were initiated from a 836-bp <t>PCR-generated</t> template (250 ng) carrying a T7 promoter and mexA sequences extending from the MexR operator to 585 bp into the mexA gene (lanes 1 to 4). The results for control reactions with a T7 promoter-containing DNA template, pTRI-Xef, provided with the T7 in vitro transcription kit, are shown in lanes 5 to 7. Reactions were carried out at 37°C for 3 h, and RNA was purified and subjected to electrophoresis prior to visualization with ethidium bromide. MexR (1 μM) and PA3719 (150 nM [+ 1 ] or 500 nM [+ 2] ) were included in the reaction mixtures as indicated. Equal volumes of RNA were loaded in each instance. The following amounts of RNA were produced: lane 1, 4.2 μg; lane 2, 1.1 μg; lane 3, 2.0 μg; lane 4, 4.2 μg; lane 5, 1.7 μg; lane 6, 1.6 μg; and lane 7, 1.6 μg. (B) Impact of PA3719 on mexA transcription. In vitro transcription of the mex (lanes 1 to 3) and pTRI-Xef (lanes 4 to 6) templates was carried out as described above with or without PA3719 (500 nM [+ 2 ] or 2 μM [+ 3 ]), as indicated. The following amounts of RNA were produced: lane 1, 4.4 μg; lane 2, 4.5 μg; lane 3, 4.6 μg; lane 4, 4.6 μg; lane 5, 4.6 μg; and lane 6, 4.4 μg. (C) Differential impact of PA3719 on the repressor activities of MexR and MexR I104F . In vitro transcription of the mex (lanes 1 to 5) and pTRI-Xef (lanes 6 to 8) templates was carried out as described above with or without PA3719 (500 nM) and MexR/MexR I104F (1 μM), as indicated. The following amounts of RNA were produced: lane 1, 4.2 μg; lane 2, 0.8 μg; lane 3, 3.8 μg; lane 4, 0.9 μg; lane 5, 1.9 μg; lane 6, 6.6 μg; lane 7, 6.5 μg; and lane 8, 6.4 μg. RNA was quantitated prior to loading, and the values reported reflect the amount loaded and not the total amount produced. Because reaction, recovery, and loading volumes were kept constant throughout, these values accurately reflect the relative transcript levels produced in each instance.
    Pcr Cleanup Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Axygen pcr cleanup kit
    Phenotypes of the pre6 mutants and the PRE6 overexpression plants. (A) A diagram showing the <t>T-DNA</t> insertion sites in the pre6-1 and pre6-2 mutants. (B) Expression of PRE6 transcript in the pre6 mutants ( pre6-1 and pre6-2 ) and transgenic plants overexpressing PRE6 ( 35S:PRE6-1 and 35S:PRE6-2 ). RNA was isolated from 14-day-old seedlings, and <t>RT-PCR</t> was used to examine the expression of PRE6 . The expression of ACT2 was used as a control. (C) Photographs of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Sterilized seeds were grown on vertically placed 1/2 MS plates in darkness, and photographs were taken under a microscope. (D) Hypocotyl length of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Photographs were taken under a microscope, and ImageJ software was used to measure the hypocotyl length. Data represent the mean ± SD of 33–35 seedlings. ∗ Significantly different from the Col wild type seedlings ( p
    Pcr Cleanup Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 94/100, based on 712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA pcr cleanup kit
    Phenotypes of the pre6 mutants and the PRE6 overexpression plants. (A) A diagram showing the <t>T-DNA</t> insertion sites in the pre6-1 and pre6-2 mutants. (B) Expression of PRE6 transcript in the pre6 mutants ( pre6-1 and pre6-2 ) and transgenic plants overexpressing PRE6 ( 35S:PRE6-1 and 35S:PRE6-2 ). RNA was isolated from 14-day-old seedlings, and <t>RT-PCR</t> was used to examine the expression of PRE6 . The expression of ACT2 was used as a control. (C) Photographs of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Sterilized seeds were grown on vertically placed 1/2 MS plates in darkness, and photographs were taken under a microscope. (D) Hypocotyl length of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Photographs were taken under a microscope, and ImageJ software was used to measure the hypocotyl length. Data represent the mean ± SD of 33–35 seedlings. ∗ Significantly different from the Col wild type seedlings ( p
    Pcr Cleanup Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche pcr cleanup kit
    Phenotypes of the pre6 mutants and the PRE6 overexpression plants. (A) A diagram showing the <t>T-DNA</t> insertion sites in the pre6-1 and pre6-2 mutants. (B) Expression of PRE6 transcript in the pre6 mutants ( pre6-1 and pre6-2 ) and transgenic plants overexpressing PRE6 ( 35S:PRE6-1 and 35S:PRE6-2 ). RNA was isolated from 14-day-old seedlings, and <t>RT-PCR</t> was used to examine the expression of PRE6 . The expression of ACT2 was used as a control. (C) Photographs of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Sterilized seeds were grown on vertically placed 1/2 MS plates in darkness, and photographs were taken under a microscope. (D) Hypocotyl length of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Photographs were taken under a microscope, and ImageJ software was used to measure the hypocotyl length. Data represent the mean ± SD of 33–35 seedlings. ∗ Significantly different from the Col wild type seedlings ( p
    Pcr Cleanup Kit, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    tiangen biotech co pcr cleanup kits
    Electrophoresis of <t>PCR</t> products. Lane M: <t>DNA</t> marker; lanes 1–3: genomic DNA of strain 1-L-29 gfp ; 4: plasmid pGFP22; 5: genomic DNA of strain 1-L-29.
    Pcr Cleanup Kits, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific pcr cleanup kit
    Electrophoresis of <t>PCR</t> products. Lane M: <t>DNA</t> marker; lanes 1–3: genomic DNA of strain 1-L-29 gfp ; 4: plasmid pGFP22; 5: genomic DNA of strain 1-L-29.
    Pcr Cleanup Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    TaKaRa pcr cleanup kit
    TGIRT ssDNA-seq workflow. The target DNA (2–50 ng in this work) is treated with alkaline phosphatase to remove 3′ phosphates and heat denatured prior to DNA-seq library construction. The resulting ssDNAs with 3′ OH termini are then used for TGIRT template-switching DNA synthesis coupled to Illumina read 2 reverse (R2R) DNA-seq adapter addition. In this novel reaction, the TGIRT-III enzyme (InGex) binds first to a synthetic 34-bp R2 RNA/R2R DNA heteroduplex in which the R2R DNA primer has a single nucleotide 3′ overhang that can direct TGIRT template switching by base pairing to the 3′ nucleotide of a target DNA strand. For minimally biased library preparation, the 3′-overhang nucleotide is an equimolar mixture of A, C, G, and T (denoted N). A 3′-blocking group (C3 spacer; 3′SpC3) is attached to the end of the R2 RNA oligonucleotide to prevent template-switching to that RNA. After the TGIRT enzyme extends the DNA primer to produce a DNA copy of the target DNA strand with an R2R adapter seamlessly linked to its 5′ end, a 5′ adenylated (App) Illumina read 1 reverse <t>(R1R)</t> adapter is added to its 3′ end by single-stranded DNA ligation using a Thermostable 5′ AppDNA/RNA ligase (New England Biolabs). In the workflow shown, an unique molecular identifier (UMI) is positioned at the 5′ end of the R1R DNA oligonucleotide. A final <t>PCR</t> step adds flow cell capture sites and barcodes for Illumina sequencing and sample multiplexing.
    Pcr Cleanup Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen ultraclean pcr cleanup kit
    TGIRT ssDNA-seq workflow. The target DNA (2–50 ng in this work) is treated with alkaline phosphatase to remove 3′ phosphates and heat denatured prior to DNA-seq library construction. The resulting ssDNAs with 3′ OH termini are then used for TGIRT template-switching DNA synthesis coupled to Illumina read 2 reverse (R2R) DNA-seq adapter addition. In this novel reaction, the TGIRT-III enzyme (InGex) binds first to a synthetic 34-bp R2 RNA/R2R DNA heteroduplex in which the R2R DNA primer has a single nucleotide 3′ overhang that can direct TGIRT template switching by base pairing to the 3′ nucleotide of a target DNA strand. For minimally biased library preparation, the 3′-overhang nucleotide is an equimolar mixture of A, C, G, and T (denoted N). A 3′-blocking group (C3 spacer; 3′SpC3) is attached to the end of the R2 RNA oligonucleotide to prevent template-switching to that RNA. After the TGIRT enzyme extends the DNA primer to produce a DNA copy of the target DNA strand with an R2R adapter seamlessly linked to its 5′ end, a 5′ adenylated (App) Illumina read 1 reverse <t>(R1R)</t> adapter is added to its 3′ end by single-stranded DNA ligation using a Thermostable 5′ AppDNA/RNA ligase (New England Biolabs). In the workflow shown, an unique molecular identifier (UMI) is positioned at the 5′ end of the R1R DNA oligonucleotide. A final <t>PCR</t> step adds flow cell capture sites and barcodes for Illumina sequencing and sample multiplexing.
    Ultraclean Pcr Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 281 article reviews
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    99
    New England Biolabs monarch pcr cleanup kit
    TGIRT ssDNA-seq workflow. The target DNA (2–50 ng in this work) is treated with alkaline phosphatase to remove 3′ phosphates and heat denatured prior to DNA-seq library construction. The resulting ssDNAs with 3′ OH termini are then used for TGIRT template-switching DNA synthesis coupled to Illumina read 2 reverse (R2R) DNA-seq adapter addition. In this novel reaction, the TGIRT-III enzyme (InGex) binds first to a synthetic 34-bp R2 RNA/R2R DNA heteroduplex in which the R2R DNA primer has a single nucleotide 3′ overhang that can direct TGIRT template switching by base pairing to the 3′ nucleotide of a target DNA strand. For minimally biased library preparation, the 3′-overhang nucleotide is an equimolar mixture of A, C, G, and T (denoted N). A 3′-blocking group (C3 spacer; 3′SpC3) is attached to the end of the R2 RNA oligonucleotide to prevent template-switching to that RNA. After the TGIRT enzyme extends the DNA primer to produce a DNA copy of the target DNA strand with an R2R adapter seamlessly linked to its 5′ end, a 5′ adenylated (App) Illumina read 1 reverse <t>(R1R)</t> adapter is added to its 3′ end by single-stranded DNA ligation using a Thermostable 5′ AppDNA/RNA ligase (New England Biolabs). In the workflow shown, an unique molecular identifier (UMI) is positioned at the 5′ end of the R1R DNA oligonucleotide. A final <t>PCR</t> step adds flow cell capture sites and barcodes for Illumina sequencing and sample multiplexing.
    Monarch Pcr Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore montage pcr cleanup kit
    TGIRT ssDNA-seq workflow. The target DNA (2–50 ng in this work) is treated with alkaline phosphatase to remove 3′ phosphates and heat denatured prior to DNA-seq library construction. The resulting ssDNAs with 3′ OH termini are then used for TGIRT template-switching DNA synthesis coupled to Illumina read 2 reverse (R2R) DNA-seq adapter addition. In this novel reaction, the TGIRT-III enzyme (InGex) binds first to a synthetic 34-bp R2 RNA/R2R DNA heteroduplex in which the R2R DNA primer has a single nucleotide 3′ overhang that can direct TGIRT template switching by base pairing to the 3′ nucleotide of a target DNA strand. For minimally biased library preparation, the 3′-overhang nucleotide is an equimolar mixture of A, C, G, and T (denoted N). A 3′-blocking group (C3 spacer; 3′SpC3) is attached to the end of the R2 RNA oligonucleotide to prevent template-switching to that RNA. After the TGIRT enzyme extends the DNA primer to produce a DNA copy of the target DNA strand with an R2R adapter seamlessly linked to its 5′ end, a 5′ adenylated (App) Illumina read 1 reverse <t>(R1R)</t> adapter is added to its 3′ end by single-stranded DNA ligation using a Thermostable 5′ AppDNA/RNA ligase (New England Biolabs). In the workflow shown, an unique molecular identifier (UMI) is positioned at the 5′ end of the R1R DNA oligonucleotide. A final <t>PCR</t> step adds flow cell capture sites and barcodes for Illumina sequencing and sample multiplexing.
    Montage Pcr Cleanup Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen pcr cleanup kit
    FOXC1 binds to the Msx2 promoter in vivo. (A) Sequence analysis of upstream regulatory elements reveals the presence of a FOXC1 binding motif, indicated in bold, (TAAAT/CAAT) located in a conserved motif near the predicted Msx2 transcription start site of the mouse, rat and human genes. Small arrows correspond to the position of ChIP primers located in the promoter region or the coding region of mouse Msx2 . Nucleotide sequences of the Electrophoretic mobility shift assay (EMSA) probes for wild type (WT) and mutated (MUT) FOXC1 binding sites are indicated. (B) Chromatin immunoprecipitation assays confirm the binding of FOXC1 to the Msx2 promoter in vivo . Quantitative <t>PCR</t> (qPCR) was conducted on ChiP products isolated from 10T1/2 cells using antibodies recognizing FOXC1 or normal immunoglobulins (IgG). Primers were designed amplify regions in the promoter flanking the putative FOXC1 binding site or exon 2 of the mouse Msx2 gene. Amplification signals are presented a percentage compared to input chromatin fraction. (C) EMSAs demonstrate FOXC1 binding to <t>DNA</t> elements in the Msx2 promoter. Extracts from U2OS cells or cells transfected with FOXC1 were incubated with IR700-labeled oligonucleotides correspond to the WT or MUT FOXC1 binding sites. FOXC1-DNA complexes are indicated by the arrow.
    Pcr Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega wizard pcr cleanup kit
    FOXC1 binds to the Msx2 promoter in vivo. (A) Sequence analysis of upstream regulatory elements reveals the presence of a FOXC1 binding motif, indicated in bold, (TAAAT/CAAT) located in a conserved motif near the predicted Msx2 transcription start site of the mouse, rat and human genes. Small arrows correspond to the position of ChIP primers located in the promoter region or the coding region of mouse Msx2 . Nucleotide sequences of the Electrophoretic mobility shift assay (EMSA) probes for wild type (WT) and mutated (MUT) FOXC1 binding sites are indicated. (B) Chromatin immunoprecipitation assays confirm the binding of FOXC1 to the Msx2 promoter in vivo . Quantitative <t>PCR</t> (qPCR) was conducted on ChiP products isolated from 10T1/2 cells using antibodies recognizing FOXC1 or normal immunoglobulins (IgG). Primers were designed amplify regions in the promoter flanking the putative FOXC1 binding site or exon 2 of the mouse Msx2 gene. Amplification signals are presented a percentage compared to input chromatin fraction. (C) EMSAs demonstrate FOXC1 binding to <t>DNA</t> elements in the Msx2 promoter. Extracts from U2OS cells or cells transfected with FOXC1 were incubated with IR700-labeled oligonucleotides correspond to the WT or MUT FOXC1 binding sites. FOXC1-DNA complexes are indicated by the arrow.
    Wizard Pcr Cleanup Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Axygen axyprep pcr cleanup kit
    FOXC1 binds to the Msx2 promoter in vivo. (A) Sequence analysis of upstream regulatory elements reveals the presence of a FOXC1 binding motif, indicated in bold, (TAAAT/CAAT) located in a conserved motif near the predicted Msx2 transcription start site of the mouse, rat and human genes. Small arrows correspond to the position of ChIP primers located in the promoter region or the coding region of mouse Msx2 . Nucleotide sequences of the Electrophoretic mobility shift assay (EMSA) probes for wild type (WT) and mutated (MUT) FOXC1 binding sites are indicated. (B) Chromatin immunoprecipitation assays confirm the binding of FOXC1 to the Msx2 promoter in vivo . Quantitative <t>PCR</t> (qPCR) was conducted on ChiP products isolated from 10T1/2 cells using antibodies recognizing FOXC1 or normal immunoglobulins (IgG). Primers were designed amplify regions in the promoter flanking the putative FOXC1 binding site or exon 2 of the mouse Msx2 gene. Amplification signals are presented a percentage compared to input chromatin fraction. (C) EMSAs demonstrate FOXC1 binding to <t>DNA</t> elements in the Msx2 promoter. Extracts from U2OS cells or cells transfected with FOXC1 were incubated with IR700-labeled oligonucleotides correspond to the WT or MUT FOXC1 binding sites. FOXC1-DNA complexes are indicated by the arrow.
    Axyprep Pcr Cleanup Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 89/100, based on 394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega wizardsv pcr cleanup kit
    FOXC1 binds to the Msx2 promoter in vivo. (A) Sequence analysis of upstream regulatory elements reveals the presence of a FOXC1 binding motif, indicated in bold, (TAAAT/CAAT) located in a conserved motif near the predicted Msx2 transcription start site of the mouse, rat and human genes. Small arrows correspond to the position of ChIP primers located in the promoter region or the coding region of mouse Msx2 . Nucleotide sequences of the Electrophoretic mobility shift assay (EMSA) probes for wild type (WT) and mutated (MUT) FOXC1 binding sites are indicated. (B) Chromatin immunoprecipitation assays confirm the binding of FOXC1 to the Msx2 promoter in vivo . Quantitative <t>PCR</t> (qPCR) was conducted on ChiP products isolated from 10T1/2 cells using antibodies recognizing FOXC1 or normal immunoglobulins (IgG). Primers were designed amplify regions in the promoter flanking the putative FOXC1 binding site or exon 2 of the mouse Msx2 gene. Amplification signals are presented a percentage compared to input chromatin fraction. (C) EMSAs demonstrate FOXC1 binding to <t>DNA</t> elements in the Msx2 promoter. Extracts from U2OS cells or cells transfected with FOXC1 were incubated with IR700-labeled oligonucleotides correspond to the WT or MUT FOXC1 binding sites. FOXC1-DNA complexes are indicated by the arrow.
    Wizardsv Pcr Cleanup Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Construction of DENV by Gibson assembly. (A) Assembly schemes of DENV infectious DNA. CMV, CMV promoter; HDV, HDV ribozyme; SV40PA, polyadenylation signal. (Left) Assembly scheme for DENV2-16681 and DENV4-H241; (right) assembly scheme for DENV4-v17. (B) The comparison between the foci of the original virus stocks and the foci of viruses recovered through Gibson assembly. The number of DENV PCR products assembled (# PCR) is shown. (C) PCR products for sequencing of virus recovered from transfected 293T cells were generated from cDNA and not contaminated assembled DNA. +, viral RNA was treated with RNase A during cDNA synthesis. (D) Comparison between sequence heterogeneities of the original v17 strain, v17 recovered by our method, and v17 cultured in C6/36 cells. The heterogeneous positions are highlighted in yellow.

    Journal: Journal of Virology

    Article Title: A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput

    doi: 10.1128/JVI.02261-13

    Figure Lengend Snippet: Construction of DENV by Gibson assembly. (A) Assembly schemes of DENV infectious DNA. CMV, CMV promoter; HDV, HDV ribozyme; SV40PA, polyadenylation signal. (Left) Assembly scheme for DENV2-16681 and DENV4-H241; (right) assembly scheme for DENV4-v17. (B) The comparison between the foci of the original virus stocks and the foci of viruses recovered through Gibson assembly. The number of DENV PCR products assembled (# PCR) is shown. (C) PCR products for sequencing of virus recovered from transfected 293T cells were generated from cDNA and not contaminated assembled DNA. +, viral RNA was treated with RNase A during cDNA synthesis. (D) Comparison between sequence heterogeneities of the original v17 strain, v17 recovered by our method, and v17 cultured in C6/36 cells. The heterogeneous positions are highlighted in yellow.

    Article Snippet: All the PCR products were cleaned up using a PCR cleanup kit from Invitrogen before use in DNA assembly and sequencing.

    Techniques: Polymerase Chain Reaction, Sequencing, Transfection, Generated, Cell Culture

    Construction of a DENV library with randomized amino acids at position E1690. (A) Diagram showing how the randomization was achieved at the assembly site of two PCR products (dark gray and black) during Gibson ligation. The primer sites for PCR are depicted in letters, while the rest of DNA strands are represented by solid lines. The arrows represent the direction of repair by Phusion polymerase during the assembly reaction. (B) Chromatograms of sequences at prM847, E1690, and NS4B7016 of prM+E+NS4B virus with amino acid randomization at E1690. The randomized position in E and heterogeneous nucleotides in M and NS4B are highlighted in yellow. (C) Comparison between the foci of the DENV library (prM+LibE+NS4B) and the foci of the virus with the same genetic background without randomization at E1690 (prM+E+NS4B). The foci for each virus stock in duplicate wells are shown.

    Journal: Journal of Virology

    Article Title: A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput

    doi: 10.1128/JVI.02261-13

    Figure Lengend Snippet: Construction of a DENV library with randomized amino acids at position E1690. (A) Diagram showing how the randomization was achieved at the assembly site of two PCR products (dark gray and black) during Gibson ligation. The primer sites for PCR are depicted in letters, while the rest of DNA strands are represented by solid lines. The arrows represent the direction of repair by Phusion polymerase during the assembly reaction. (B) Chromatograms of sequences at prM847, E1690, and NS4B7016 of prM+E+NS4B virus with amino acid randomization at E1690. The randomized position in E and heterogeneous nucleotides in M and NS4B are highlighted in yellow. (C) Comparison between the foci of the DENV library (prM+LibE+NS4B) and the foci of the virus with the same genetic background without randomization at E1690 (prM+E+NS4B). The foci for each virus stock in duplicate wells are shown.

    Article Snippet: All the PCR products were cleaned up using a PCR cleanup kit from Invitrogen before use in DNA assembly and sequencing.

    Techniques: Polymerase Chain Reaction, Ligation

    Verification of the integration of pOSV802 in S. coelicolor M145, S. lividans TK23, and S. albus J1074 chromosomes. (A) Principle of the PCR verification of the integration of the pOSV801 to pOSV812 vectors in the Streptomyces chromosomes (PCR 1 and PCR 2) (PCR 3, PCR verification before excision of modules 1 to 3). (B) PCR fragments obtained by PCR 1 ( attL region; expected sizes, 913 bp for M145 and TK23, 888 bp for J1074) and by PCR 2 ( attR region; expected sizes, 911 bp for M145 and TK23, 907 bp for J1074) on the three Streptomyces strains bearing pOSV802. No PCR amplification is expected when the genomic DNA of the wild-type Streptomyces strains is used as the matrix. MW corresponds to the molecular weight ladder (Thermo Scientific GeneRuler DNA ladder mix).

    Journal: Applied and Environmental Microbiology

    Article Title: Modular and Integrative Vectors for Synthetic Biology Applications in Streptomyces spp.

    doi: 10.1128/AEM.00485-19

    Figure Lengend Snippet: Verification of the integration of pOSV802 in S. coelicolor M145, S. lividans TK23, and S. albus J1074 chromosomes. (A) Principle of the PCR verification of the integration of the pOSV801 to pOSV812 vectors in the Streptomyces chromosomes (PCR 1 and PCR 2) (PCR 3, PCR verification before excision of modules 1 to 3). (B) PCR fragments obtained by PCR 1 ( attL region; expected sizes, 913 bp for M145 and TK23, 888 bp for J1074) and by PCR 2 ( attR region; expected sizes, 911 bp for M145 and TK23, 907 bp for J1074) on the three Streptomyces strains bearing pOSV802. No PCR amplification is expected when the genomic DNA of the wild-type Streptomyces strains is used as the matrix. MW corresponds to the molecular weight ladder (Thermo Scientific GeneRuler DNA ladder mix).

    Article Snippet: DNA fragments were purified from agarose gels using the NucleoSpin gel and PCR cleanup kit from Macherey-Nagel.

    Techniques: Polymerase Chain Reaction, Amplification, Molecular Weight

    PA3719 and MexR control of in vitro mexA transcription. (A) Impact of PA3719 on MexR repression of mexA transcription. Phage T7 RNA polymerase-based in vitro transcription reactions were initiated from a 836-bp PCR-generated template (250 ng) carrying a T7 promoter and mexA sequences extending from the MexR operator to 585 bp into the mexA gene (lanes 1 to 4). The results for control reactions with a T7 promoter-containing DNA template, pTRI-Xef, provided with the T7 in vitro transcription kit, are shown in lanes 5 to 7. Reactions were carried out at 37°C for 3 h, and RNA was purified and subjected to electrophoresis prior to visualization with ethidium bromide. MexR (1 μM) and PA3719 (150 nM [+ 1 ] or 500 nM [+ 2] ) were included in the reaction mixtures as indicated. Equal volumes of RNA were loaded in each instance. The following amounts of RNA were produced: lane 1, 4.2 μg; lane 2, 1.1 μg; lane 3, 2.0 μg; lane 4, 4.2 μg; lane 5, 1.7 μg; lane 6, 1.6 μg; and lane 7, 1.6 μg. (B) Impact of PA3719 on mexA transcription. In vitro transcription of the mex (lanes 1 to 3) and pTRI-Xef (lanes 4 to 6) templates was carried out as described above with or without PA3719 (500 nM [+ 2 ] or 2 μM [+ 3 ]), as indicated. The following amounts of RNA were produced: lane 1, 4.4 μg; lane 2, 4.5 μg; lane 3, 4.6 μg; lane 4, 4.6 μg; lane 5, 4.6 μg; and lane 6, 4.4 μg. (C) Differential impact of PA3719 on the repressor activities of MexR and MexR I104F . In vitro transcription of the mex (lanes 1 to 5) and pTRI-Xef (lanes 6 to 8) templates was carried out as described above with or without PA3719 (500 nM) and MexR/MexR I104F (1 μM), as indicated. The following amounts of RNA were produced: lane 1, 4.2 μg; lane 2, 0.8 μg; lane 3, 3.8 μg; lane 4, 0.9 μg; lane 5, 1.9 μg; lane 6, 6.6 μg; lane 7, 6.5 μg; and lane 8, 6.4 μg. RNA was quantitated prior to loading, and the values reported reflect the amount loaded and not the total amount produced. Because reaction, recovery, and loading volumes were kept constant throughout, these values accurately reflect the relative transcript levels produced in each instance.

    Journal: Journal of Bacteriology

    Article Title: Protein Modulator of Multidrug Efflux Gene Expression in Pseudomonas aeruginosa ▿

    doi: 10.1128/JB.00543-07

    Figure Lengend Snippet: PA3719 and MexR control of in vitro mexA transcription. (A) Impact of PA3719 on MexR repression of mexA transcription. Phage T7 RNA polymerase-based in vitro transcription reactions were initiated from a 836-bp PCR-generated template (250 ng) carrying a T7 promoter and mexA sequences extending from the MexR operator to 585 bp into the mexA gene (lanes 1 to 4). The results for control reactions with a T7 promoter-containing DNA template, pTRI-Xef, provided with the T7 in vitro transcription kit, are shown in lanes 5 to 7. Reactions were carried out at 37°C for 3 h, and RNA was purified and subjected to electrophoresis prior to visualization with ethidium bromide. MexR (1 μM) and PA3719 (150 nM [+ 1 ] or 500 nM [+ 2] ) were included in the reaction mixtures as indicated. Equal volumes of RNA were loaded in each instance. The following amounts of RNA were produced: lane 1, 4.2 μg; lane 2, 1.1 μg; lane 3, 2.0 μg; lane 4, 4.2 μg; lane 5, 1.7 μg; lane 6, 1.6 μg; and lane 7, 1.6 μg. (B) Impact of PA3719 on mexA transcription. In vitro transcription of the mex (lanes 1 to 3) and pTRI-Xef (lanes 4 to 6) templates was carried out as described above with or without PA3719 (500 nM [+ 2 ] or 2 μM [+ 3 ]), as indicated. The following amounts of RNA were produced: lane 1, 4.4 μg; lane 2, 4.5 μg; lane 3, 4.6 μg; lane 4, 4.6 μg; lane 5, 4.6 μg; and lane 6, 4.4 μg. (C) Differential impact of PA3719 on the repressor activities of MexR and MexR I104F . In vitro transcription of the mex (lanes 1 to 5) and pTRI-Xef (lanes 6 to 8) templates was carried out as described above with or without PA3719 (500 nM) and MexR/MexR I104F (1 μM), as indicated. The following amounts of RNA were produced: lane 1, 4.2 μg; lane 2, 0.8 μg; lane 3, 3.8 μg; lane 4, 0.9 μg; lane 5, 1.9 μg; lane 6, 6.6 μg; lane 7, 6.5 μg; and lane 8, 6.4 μg. RNA was quantitated prior to loading, and the values reported reflect the amount loaded and not the total amount produced. Because reaction, recovery, and loading volumes were kept constant throughout, these values accurately reflect the relative transcript levels produced in each instance.

    Article Snippet: Annealing was achieved by incubating 800 pmol of each oligonucleotide in T4 ligation buffer at 95°C for 3 min and allowing the reaction mixture to cool at room temperature for 5 h, after which the 3′ PA3719 DNA was purified using a gel and PCR cleanup kit (Promega).

    Techniques: In Vitro, Polymerase Chain Reaction, Generated, Purification, Electrophoresis, Produced

    Phenotypes of the pre6 mutants and the PRE6 overexpression plants. (A) A diagram showing the T-DNA insertion sites in the pre6-1 and pre6-2 mutants. (B) Expression of PRE6 transcript in the pre6 mutants ( pre6-1 and pre6-2 ) and transgenic plants overexpressing PRE6 ( 35S:PRE6-1 and 35S:PRE6-2 ). RNA was isolated from 14-day-old seedlings, and RT-PCR was used to examine the expression of PRE6 . The expression of ACT2 was used as a control. (C) Photographs of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Sterilized seeds were grown on vertically placed 1/2 MS plates in darkness, and photographs were taken under a microscope. (D) Hypocotyl length of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Photographs were taken under a microscope, and ImageJ software was used to measure the hypocotyl length. Data represent the mean ± SD of 33–35 seedlings. ∗ Significantly different from the Col wild type seedlings ( p

    Journal: Frontiers in Plant Science

    Article Title: Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis

    doi: 10.3389/fpls.2017.01813

    Figure Lengend Snippet: Phenotypes of the pre6 mutants and the PRE6 overexpression plants. (A) A diagram showing the T-DNA insertion sites in the pre6-1 and pre6-2 mutants. (B) Expression of PRE6 transcript in the pre6 mutants ( pre6-1 and pre6-2 ) and transgenic plants overexpressing PRE6 ( 35S:PRE6-1 and 35S:PRE6-2 ). RNA was isolated from 14-day-old seedlings, and RT-PCR was used to examine the expression of PRE6 . The expression of ACT2 was used as a control. (C) Photographs of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Sterilized seeds were grown on vertically placed 1/2 MS plates in darkness, and photographs were taken under a microscope. (D) Hypocotyl length of the 5-day-old etiolated seedlings of the Col wild type, the pre6 mutants and the 35S:PRE6 transgenic plants. Photographs were taken under a microscope, and ImageJ software was used to measure the hypocotyl length. Data represent the mean ± SD of 33–35 seedlings. ∗ Significantly different from the Col wild type seedlings ( p

    Article Snippet: After washing, the DNA-protein cross-links obtained were reversed at 65°C for 12 h, and DNA was purified using PCR Cleanup Kit (Axygen) for PCR reactions.

    Techniques: Over Expression, Expressing, Transgenic Assay, Isolation, Reverse Transcription Polymerase Chain Reaction, Mass Spectrometry, Microscopy, Software

    Electrophoresis of PCR products. Lane M: DNA marker; lanes 1–3: genomic DNA of strain 1-L-29 gfp ; 4: plasmid pGFP22; 5: genomic DNA of strain 1-L-29.

    Journal: PLoS ONE

    Article Title: Isolation and characterization of Bacillus subtilis strain 1-L-29, an endophytic bacteria from Camellia oleifera with antimicrobial activity and efficient plant-root colonization

    doi: 10.1371/journal.pone.0232096

    Figure Lengend Snippet: Electrophoresis of PCR products. Lane M: DNA marker; lanes 1–3: genomic DNA of strain 1-L-29 gfp ; 4: plasmid pGFP22; 5: genomic DNA of strain 1-L-29.

    Article Snippet: PCR products were purified using Tiangen PCR Cleanup Kits, and sequenced on an ABI 3730 DNA Analyzer (ABI, CA, USA).

    Techniques: Electrophoresis, Polymerase Chain Reaction, Marker, Plasmid Preparation

    TGIRT ssDNA-seq workflow. The target DNA (2–50 ng in this work) is treated with alkaline phosphatase to remove 3′ phosphates and heat denatured prior to DNA-seq library construction. The resulting ssDNAs with 3′ OH termini are then used for TGIRT template-switching DNA synthesis coupled to Illumina read 2 reverse (R2R) DNA-seq adapter addition. In this novel reaction, the TGIRT-III enzyme (InGex) binds first to a synthetic 34-bp R2 RNA/R2R DNA heteroduplex in which the R2R DNA primer has a single nucleotide 3′ overhang that can direct TGIRT template switching by base pairing to the 3′ nucleotide of a target DNA strand. For minimally biased library preparation, the 3′-overhang nucleotide is an equimolar mixture of A, C, G, and T (denoted N). A 3′-blocking group (C3 spacer; 3′SpC3) is attached to the end of the R2 RNA oligonucleotide to prevent template-switching to that RNA. After the TGIRT enzyme extends the DNA primer to produce a DNA copy of the target DNA strand with an R2R adapter seamlessly linked to its 5′ end, a 5′ adenylated (App) Illumina read 1 reverse (R1R) adapter is added to its 3′ end by single-stranded DNA ligation using a Thermostable 5′ AppDNA/RNA ligase (New England Biolabs). In the workflow shown, an unique molecular identifier (UMI) is positioned at the 5′ end of the R1R DNA oligonucleotide. A final PCR step adds flow cell capture sites and barcodes for Illumina sequencing and sample multiplexing.

    Journal: Scientific Reports

    Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching

    doi: 10.1038/s41598-017-09064-w

    Figure Lengend Snippet: TGIRT ssDNA-seq workflow. The target DNA (2–50 ng in this work) is treated with alkaline phosphatase to remove 3′ phosphates and heat denatured prior to DNA-seq library construction. The resulting ssDNAs with 3′ OH termini are then used for TGIRT template-switching DNA synthesis coupled to Illumina read 2 reverse (R2R) DNA-seq adapter addition. In this novel reaction, the TGIRT-III enzyme (InGex) binds first to a synthetic 34-bp R2 RNA/R2R DNA heteroduplex in which the R2R DNA primer has a single nucleotide 3′ overhang that can direct TGIRT template switching by base pairing to the 3′ nucleotide of a target DNA strand. For minimally biased library preparation, the 3′-overhang nucleotide is an equimolar mixture of A, C, G, and T (denoted N). A 3′-blocking group (C3 spacer; 3′SpC3) is attached to the end of the R2 RNA oligonucleotide to prevent template-switching to that RNA. After the TGIRT enzyme extends the DNA primer to produce a DNA copy of the target DNA strand with an R2R adapter seamlessly linked to its 5′ end, a 5′ adenylated (App) Illumina read 1 reverse (R1R) adapter is added to its 3′ end by single-stranded DNA ligation using a Thermostable 5′ AppDNA/RNA ligase (New England Biolabs). In the workflow shown, an unique molecular identifier (UMI) is positioned at the 5′ end of the R1R DNA oligonucleotide. A final PCR step adds flow cell capture sites and barcodes for Illumina sequencing and sample multiplexing.

    Article Snippet: After cleaning up by using a Nucleospin Gel and PCR cleanup kit (Clontech), a 5′App/3′-CpSp blocked R1R adapter with a 13-nt UMI at its 5′ end (denoted R1R-UMI) was ligated to the 3′ end of the cDNA by using Thermostable 5′ AppDNA/RNA Ligase as specified by manufacturer’s protocol (New England Biolabs).

    Techniques: DNA Sequencing, DNA Synthesis, Blocking Assay, DNA Ligation, Polymerase Chain Reaction, Flow Cytometry, Sequencing, Multiplexing

    FOXC1 binds to the Msx2 promoter in vivo. (A) Sequence analysis of upstream regulatory elements reveals the presence of a FOXC1 binding motif, indicated in bold, (TAAAT/CAAT) located in a conserved motif near the predicted Msx2 transcription start site of the mouse, rat and human genes. Small arrows correspond to the position of ChIP primers located in the promoter region or the coding region of mouse Msx2 . Nucleotide sequences of the Electrophoretic mobility shift assay (EMSA) probes for wild type (WT) and mutated (MUT) FOXC1 binding sites are indicated. (B) Chromatin immunoprecipitation assays confirm the binding of FOXC1 to the Msx2 promoter in vivo . Quantitative PCR (qPCR) was conducted on ChiP products isolated from 10T1/2 cells using antibodies recognizing FOXC1 or normal immunoglobulins (IgG). Primers were designed amplify regions in the promoter flanking the putative FOXC1 binding site or exon 2 of the mouse Msx2 gene. Amplification signals are presented a percentage compared to input chromatin fraction. (C) EMSAs demonstrate FOXC1 binding to DNA elements in the Msx2 promoter. Extracts from U2OS cells or cells transfected with FOXC1 were incubated with IR700-labeled oligonucleotides correspond to the WT or MUT FOXC1 binding sites. FOXC1-DNA complexes are indicated by the arrow.

    Journal: PLoS ONE

    Article Title: Initiation of Early Osteoblast Differentiation Events through the Direct Transcriptional Regulation of Msx2 by FOXC1

    doi: 10.1371/journal.pone.0049095

    Figure Lengend Snippet: FOXC1 binds to the Msx2 promoter in vivo. (A) Sequence analysis of upstream regulatory elements reveals the presence of a FOXC1 binding motif, indicated in bold, (TAAAT/CAAT) located in a conserved motif near the predicted Msx2 transcription start site of the mouse, rat and human genes. Small arrows correspond to the position of ChIP primers located in the promoter region or the coding region of mouse Msx2 . Nucleotide sequences of the Electrophoretic mobility shift assay (EMSA) probes for wild type (WT) and mutated (MUT) FOXC1 binding sites are indicated. (B) Chromatin immunoprecipitation assays confirm the binding of FOXC1 to the Msx2 promoter in vivo . Quantitative PCR (qPCR) was conducted on ChiP products isolated from 10T1/2 cells using antibodies recognizing FOXC1 or normal immunoglobulins (IgG). Primers were designed amplify regions in the promoter flanking the putative FOXC1 binding site or exon 2 of the mouse Msx2 gene. Amplification signals are presented a percentage compared to input chromatin fraction. (C) EMSAs demonstrate FOXC1 binding to DNA elements in the Msx2 promoter. Extracts from U2OS cells or cells transfected with FOXC1 were incubated with IR700-labeled oligonucleotides correspond to the WT or MUT FOXC1 binding sites. FOXC1-DNA complexes are indicated by the arrow.

    Article Snippet: Qiagen PCR cleanup kit was used to isolate ChIP DNA.

    Techniques: In Vivo, Sequencing, Binding Assay, Chromatin Immunoprecipitation, Electrophoretic Mobility Shift Assay, Real-time Polymerase Chain Reaction, Isolation, Amplification, Transfection, Incubation, Labeling

    PCR products from amplification with OMPf (upper panel) and BMSf (lower panel) primers of genomic extracts of CO-oxidizing cultures. Products were visualized with ethidium bromide after gel electrophoresis using 1% agarose. Lanes A and L contain a 100-bp ladder (Promega, Inc.); 500- and 1,500-bp markers are indicated by arrows on the right side of the upper panel. Lane B, Oligotropha carboxidotropha ; lane C, M. smegmatis ; lane D, M. avium ; lane E, B. fungorum LB400; lane F, Stappia stellulata ; lane G, Mesorhizobium sp. strain NMB1; lane H, Bradyrhizobium sp. strain CPP; lane I, Aminobacter sp. strain COX; lane J, L. anuloederans ; lane K, negative control.

    Journal: Applied and Environmental Microbiology

    Article Title: Molecular and Culture-Based Analyses of Aerobic Carbon Monoxide Oxidizer Diversity+

    doi: 10.1128/AEM.69.12.7257-7265.2003

    Figure Lengend Snippet: PCR products from amplification with OMPf (upper panel) and BMSf (lower panel) primers of genomic extracts of CO-oxidizing cultures. Products were visualized with ethidium bromide after gel electrophoresis using 1% agarose. Lanes A and L contain a 100-bp ladder (Promega, Inc.); 500- and 1,500-bp markers are indicated by arrows on the right side of the upper panel. Lane B, Oligotropha carboxidotropha ; lane C, M. smegmatis ; lane D, M. avium ; lane E, B. fungorum LB400; lane F, Stappia stellulata ; lane G, Mesorhizobium sp. strain NMB1; lane H, Bradyrhizobium sp. strain CPP; lane I, Aminobacter sp. strain COX; lane J, L. anuloederans ; lane K, negative control.

    Article Snippet: PCR products were visualized with ethidium bromide gel electrophoresis and then purified with MoBio PCR cleanup kits.

    Techniques: Polymerase Chain Reaction, Amplification, Nucleic Acid Electrophoresis, Conditioned Place Preference, Negative Control